Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds
Clinicopathological investigations are essential for the evaluation of the health status of ruminants. Apart from species-specific reference intervals, the effect of common biological factors should be considered for an accurate interpretation of laboratory data. The aim of this study was to evaluat...
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| Format: | Article |
| Language: | English |
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Sciendo
2019-12-01
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| Series: | Acta Veterinaria |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acve-2019-0037 |
| _version_ | 1819038366005460992 |
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| author | Oikonomidis Ioannis L. Kiossis Evangelos Brozos Christos Kritsepi-Konstantinou Maria |
| author_facet | Oikonomidis Ioannis L. Kiossis Evangelos Brozos Christos Kritsepi-Konstantinou Maria |
| author_sort | Oikonomidis Ioannis L. |
| collection | DOAJ |
| description | Clinicopathological investigations are essential for the evaluation of the health status of ruminants. Apart from species-specific reference intervals, the effect of common biological factors should be considered for an accurate interpretation of laboratory data. The aim of this study was to evaluate the effect of season on hematologic and biochemical analytes, and serum total thyroxine and cortisol in adult rams of two breeds. Four blood samples (one every season) were collected from each ram. Complete blood count was performed on the Advia 120 (Siemens Healthcare Diagnostics, USA), while the differential leukocyte count was manually conducted. Biochemical and hormonal analyses were performed on Flexor E (Vital Scientific, The Netherlands), AVL 9180 (Roche Diagnostics, Belgium), and Immulite 1000 (Siemens Healthcare Diagnostics, USA), respectively. Linear mixed effects models (R language) were employed for statistical analyses. Forty-three (26 Chios, 17 Florina), adult, clinically healthy rams were included. Statistically significant (p<0.05), mostly breed-independent seasonal differences were observed in almost all of the analytes. However, when assessing these differences in view of the respective reference intervals, only a few of them were considered biologically important. Specifically, mild hyperglycemia and mild decrease in the concentration of total calcium and inorganic phosphorus were detected in winter, while a mild increase in thyroxine concentration (autumn) and creatine kinase activity (spring and summer) was also noted. In conclusion, seasonal effects should be considered when evaluating laboratory results in rams; however, season does not appear to have an essential effect on the clinicopathological profile of rams reared in the Mediterranean region. |
| first_indexed | 2024-12-21T08:36:10Z |
| format | Article |
| id | doaj.art-4c99801c8583432f86b61fa51a38f5f0 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-12-21T08:36:10Z |
| publishDate | 2019-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-4c99801c8583432f86b61fa51a38f5f02022-12-21T19:10:03ZengSciendoActa Veterinaria1820-74482019-12-0169443444210.2478/acve-2019-0037Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two BreedsOikonomidis Ioannis L.0Kiossis Evangelos1Brozos Christos2Kritsepi-Konstantinou Maria3Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, GreeceFarm Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, GreeceFarm Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, GreeceDiagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, GreeceClinicopathological investigations are essential for the evaluation of the health status of ruminants. Apart from species-specific reference intervals, the effect of common biological factors should be considered for an accurate interpretation of laboratory data. The aim of this study was to evaluate the effect of season on hematologic and biochemical analytes, and serum total thyroxine and cortisol in adult rams of two breeds. Four blood samples (one every season) were collected from each ram. Complete blood count was performed on the Advia 120 (Siemens Healthcare Diagnostics, USA), while the differential leukocyte count was manually conducted. Biochemical and hormonal analyses were performed on Flexor E (Vital Scientific, The Netherlands), AVL 9180 (Roche Diagnostics, Belgium), and Immulite 1000 (Siemens Healthcare Diagnostics, USA), respectively. Linear mixed effects models (R language) were employed for statistical analyses. Forty-three (26 Chios, 17 Florina), adult, clinically healthy rams were included. Statistically significant (p<0.05), mostly breed-independent seasonal differences were observed in almost all of the analytes. However, when assessing these differences in view of the respective reference intervals, only a few of them were considered biologically important. Specifically, mild hyperglycemia and mild decrease in the concentration of total calcium and inorganic phosphorus were detected in winter, while a mild increase in thyroxine concentration (autumn) and creatine kinase activity (spring and summer) was also noted. In conclusion, seasonal effects should be considered when evaluating laboratory results in rams; however, season does not appear to have an essential effect on the clinicopathological profile of rams reared in the Mediterranean region.https://doi.org/10.2478/acve-2019-0037biochemistryendocrinologyhematologyreference intervalsheepvariation |
| spellingShingle | Oikonomidis Ioannis L. Kiossis Evangelos Brozos Christos Kritsepi-Konstantinou Maria Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds Acta Veterinaria biochemistry endocrinology hematology reference interval sheep variation |
| title | Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds |
| title_full | Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds |
| title_fullStr | Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds |
| title_full_unstemmed | Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds |
| title_short | Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds |
| title_sort | effect of season on hematologic biochemical and hormonal analytes in rams of two breeds |
| topic | biochemistry endocrinology hematology reference interval sheep variation |
| url | https://doi.org/10.2478/acve-2019-0037 |
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