Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system
A TNFRSF11B (TNF Receptor Superfamily Member 11b) gene encodes a soluble decoy receptor, osteoprotegerin (OPG), which has a key role in repressing osteoclast differentiation. In this report, we generated a biallelic knock-out hiPSC line for the TNFRSF11B gene via CRISPR/Cas9. When TNFRSF11B Knock-ou...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123001538 |
| _version_ | 1797692045682278400 |
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| author | Jujin Jeong Dahye Lee Byeong-Cheol Park Tae-Hoon Lee Sang-Wook Park |
| author_facet | Jujin Jeong Dahye Lee Byeong-Cheol Park Tae-Hoon Lee Sang-Wook Park |
| author_sort | Jujin Jeong |
| collection | DOAJ |
| description | A TNFRSF11B (TNF Receptor Superfamily Member 11b) gene encodes a soluble decoy receptor, osteoprotegerin (OPG), which has a key role in repressing osteoclast differentiation. In this report, we generated a biallelic knock-out hiPSC line for the TNFRSF11B gene via CRISPR/Cas9. When TNFRSF11B Knock-out hiPSCs were differentiated into mesenchymal progenitor cells (MPCs), the expression level of OPG was significantly decreased compared to normal hiPSC-derived MPCs. This knock-out hiPSCs will provide a chance to study Paget disease of bone 5 (juvenile Paget disease). |
| first_indexed | 2024-03-12T02:22:00Z |
| format | Article |
| id | doaj.art-4c7c758b4a2340d08c12f030683f14bc |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-12T02:22:00Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-4c7c758b4a2340d08c12f030683f14bc2023-09-06T04:50:51ZengElsevierStem Cell Research1873-50612023-09-0171103167Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 systemJujin Jeong0Dahye Lee1Byeong-Cheol Park2Tae-Hoon Lee3Sang-Wook Park4Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South KoreaDepartment of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South KoreaDepartment of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South KoreaCorresponding authors.; Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South KoreaCorresponding authors.; Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South KoreaA TNFRSF11B (TNF Receptor Superfamily Member 11b) gene encodes a soluble decoy receptor, osteoprotegerin (OPG), which has a key role in repressing osteoclast differentiation. In this report, we generated a biallelic knock-out hiPSC line for the TNFRSF11B gene via CRISPR/Cas9. When TNFRSF11B Knock-out hiPSCs were differentiated into mesenchymal progenitor cells (MPCs), the expression level of OPG was significantly decreased compared to normal hiPSC-derived MPCs. This knock-out hiPSCs will provide a chance to study Paget disease of bone 5 (juvenile Paget disease).http://www.sciencedirect.com/science/article/pii/S1873506123001538 |
| spellingShingle | Jujin Jeong Dahye Lee Byeong-Cheol Park Tae-Hoon Lee Sang-Wook Park Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system Stem Cell Research |
| title | Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system |
| title_full | Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system |
| title_fullStr | Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system |
| title_full_unstemmed | Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system |
| title_short | Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system |
| title_sort | establishment of a tnfrsf11b knock out human induced pluripotent stem cell line kscbi002 b 2 via crispr cas9 system |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123001538 |
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