Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies
Influenza virus HA-specific antibodies can be detected via the hemagglutination inhibition (HI) assay, the neutralization (NT) assay, and the enzyme-linked immunosorbent assay (ELISA). However, these assays have some drawbacks, including narrow dynamic range and the requirement for large amounts of...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2021-06-01
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| Series: | mSphere |
| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.01342-20 |
| _version_ | 1819334263434117120 |
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| author | Tomoko Honda Sumiko Gomi Daisuke Yamane Fumihiko Yasui Takuya Yamamoto Tsubasa Munakata Yasushi Itoh Kazumasa Ogasawara Takahiro Sanada Kenzaburo Yamaji Yasuhiro Yasutomi Kyoko Tsukiyama-Kohara Michinori Kohara |
| author_facet | Tomoko Honda Sumiko Gomi Daisuke Yamane Fumihiko Yasui Takuya Yamamoto Tsubasa Munakata Yasushi Itoh Kazumasa Ogasawara Takahiro Sanada Kenzaburo Yamaji Yasuhiro Yasutomi Kyoko Tsukiyama-Kohara Michinori Kohara |
| author_sort | Tomoko Honda |
| collection | DOAJ |
| description | Influenza virus HA-specific antibodies can be detected via the hemagglutination inhibition (HI) assay, the neutralization (NT) assay, and the enzyme-linked immunosorbent assay (ELISA). However, these assays have some drawbacks, including narrow dynamic range and the requirement for large amounts of sera. |
| first_indexed | 2024-12-24T14:59:19Z |
| format | Article |
| id | doaj.art-4c8983ceadeb457a9300b83eeaa4233b |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-24T14:59:19Z |
| publishDate | 2021-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-4c8983ceadeb457a9300b83eeaa4233b2022-12-21T16:50:44ZengAmerican Society for MicrobiologymSphere2379-50422021-06-016310.1128/mSphere.01342-20Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA AntibodiesTomoko Honda0Sumiko Gomi1Daisuke Yamane2Fumihiko Yasui3Takuya Yamamoto4Tsubasa Munakata5Yasushi Itoh6https://orcid.org/0000-0001-7462-2193Kazumasa Ogasawara7Takahiro Sanada8Kenzaburo Yamaji9Yasuhiro Yasutomi10Kyoko Tsukiyama-Kohara11Michinori Kohara12https://orcid.org/0000-0002-2130-5837Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanDepartment of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanDepartment of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanDepartment of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanLaboratory of Immunosenescence, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, JapanDepartment of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanDivision of Pathogenesis and Disease Regulation, Department of Pathology, Shiga University of Medical Science, Setatsukinowa, Otsu, Shiga, JapanDivision of Pathogenesis and Disease Regulation, Department of Pathology, Shiga University of Medical Science, Setatsukinowa, Otsu, Shiga, JapanDepartment of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanDepartment of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanTsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, JapanTransboundary Animal Diseases Centre, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Kagoshima, JapanDepartment of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanInfluenza virus HA-specific antibodies can be detected via the hemagglutination inhibition (HI) assay, the neutralization (NT) assay, and the enzyme-linked immunosorbent assay (ELISA). However, these assays have some drawbacks, including narrow dynamic range and the requirement for large amounts of sera.https://journals.asm.org/doi/10.1128/mSphere.01342-20 |
| spellingShingle | Tomoko Honda Sumiko Gomi Daisuke Yamane Fumihiko Yasui Takuya Yamamoto Tsubasa Munakata Yasushi Itoh Kazumasa Ogasawara Takahiro Sanada Kenzaburo Yamaji Yasuhiro Yasutomi Kyoko Tsukiyama-Kohara Michinori Kohara Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies mSphere |
| title | Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies |
| title_full | Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies |
| title_fullStr | Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies |
| title_full_unstemmed | Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies |
| title_short | Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies |
| title_sort | development and characterization of a highly sensitive nanoluciferase based immunoprecipitation system for the detection of anti influenza virus ha antibodies |
| url | https://journals.asm.org/doi/10.1128/mSphere.01342-20 |
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