Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to <i>Stenotrophomonas maltophilia</i> Clinical Ocular Isolates
Recent acknowledgment that multidrug resistant <i>Stenotrophomonas maltophilia</i> strains can cause severe infections has led to increasing global interest in addressing its pathogenicity. While being primarily associated with hospital-acquired respiratory tract infections, this bacteri...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/4/709 |
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| author | Dragica Damnjanović Xabier Vázquez-Campos Lisa Elliott Mark Willcox Wallace J. Bridge |
| author_facet | Dragica Damnjanović Xabier Vázquez-Campos Lisa Elliott Mark Willcox Wallace J. Bridge |
| author_sort | Dragica Damnjanović |
| collection | DOAJ |
| description | Recent acknowledgment that multidrug resistant <i>Stenotrophomonas maltophilia</i> strains can cause severe infections has led to increasing global interest in addressing its pathogenicity. While being primarily associated with hospital-acquired respiratory tract infections, this bacterial species is also relevant to ophthalmology, particularly to contact lens-related diseases. In the current study, the capacity of <i>Stenotrophomonas</i> phage vB_SmaM_Ps15 to infect ocular <i>S. maltophilia</i> strains was investigated to explore its future potential as a phage therapeutic. The phage proved to be lytic to a range of clinical isolates collected in Australia from eye swabs, contact lenses and contact lens cases that had previously shown to be resistant to several antibiotics and multipurpose contact lenses disinfectant solutions. Morphological analysis by transmission electron microscopy placed the phage into the <i>Myoviridae</i> family. Its genome size was 161,350 bp with a G + C content of 54.2%, containing 276 putative protein-encoding genes and 24 tRNAs. A detailed comparative genomic analysis positioned vB_SmaM_Ps15 as a new species of the <i>Menderavirus</i> genus, which currently contains six very similar globally distributed members. It was confirmed as a virulent phage, free of known lysogenic and pathogenicity determinants, which supports its potential use for the treatment of <i>S. maltophilia</i> eye infections. |
| first_indexed | 2024-03-09T04:08:36Z |
| format | Article |
| id | doaj.art-4c9625e228ae4a258f09e41dd8970c44 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T04:08:36Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4c9625e228ae4a258f09e41dd8970c442023-12-03T14:03:10ZengMDPI AGViruses1999-49152022-03-0114470910.3390/v14040709Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to <i>Stenotrophomonas maltophilia</i> Clinical Ocular IsolatesDragica Damnjanović0Xabier Vázquez-Campos1Lisa Elliott2Mark Willcox3Wallace J. Bridge4School of Biotechnology and Biomolecular Sciences, The University of New South Wales, High Street, Kensington, NSW 2052, AustraliaNSW Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, The University of New South Wales, High Street, Kensington, NSW 2052, AustraliaAusPhage, 21 Everett Street, Mount St John, QLD 4818, AustraliaSchool of Optometry and Vision Science, The University of New South Wales, High Street, Kensington, NSW 2052, AustraliaSchool of Biotechnology and Biomolecular Sciences, The University of New South Wales, High Street, Kensington, NSW 2052, AustraliaRecent acknowledgment that multidrug resistant <i>Stenotrophomonas maltophilia</i> strains can cause severe infections has led to increasing global interest in addressing its pathogenicity. While being primarily associated with hospital-acquired respiratory tract infections, this bacterial species is also relevant to ophthalmology, particularly to contact lens-related diseases. In the current study, the capacity of <i>Stenotrophomonas</i> phage vB_SmaM_Ps15 to infect ocular <i>S. maltophilia</i> strains was investigated to explore its future potential as a phage therapeutic. The phage proved to be lytic to a range of clinical isolates collected in Australia from eye swabs, contact lenses and contact lens cases that had previously shown to be resistant to several antibiotics and multipurpose contact lenses disinfectant solutions. Morphological analysis by transmission electron microscopy placed the phage into the <i>Myoviridae</i> family. Its genome size was 161,350 bp with a G + C content of 54.2%, containing 276 putative protein-encoding genes and 24 tRNAs. A detailed comparative genomic analysis positioned vB_SmaM_Ps15 as a new species of the <i>Menderavirus</i> genus, which currently contains six very similar globally distributed members. It was confirmed as a virulent phage, free of known lysogenic and pathogenicity determinants, which supports its potential use for the treatment of <i>S. maltophilia</i> eye infections.https://www.mdpi.com/1999-4915/14/4/709<i>Stenotrophomonas maltophilia</i>bacteriophage<i>Menderavirus</i> |
| spellingShingle | Dragica Damnjanović Xabier Vázquez-Campos Lisa Elliott Mark Willcox Wallace J. Bridge Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to <i>Stenotrophomonas maltophilia</i> Clinical Ocular Isolates Viruses <i>Stenotrophomonas maltophilia</i> bacteriophage <i>Menderavirus</i> |
| title | Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to <i>Stenotrophomonas maltophilia</i> Clinical Ocular Isolates |
| title_full | Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to <i>Stenotrophomonas maltophilia</i> Clinical Ocular Isolates |
| title_fullStr | Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to <i>Stenotrophomonas maltophilia</i> Clinical Ocular Isolates |
| title_full_unstemmed | Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to <i>Stenotrophomonas maltophilia</i> Clinical Ocular Isolates |
| title_short | Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to <i>Stenotrophomonas maltophilia</i> Clinical Ocular Isolates |
| title_sort | characterisation of bacteriophage vb smam ps15 infective to i stenotrophomonas maltophilia i clinical ocular isolates |
| topic | <i>Stenotrophomonas maltophilia</i> bacteriophage <i>Menderavirus</i> |
| url | https://www.mdpi.com/1999-4915/14/4/709 |
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