Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid Lines
The selection with co-dominant SSR markers existent on the market, accomplished in dihaploid wheat lines, offers the opportunity to rapidly stabilize the selected resistant individuals into homozygous estate. As biological material there were used 21 resistant and two sensitive dihaploid lines. All...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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AcademicPres
2009-06-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| Online Access: | http://www.notulaebotanicae.ro/index.php/nbha/article/view/3131 |
| _version_ | 1818417773193199616 |
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| author | Constantin BOTEZ Doru PAMFIL Dana CURTICIU Laura Cristina COTA Nicolae N. SAULESCU |
| author_facet | Constantin BOTEZ Doru PAMFIL Dana CURTICIU Laura Cristina COTA Nicolae N. SAULESCU |
| author_sort | Constantin BOTEZ |
| collection | DOAJ |
| description | The selection with co-dominant SSR markers existent on the market, accomplished in dihaploid wheat lines, offers the opportunity to rapidly stabilize the selected resistant individuals into homozygous estate. As biological material there were used 21 resistant and two sensitive dihaploid lines. All dihaploid lines tested, with field resistance to Septoria tritici leaf blotch, expressed at least once such a resistant gene. The most frequently there were encountered in the tested material the Stb 7 and Stb 8 leaf blotch resistant genes. The size of amplification products specific for SSR markers linked with Stb 7 and Stb 8 resistant genes were similar to those obtained by Adhikari et al. (2004) but the size of amplification products linked with Stb 2 resistant genes were quite different, probably due to the polymorphism that characterizes microsatellite markers at the allelic level. The Stb3, Stb 4 and Stb 6 resistant genes were totally absent. The results obtained for Stb 1 and Stb 5 were inconclusive. |
| first_indexed | 2024-12-14T12:12:06Z |
| format | Article |
| id | doaj.art-4c88fadbd41a4431b741e4a82265eb6e |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-12-14T12:12:06Z |
| publishDate | 2009-06-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-4c88fadbd41a4431b741e4a82265eb6e2022-12-21T23:01:42ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092009-06-0137125325510.15835/nbha37131313356Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid LinesConstantin BOTEZ0Doru PAMFIL1Dana CURTICIU2Laura Cristina COTA3Nicolae N. SAULESCU4University of Agricultural Science and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Manastur St., 400372University of Agricultural Science and Veterinary Medicine, 3-5 Manastur St. Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Manastur St., 400372Res. Inst. for Cereals and Industrial Crops, Fundulea, 8264 Calarasi CountyThe selection with co-dominant SSR markers existent on the market, accomplished in dihaploid wheat lines, offers the opportunity to rapidly stabilize the selected resistant individuals into homozygous estate. As biological material there were used 21 resistant and two sensitive dihaploid lines. All dihaploid lines tested, with field resistance to Septoria tritici leaf blotch, expressed at least once such a resistant gene. The most frequently there were encountered in the tested material the Stb 7 and Stb 8 leaf blotch resistant genes. The size of amplification products specific for SSR markers linked with Stb 7 and Stb 8 resistant genes were similar to those obtained by Adhikari et al. (2004) but the size of amplification products linked with Stb 2 resistant genes were quite different, probably due to the polymorphism that characterizes microsatellite markers at the allelic level. The Stb3, Stb 4 and Stb 6 resistant genes were totally absent. The results obtained for Stb 1 and Stb 5 were inconclusive.http://www.notulaebotanicae.ro/index.php/nbha/article/view/3131 |
| spellingShingle | Constantin BOTEZ Doru PAMFIL Dana CURTICIU Laura Cristina COTA Nicolae N. SAULESCU Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid Lines Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| title | Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid Lines |
| title_full | Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid Lines |
| title_fullStr | Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid Lines |
| title_full_unstemmed | Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid Lines |
| title_short | Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid Lines |
| title_sort | marker assisted selection for septoria tritici resistance in wheat dihaploid lines |
| url | http://www.notulaebotanicae.ro/index.php/nbha/article/view/3131 |
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