Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate <em>Streptomyces</em> sp. SM17 When Compared to Its Terrestrial Relative <em>S. albidoflavus</em> J1074
Much recent interest has arisen in investigating <i>Streptomyces</i> isolates derived from the marine environment in the search for new bioactive compounds, particularly those found in association with marine invertebrates, such as sponges. Among these new compounds recently identified f...
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MDPI AG
2019-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/10/394 |
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| author | Eduardo L. Almeida Navdeep Kaur Laurence K. Jennings Andrés Felipe Carrillo Rincón Stephen A. Jackson Olivier P. Thomas Alan D.W. Dobson |
| author_facet | Eduardo L. Almeida Navdeep Kaur Laurence K. Jennings Andrés Felipe Carrillo Rincón Stephen A. Jackson Olivier P. Thomas Alan D.W. Dobson |
| author_sort | Eduardo L. Almeida |
| collection | DOAJ |
| description | Much recent interest has arisen in investigating <i>Streptomyces</i> isolates derived from the marine environment in the search for new bioactive compounds, particularly those found in association with marine invertebrates, such as sponges. Among these new compounds recently identified from marine <i>Streptomyces</i> isolates are the octapeptidic surugamides, which have been shown to possess anticancer and antifungal activities. By employing genome mining followed by an one strain many compounds (OSMAC)-based approach, we have identified the previously unreported capability of a marine sponge-derived isolate, namely <i>Streptomyces</i> sp. SM17, to produce surugamide A. Phylogenomics analyses provided novel insights on the distribution and conservation of the surugamides biosynthetic gene cluster (<i>sur</i> BGC) and suggested a closer relatedness between marine-derived <i>sur</i> BGCs than their terrestrially derived counterparts. Subsequent analysis showed differential production of surugamide A when comparing the closely related marine and terrestrial isolates, namely <i>Streptomyces</i> sp. SM17 and <i>Streptomyces albidoflavus</i> J1074. SM17 produced higher levels of surugamide A than <i>S. albidoflavus</i> J1074 under all conditions tested, and in particular producing >13-fold higher levels when grown in YD and 3-fold higher levels in SYP-NaCl medium. In addition, surugamide A production was repressed in TSB and YD medium, suggesting that carbon catabolite repression (CCR) may influence the production of surugamides in these strains. |
| first_indexed | 2024-12-11T08:25:19Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-11T08:25:19Z |
| publishDate | 2019-09-01 |
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| series | Microorganisms |
| spelling | doaj.art-4c96319b460c470ab5841d1212837ad22022-12-22T01:14:34ZengMDPI AGMicroorganisms2076-26072019-09-0171039410.3390/microorganisms7100394microorganisms7100394Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate <em>Streptomyces</em> sp. SM17 When Compared to Its Terrestrial Relative <em>S. albidoflavus</em> J1074Eduardo L. Almeida0Navdeep Kaur1Laurence K. Jennings2Andrés Felipe Carrillo Rincón3Stephen A. Jackson4Olivier P. Thomas5Alan D.W. Dobson6School of Microbiology, University College Cork, T12 YN60 Cork, IrelandMarine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway (NUI Galway), University Road, H91 TK33 Galway, IrelandMarine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway (NUI Galway), University Road, H91 TK33 Galway, IrelandSchool of Microbiology, University College Cork, T12 YN60 Cork, IrelandSchool of Microbiology, University College Cork, T12 YN60 Cork, IrelandMarine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway (NUI Galway), University Road, H91 TK33 Galway, IrelandSchool of Microbiology, University College Cork, T12 YN60 Cork, IrelandMuch recent interest has arisen in investigating <i>Streptomyces</i> isolates derived from the marine environment in the search for new bioactive compounds, particularly those found in association with marine invertebrates, such as sponges. Among these new compounds recently identified from marine <i>Streptomyces</i> isolates are the octapeptidic surugamides, which have been shown to possess anticancer and antifungal activities. By employing genome mining followed by an one strain many compounds (OSMAC)-based approach, we have identified the previously unreported capability of a marine sponge-derived isolate, namely <i>Streptomyces</i> sp. SM17, to produce surugamide A. Phylogenomics analyses provided novel insights on the distribution and conservation of the surugamides biosynthetic gene cluster (<i>sur</i> BGC) and suggested a closer relatedness between marine-derived <i>sur</i> BGCs than their terrestrially derived counterparts. Subsequent analysis showed differential production of surugamide A when comparing the closely related marine and terrestrial isolates, namely <i>Streptomyces</i> sp. SM17 and <i>Streptomyces albidoflavus</i> J1074. SM17 produced higher levels of surugamide A than <i>S. albidoflavus</i> J1074 under all conditions tested, and in particular producing >13-fold higher levels when grown in YD and 3-fold higher levels in SYP-NaCl medium. In addition, surugamide A production was repressed in TSB and YD medium, suggesting that carbon catabolite repression (CCR) may influence the production of surugamides in these strains.https://www.mdpi.com/2076-2607/7/10/394genome miningosmacphylogenomicssecondary metabolitessurugamidessurugamide amarine sponge-associated bacteria<i>streptomyces</i><i>albidoflavus</i> phylogroup |
| spellingShingle | Eduardo L. Almeida Navdeep Kaur Laurence K. Jennings Andrés Felipe Carrillo Rincón Stephen A. Jackson Olivier P. Thomas Alan D.W. Dobson Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate <em>Streptomyces</em> sp. SM17 When Compared to Its Terrestrial Relative <em>S. albidoflavus</em> J1074 Microorganisms genome mining osmac phylogenomics secondary metabolites surugamides surugamide a marine sponge-associated bacteria <i>streptomyces</i> <i>albidoflavus</i> phylogroup |
| title | Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate <em>Streptomyces</em> sp. SM17 When Compared to Its Terrestrial Relative <em>S. albidoflavus</em> J1074 |
| title_full | Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate <em>Streptomyces</em> sp. SM17 When Compared to Its Terrestrial Relative <em>S. albidoflavus</em> J1074 |
| title_fullStr | Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate <em>Streptomyces</em> sp. SM17 When Compared to Its Terrestrial Relative <em>S. albidoflavus</em> J1074 |
| title_full_unstemmed | Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate <em>Streptomyces</em> sp. SM17 When Compared to Its Terrestrial Relative <em>S. albidoflavus</em> J1074 |
| title_short | Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate <em>Streptomyces</em> sp. SM17 When Compared to Its Terrestrial Relative <em>S. albidoflavus</em> J1074 |
| title_sort | genome mining coupled with osmac based cultivation reveal differential production of surugamide a by the marine sponge isolate em streptomyces em sp sm17 when compared to its terrestrial relative em s albidoflavus em j1074 |
| topic | genome mining osmac phylogenomics secondary metabolites surugamides surugamide a marine sponge-associated bacteria <i>streptomyces</i> <i>albidoflavus</i> phylogroup |
| url | https://www.mdpi.com/2076-2607/7/10/394 |
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