Characterization and Mapping of a Rolling Leaf Mutant Allele <i>rlT73</i> on Chromosome 1BL of Wheat
Leaf rolling is regarded as an important morphological trait in wheat breeding. Moderate leaf rolling is helpful to keep leaves upright and improve the photosynthesis of plants, leading to increased yield. However, studies on the identification of genomic regions/genes associated with rolling leaf h...
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MDPI AG
2024-04-01
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| Series: | International Journal of Molecular Sciences |
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| author | Lin Huang Meijuan Gan Wenzhuo Zhao Yanling Hu Lilin Du Yuqin Li Kanghui Zeng Dandan Wu Ming Hao Shunzong Ning Zhongwei Yuan Lihua Feng Lianquan Zhang Bihua Wu Dengcai Liu |
| author_facet | Lin Huang Meijuan Gan Wenzhuo Zhao Yanling Hu Lilin Du Yuqin Li Kanghui Zeng Dandan Wu Ming Hao Shunzong Ning Zhongwei Yuan Lihua Feng Lianquan Zhang Bihua Wu Dengcai Liu |
| author_sort | Lin Huang |
| collection | DOAJ |
| description | Leaf rolling is regarded as an important morphological trait in wheat breeding. Moderate leaf rolling is helpful to keep leaves upright and improve the photosynthesis of plants, leading to increased yield. However, studies on the identification of genomic regions/genes associated with rolling leaf have been reported less frequently in wheat. In this study, a rolling leaf mutant, T73, which has paired spikelets, dwarfism, and delayed heading traits, was obtained from a common wheat landrace through ethyl methanesulfonate mutagenesis. The <i>rlT73</i> mutation caused an increase in the number of epidermal cells on the abaxial side and the shrinkage of bulliform cells on the adaxial side, leading to an adaxially rolling leaf phenotype. Genetic analysis showed that the rolling leaf phenotype was controlled by a single recessive gene. Further Wheat55K single nucleotide polymorphism array-based bulked segregant analysis and molecular marker mapping delimited <i>rlT73</i> to a physical interval of 300.29–318.33 Mb on the chromosome arm 1BL in the Chinese Spring genome. We show that a point mutation at the miRNA165/166 binding site of the HD zipper class III transcription factor on 1BL altered its transcriptional level, which may be responsible for the rolling leaf phenotype. Our results suggest the important role of <i>rlT73</i> in regulating wheat leaf development and the potential of miRNA-based gene regulation for crop trait improvement. |
| first_indexed | 2024-04-24T10:42:11Z |
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| language | English |
| last_indexed | 2024-04-24T10:42:11Z |
| publishDate | 2024-04-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4c8d830a81c44f168a1f36742a2183852024-04-12T13:20:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-04-01257410310.3390/ijms25074103Characterization and Mapping of a Rolling Leaf Mutant Allele <i>rlT73</i> on Chromosome 1BL of WheatLin Huang0Meijuan Gan1Wenzhuo Zhao2Yanling Hu3Lilin Du4Yuqin Li5Kanghui Zeng6Dandan Wu7Ming Hao8Shunzong Ning9Zhongwei Yuan10Lihua Feng11Lianquan Zhang12Bihua Wu13Dengcai Liu14State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaLeaf rolling is regarded as an important morphological trait in wheat breeding. Moderate leaf rolling is helpful to keep leaves upright and improve the photosynthesis of plants, leading to increased yield. However, studies on the identification of genomic regions/genes associated with rolling leaf have been reported less frequently in wheat. In this study, a rolling leaf mutant, T73, which has paired spikelets, dwarfism, and delayed heading traits, was obtained from a common wheat landrace through ethyl methanesulfonate mutagenesis. The <i>rlT73</i> mutation caused an increase in the number of epidermal cells on the abaxial side and the shrinkage of bulliform cells on the adaxial side, leading to an adaxially rolling leaf phenotype. Genetic analysis showed that the rolling leaf phenotype was controlled by a single recessive gene. Further Wheat55K single nucleotide polymorphism array-based bulked segregant analysis and molecular marker mapping delimited <i>rlT73</i> to a physical interval of 300.29–318.33 Mb on the chromosome arm 1BL in the Chinese Spring genome. We show that a point mutation at the miRNA165/166 binding site of the HD zipper class III transcription factor on 1BL altered its transcriptional level, which may be responsible for the rolling leaf phenotype. Our results suggest the important role of <i>rlT73</i> in regulating wheat leaf development and the potential of miRNA-based gene regulation for crop trait improvement.https://www.mdpi.com/1422-0067/25/7/4103wheatrolling leafmiRNAHD zipper class III transcription factor |
| spellingShingle | Lin Huang Meijuan Gan Wenzhuo Zhao Yanling Hu Lilin Du Yuqin Li Kanghui Zeng Dandan Wu Ming Hao Shunzong Ning Zhongwei Yuan Lihua Feng Lianquan Zhang Bihua Wu Dengcai Liu Characterization and Mapping of a Rolling Leaf Mutant Allele <i>rlT73</i> on Chromosome 1BL of Wheat International Journal of Molecular Sciences wheat rolling leaf miRNA HD zipper class III transcription factor |
| title | Characterization and Mapping of a Rolling Leaf Mutant Allele <i>rlT73</i> on Chromosome 1BL of Wheat |
| title_full | Characterization and Mapping of a Rolling Leaf Mutant Allele <i>rlT73</i> on Chromosome 1BL of Wheat |
| title_fullStr | Characterization and Mapping of a Rolling Leaf Mutant Allele <i>rlT73</i> on Chromosome 1BL of Wheat |
| title_full_unstemmed | Characterization and Mapping of a Rolling Leaf Mutant Allele <i>rlT73</i> on Chromosome 1BL of Wheat |
| title_short | Characterization and Mapping of a Rolling Leaf Mutant Allele <i>rlT73</i> on Chromosome 1BL of Wheat |
| title_sort | characterization and mapping of a rolling leaf mutant allele i rlt73 i on chromosome 1bl of wheat |
| topic | wheat rolling leaf miRNA HD zipper class III transcription factor |
| url | https://www.mdpi.com/1422-0067/25/7/4103 |
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