Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid Arthritis
Long non-coding RNAs (lncRNAs) are increasingly recognized to play important roles in multiple autoimmune diseases. This study aimed to evaluate the association of four lncRNAs (ANRIL, lnc-DC, MALAT1, ZFAS1) genes single nucleotide polymorphisms (SNPs) with susceptibility to rheumatoid arthritis (RA...
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| Format: | Article |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.02529/full |
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| author | Tian-Ping Zhang Tian-Ping Zhang Bang-Qiang Zhu Sha-Sha Tao Yin-Guang Fan Xiao-Mei Li Hai-Feng Pan Dong-Qing Ye |
| author_facet | Tian-Ping Zhang Tian-Ping Zhang Bang-Qiang Zhu Sha-Sha Tao Yin-Guang Fan Xiao-Mei Li Hai-Feng Pan Dong-Qing Ye |
| author_sort | Tian-Ping Zhang |
| collection | DOAJ |
| description | Long non-coding RNAs (lncRNAs) are increasingly recognized to play important roles in multiple autoimmune diseases. This study aimed to evaluate the association of four lncRNAs (ANRIL, lnc-DC, MALAT1, ZFAS1) genes single nucleotide polymorphisms (SNPs) with susceptibility to rheumatoid arthritis (RA) patients, as well as their expression levels. Seventeen SNPs of the four lncRNAs were genotyped in a cohort of 660 RA patients and 710 controls using improved multiple ligase detection reaction (iMLDR). The lncRNAs expressions in peripheral blood mononuclear cells (PBMCs) from 120 RA patients and 120 controls were detected by qRT-PCR. No significant differences were found for the allele and genotype frequencies distribution of ANRIL SNPs (rs1412830, rs944796, rs61271866, rs2518723, rs3217992), lnc-DC SNPs (rs7217280, rs10515177), MALAT1 SNPs (rs619586, rs4102217, rs591291, rs11227209, rs35138901), ZFAS1 SNPs (rs237742, rs73116127, rs6125607, rs6125608) between RA patients and normal controls (all P > 0.05). The genotype effects of dominant and recessive models were also evaluated, but no significant association was found. In addition, our results demonstrated that the rs944796 G allele, rs2518723 T allele, rs3217992 T allele frequencies were significantly associated with anti-CCP in RA patients (all P < 0.05). The haplotype CGTA frequency for ZFAS1 was significantly higher in RA patients (P = 0.036). Compared with normal controls, the expression levels of ANRIL, lnc-DC, MALAT1, ZFAS1 in PBMCs were significantly reduced in RA patients (all P < 0.001). Moreover, ZFAS1 expression was negatively associated with CRP in RA patients (P = 0.002). In summary, ANRIL, lnc-DC, MALAT1, and ZFAS1 genes SNPs were not associated with RA susceptibility, while altered ANRIL, lnc-DC, MALAT1, ZFAS1 levels in RA patients suggested that these lncRNAs might play a role in RA. |
| first_indexed | 2024-04-12T21:56:07Z |
| format | Article |
| id | doaj.art-4c89f6db2d414f5892eae7a940f611d6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T21:56:07Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-4c89f6db2d414f5892eae7a940f611d62022-12-22T03:15:19ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-10-011010.3389/fimmu.2019.02529447661Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid ArthritisTian-Ping Zhang0Tian-Ping Zhang1Bang-Qiang Zhu2Sha-Sha Tao3Yin-Guang Fan4Xiao-Mei Li5Hai-Feng Pan6Dong-Qing Ye7Anhui Province Key Laboratory of Major Autoimmune Diseases, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, ChinaDepartment of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaAnhui Province Key Laboratory of Major Autoimmune Diseases, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, ChinaAnhui Province Key Laboratory of Major Autoimmune Diseases, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, ChinaAnhui Province Key Laboratory of Major Autoimmune Diseases, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, ChinaDepartment of Rheumatology, The First Affiliated Hospital of University of Science and Technology of China, Hefei, ChinaAnhui Province Key Laboratory of Major Autoimmune Diseases, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, ChinaAnhui Province Key Laboratory of Major Autoimmune Diseases, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, ChinaLong non-coding RNAs (lncRNAs) are increasingly recognized to play important roles in multiple autoimmune diseases. This study aimed to evaluate the association of four lncRNAs (ANRIL, lnc-DC, MALAT1, ZFAS1) genes single nucleotide polymorphisms (SNPs) with susceptibility to rheumatoid arthritis (RA) patients, as well as their expression levels. Seventeen SNPs of the four lncRNAs were genotyped in a cohort of 660 RA patients and 710 controls using improved multiple ligase detection reaction (iMLDR). The lncRNAs expressions in peripheral blood mononuclear cells (PBMCs) from 120 RA patients and 120 controls were detected by qRT-PCR. No significant differences were found for the allele and genotype frequencies distribution of ANRIL SNPs (rs1412830, rs944796, rs61271866, rs2518723, rs3217992), lnc-DC SNPs (rs7217280, rs10515177), MALAT1 SNPs (rs619586, rs4102217, rs591291, rs11227209, rs35138901), ZFAS1 SNPs (rs237742, rs73116127, rs6125607, rs6125608) between RA patients and normal controls (all P > 0.05). The genotype effects of dominant and recessive models were also evaluated, but no significant association was found. In addition, our results demonstrated that the rs944796 G allele, rs2518723 T allele, rs3217992 T allele frequencies were significantly associated with anti-CCP in RA patients (all P < 0.05). The haplotype CGTA frequency for ZFAS1 was significantly higher in RA patients (P = 0.036). Compared with normal controls, the expression levels of ANRIL, lnc-DC, MALAT1, ZFAS1 in PBMCs were significantly reduced in RA patients (all P < 0.001). Moreover, ZFAS1 expression was negatively associated with CRP in RA patients (P = 0.002). In summary, ANRIL, lnc-DC, MALAT1, and ZFAS1 genes SNPs were not associated with RA susceptibility, while altered ANRIL, lnc-DC, MALAT1, ZFAS1 levels in RA patients suggested that these lncRNAs might play a role in RA.https://www.frontiersin.org/article/10.3389/fimmu.2019.02529/fullANRILlnc-DCMALAT1ZFAS1single nucleotide polymorphismsrheumatoid arthritis |
| spellingShingle | Tian-Ping Zhang Tian-Ping Zhang Bang-Qiang Zhu Sha-Sha Tao Yin-Guang Fan Xiao-Mei Li Hai-Feng Pan Dong-Qing Ye Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid Arthritis Frontiers in Immunology ANRIL lnc-DC MALAT1 ZFAS1 single nucleotide polymorphisms rheumatoid arthritis |
| title | Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid Arthritis |
| title_full | Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid Arthritis |
| title_fullStr | Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid Arthritis |
| title_full_unstemmed | Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid Arthritis |
| title_short | Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid Arthritis |
| title_sort | long non coding rnas genes polymorphisms and their expression levels in patients with rheumatoid arthritis |
| topic | ANRIL lnc-DC MALAT1 ZFAS1 single nucleotide polymorphisms rheumatoid arthritis |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.02529/full |
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