Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic Acid
The present study investigated the transcriptomic and metabolomic changes elicited in tomato plants (Solanum lycopersicum cv. Micro-Tom) following treatments with the biocontrol agent Trichoderma harzianum strain M10 or its purified secondary metabolite harzianic acid (HA), in the presence or the ab...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01966/full |
| _version_ | 1811276878509506560 |
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| author | Gelsomina Manganiello Adriana Sacco Maria R. Ercolano Francesco Vinale Stefania Lanzuise Alberto Pascale Mauro Napolitano Nadia Lombardi Matteo Lorito Matteo Lorito Matteo Lorito Sheridan L. Woo Sheridan L. Woo Sheridan L. Woo |
| author_facet | Gelsomina Manganiello Adriana Sacco Maria R. Ercolano Francesco Vinale Stefania Lanzuise Alberto Pascale Mauro Napolitano Nadia Lombardi Matteo Lorito Matteo Lorito Matteo Lorito Sheridan L. Woo Sheridan L. Woo Sheridan L. Woo |
| author_sort | Gelsomina Manganiello |
| collection | DOAJ |
| description | The present study investigated the transcriptomic and metabolomic changes elicited in tomato plants (Solanum lycopersicum cv. Micro-Tom) following treatments with the biocontrol agent Trichoderma harzianum strain M10 or its purified secondary metabolite harzianic acid (HA), in the presence or the absence of the soil-borne pathogen Rhizoctonia solani. Transcriptomic analysis allowed the identification of differentially expressed genes (DEGs) that play a pivotal role in resistance to biotic stress. Overall, the results support the ability of T. harzianum M10 to activate defense responses in infected tomato plants. An induction of hormone-mediated signaling was observed, as shown by the up-regulation of genes involved in the ethylene and jasmonate (ET/JA) and salicylic acid (SA)-mediated signaling pathways. Further, the protective action of T. harzianum on the host was revealed by the over-expression of genes able to detoxify cells from reactive oxygen species (ROS). On the other hand, HA treatment also stimulated tomato response to the pathogen by inducing the expression of several genes involved in defense response (including protease inhibitors, resistance proteins like CC-NBS-LRR) and hormone interplay. The accumulation of steroidal glycoalkaloids in the plant after treatments with either T. harzianum or HA, as determined by metabolomic analysis, confirmed the complexity of the plant response to beneficial microbes, demonstrating that these microorganisms are also capable of activating the chemical defenses. |
| first_indexed | 2024-04-13T00:05:44Z |
| format | Article |
| id | doaj.art-4c7ff002df6f40098dd1bdbb5fbee157 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T00:05:44Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4c7ff002df6f40098dd1bdbb5fbee1572022-12-22T03:11:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-08-01910.3389/fmicb.2018.01966390198Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic AcidGelsomina Manganiello0Adriana Sacco1Maria R. Ercolano2Francesco Vinale3Stefania Lanzuise4Alberto Pascale5Mauro Napolitano6Nadia Lombardi7Matteo Lorito8Matteo Lorito9Matteo Lorito10Sheridan L. Woo11Sheridan L. Woo12Sheridan L. Woo13Department of Agricultural Sciences, University of Naples Federico II, Portici, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, ItalyNational Research Council, Institute for Sustainable Plant Protection, Portici, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, ItalyNational Research Council, Institute for Sustainable Plant Protection, Portici, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, ItalyNational Research Council, Institute for Sustainable Plant Protection, Portici, ItalyTask Force on Microbiome Studies, University of Naples Federico II, Naples, ItalyNational Research Council, Institute for Sustainable Plant Protection, Portici, ItalyTask Force on Microbiome Studies, University of Naples Federico II, Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Naples, ItalyThe present study investigated the transcriptomic and metabolomic changes elicited in tomato plants (Solanum lycopersicum cv. Micro-Tom) following treatments with the biocontrol agent Trichoderma harzianum strain M10 or its purified secondary metabolite harzianic acid (HA), in the presence or the absence of the soil-borne pathogen Rhizoctonia solani. Transcriptomic analysis allowed the identification of differentially expressed genes (DEGs) that play a pivotal role in resistance to biotic stress. Overall, the results support the ability of T. harzianum M10 to activate defense responses in infected tomato plants. An induction of hormone-mediated signaling was observed, as shown by the up-regulation of genes involved in the ethylene and jasmonate (ET/JA) and salicylic acid (SA)-mediated signaling pathways. Further, the protective action of T. harzianum on the host was revealed by the over-expression of genes able to detoxify cells from reactive oxygen species (ROS). On the other hand, HA treatment also stimulated tomato response to the pathogen by inducing the expression of several genes involved in defense response (including protease inhibitors, resistance proteins like CC-NBS-LRR) and hormone interplay. The accumulation of steroidal glycoalkaloids in the plant after treatments with either T. harzianum or HA, as determined by metabolomic analysis, confirmed the complexity of the plant response to beneficial microbes, demonstrating that these microorganisms are also capable of activating the chemical defenses.https://www.frontiersin.org/article/10.3389/fmicb.2018.01966/fullTrichoderma harzianumsecondary metabolitestomatobiological controlbeneficial microbesresistance response |
| spellingShingle | Gelsomina Manganiello Adriana Sacco Maria R. Ercolano Francesco Vinale Stefania Lanzuise Alberto Pascale Mauro Napolitano Nadia Lombardi Matteo Lorito Matteo Lorito Matteo Lorito Sheridan L. Woo Sheridan L. Woo Sheridan L. Woo Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic Acid Frontiers in Microbiology Trichoderma harzianum secondary metabolites tomato biological control beneficial microbes resistance response |
| title | Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic Acid |
| title_full | Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic Acid |
| title_fullStr | Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic Acid |
| title_full_unstemmed | Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic Acid |
| title_short | Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic Acid |
| title_sort | modulation of tomato response to rhizoctonia solani by trichoderma harzianum and its secondary metabolite harzianic acid |
| topic | Trichoderma harzianum secondary metabolites tomato biological control beneficial microbes resistance response |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01966/full |
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