Determination
Different sensitive and selective spectrophotometric methods for the determination of ofloxacin and dexamethasone in their binary mixture were presented. Ofloxacin was determined simply by zero order at its λmax 293.4 nm in a linear range of 1.5–12 μg mL−1 with mean percentage recovery of 100.07 ± 0...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2013-12-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093113000240 |
| _version_ | 1797971549399023616 |
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| author | M. Nebsen Ghada M. Elsayed M. AbdelKawy S.Z. ELkhateeb |
| author_facet | M. Nebsen Ghada M. Elsayed M. AbdelKawy S.Z. ELkhateeb |
| author_sort | M. Nebsen |
| collection | DOAJ |
| description | Different sensitive and selective spectrophotometric methods for the determination of ofloxacin and dexamethasone in their binary mixture were presented. Ofloxacin was determined simply by zero order at its λmax 293.4 nm in a linear range of 1.5–12 μg mL−1 with mean percentage recovery of 100.07 ± 0.66% without any interference of dexamethasone even in low or high concentrations. Dexamethasone was determined by first derivative of ratio spectra 1DD at 266.5 nm, ratio subtraction and mean centering at 243 nm with methods in a linear range of 2.5–27.5 μg mL−1 with mean percentage recoveries of 100.09 ± 0.70%, 100.00 ± 0.72% and 99.92 ± 0.62, respectively. These methods were applied to the analysis of pharmaceutical dosage form and bulk powder where good recoveries were obtained. The proposed methods were validated according to USP guidelines. |
| first_indexed | 2024-04-11T03:34:24Z |
| format | Article |
| id | doaj.art-4c7bd7c5dfb14c2bbcb8e3e77fb222b9 |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T03:34:24Z |
| publishDate | 2013-12-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-4c7bd7c5dfb14c2bbcb8e3e77fb222b92023-01-02T05:41:00ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312013-12-0151217518410.1016/j.bfopcu.2013.04.004DeterminationM. Nebsen0Ghada M. Elsayed1M. AbdelKawy2S.Z. ELkhateeb3Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, 11562 Cairo, EgyptAnalytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, 11562 Cairo, EgyptAnalytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, 11562 Cairo, EgyptAnalytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, 11562 Cairo, EgyptDifferent sensitive and selective spectrophotometric methods for the determination of ofloxacin and dexamethasone in their binary mixture were presented. Ofloxacin was determined simply by zero order at its λmax 293.4 nm in a linear range of 1.5–12 μg mL−1 with mean percentage recovery of 100.07 ± 0.66% without any interference of dexamethasone even in low or high concentrations. Dexamethasone was determined by first derivative of ratio spectra 1DD at 266.5 nm, ratio subtraction and mean centering at 243 nm with methods in a linear range of 2.5–27.5 μg mL−1 with mean percentage recoveries of 100.09 ± 0.70%, 100.00 ± 0.72% and 99.92 ± 0.62, respectively. These methods were applied to the analysis of pharmaceutical dosage form and bulk powder where good recoveries were obtained. The proposed methods were validated according to USP guidelines.http://www.sciencedirect.com/science/article/pii/S1110093113000240OfloxacinDexamethasoneDerivative ratioRatio subtractionMean centering |
| spellingShingle | M. Nebsen Ghada M. Elsayed M. AbdelKawy S.Z. ELkhateeb Determination Bulletin of Faculty of Pharmacy Cairo University Ofloxacin Dexamethasone Derivative ratio Ratio subtraction Mean centering |
| title | Determination |
| title_full | Determination |
| title_fullStr | Determination |
| title_full_unstemmed | Determination |
| title_short | Determination |
| title_sort | determination |
| topic | Ofloxacin Dexamethasone Derivative ratio Ratio subtraction Mean centering |
| url | http://www.sciencedirect.com/science/article/pii/S1110093113000240 |
| work_keys_str_mv | AT mnebsen determination AT ghadamelsayed determination AT mabdelkawy determination AT szelkhateeb determination |