Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study.
In this paper, we present a combined theoretical and experimental study of the propagation of calcium signals in multicellular structures composed of human endothelial cells. We consider multicellular structures composed of a single chain of cells as well as a chain of cells with a side branch, name...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Computational Biology |
| Online Access: | https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1002847&type=printable |
| _version_ | 1826799397004902400 |
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| author | Juexuan Long Michael Junkin Pak Kin Wong James Hoying Pierre Deymier |
| author_facet | Juexuan Long Michael Junkin Pak Kin Wong James Hoying Pierre Deymier |
| author_sort | Juexuan Long |
| collection | DOAJ |
| description | In this paper, we present a combined theoretical and experimental study of the propagation of calcium signals in multicellular structures composed of human endothelial cells. We consider multicellular structures composed of a single chain of cells as well as a chain of cells with a side branch, namely a "T" structure. In the experiments, we investigate the result of applying mechano-stimulation to induce signaling in the form of calcium waves along the chain and the effect of single and dual stimulation of the multicellular structure. The experimental results provide evidence of an effect of architecture on the propagation of calcium waves. Simulations based on a model of calcium-induced calcium release and cell-to-cell diffusion through gap junctions shows that the propagation of calcium waves is dependent upon the competition between intracellular calcium regulation and architecture-dependent intercellular diffusion. |
| first_indexed | 2024-12-12T15:30:45Z |
| format | Article |
| id | doaj.art-4c9372e9a88d4abd953c6f6d93fc189f |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2025-03-17T00:34:26Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-4c9372e9a88d4abd953c6f6d93fc189f2025-02-21T05:32:01ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582012-01-01812e100284710.1371/journal.pcbi.1002847Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study.Juexuan LongMichael JunkinPak Kin WongJames HoyingPierre DeymierIn this paper, we present a combined theoretical and experimental study of the propagation of calcium signals in multicellular structures composed of human endothelial cells. We consider multicellular structures composed of a single chain of cells as well as a chain of cells with a side branch, namely a "T" structure. In the experiments, we investigate the result of applying mechano-stimulation to induce signaling in the form of calcium waves along the chain and the effect of single and dual stimulation of the multicellular structure. The experimental results provide evidence of an effect of architecture on the propagation of calcium waves. Simulations based on a model of calcium-induced calcium release and cell-to-cell diffusion through gap junctions shows that the propagation of calcium waves is dependent upon the competition between intracellular calcium regulation and architecture-dependent intercellular diffusion.https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1002847&type=printable |
| spellingShingle | Juexuan Long Michael Junkin Pak Kin Wong James Hoying Pierre Deymier Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study. PLoS Computational Biology |
| title | Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study. |
| title_full | Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study. |
| title_fullStr | Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study. |
| title_full_unstemmed | Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study. |
| title_short | Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study. |
| title_sort | calcium wave propagation in networks of endothelial cells model based theoretical and experimental study |
| url | https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1002847&type=printable |
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