Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy
The medicinal benefits of green seaweed Ulva have been documented in traditional Chinese medicine literatures. Sulfated polysaccharides found in Ulva are recognized as the primary bioactive compounds, known for their immunomodulatory and anti-inflammatory properties. Despite this knowledge, the avai...
| Үндсэн зохиолчид: | , , , |
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| Формат: | Өгүүллэг |
| Хэл сонгох: | English |
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Elsevier
2023-12-01
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| Цуврал: | Heliyon |
| Нөхцлүүд: | |
| Онлайн хандалт: | http://www.sciencedirect.com/science/article/pii/S240584402310048X |
| _version_ | 1827577456404463616 |
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| author | Jing-Yi Ou Yu-Jyun Wei Fang-Ling Liu Chung-Hsiung Huang |
| author_facet | Jing-Yi Ou Yu-Jyun Wei Fang-Ling Liu Chung-Hsiung Huang |
| author_sort | Jing-Yi Ou |
| collection | DOAJ |
| description | The medicinal benefits of green seaweed Ulva have been documented in traditional Chinese medicine literatures. Sulfated polysaccharides found in Ulva are recognized as the primary bioactive compounds, known for their immunomodulatory and anti-inflammatory properties. Despite this knowledge, the available information regarding anti-allergic activities of Ulva remains limited. The objective of this study was to prepare and characterize Ulva-derived polysaccharides (UP), oligosaccharides (UO), and residues (UR), followed by assessing their potential in improving allergic enteritis and gut microbiota in a murine model of ovalbumin (OVA)-induced food allergy. The immunomodulatory activities of UP, UO, and UR were evaluated by measuring the expression of serum antibodies, splenic cytokines and duodenal transcript factors of T cell subsets. The impact of UP, UO, and UR on enteric microbiota was explored by 16S rRNA gene sequencing analysis of fresh fecal samples from treated mice. Oral treatment of UP, UO, and UR noticeably attenuated allergic diarrhea and enteritis. Additionally, Ulva samples treatment decreased serum levels of IgG1 and OVA-specific IgE while increased the level of OVA-specific IgG. Enhanced production of IFN-γ and reduced production of IL-4 and IL-10 by splenocytes were observed in the treated mice. In parallel, Ulva samples treatment led to a decreased number of GATA3+ cells and an increased number of T-bet+ cells in the duodenum. However, the population of Foxp3+ cells was not significantly altered. Moreover, treatment of Ulva samples improved enteric dysbiosis evidenced by an increased abundance of Lactobacillus murinus, L. johnsonii, and L. reuteri, and a decreased abundance of Kineothrix alysoides, Lacrimispora saccharolytica, L. aerotolerans, and Erysipelotrichaceae in feces. In conclusion, UP, UO, and UR, which could modulate the Th1/Th2 immune balance, alleviate allergic enteritis and improve enteric dysbiosis in varying degrees, are potential to be developed into therapeutic agents for food allergy. |
| first_indexed | 2024-03-08T21:28:04Z |
| format | Article |
| id | doaj.art-4c9b8494473b4baebae3994cee8e4f59 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T21:28:04Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-4c9b8494473b4baebae3994cee8e4f592023-12-21T07:34:50ZengElsevierHeliyon2405-84402023-12-01912e22840Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergyJing-Yi Ou0Yu-Jyun Wei1Fang-Ling Liu2Chung-Hsiung Huang3Department of Food Science, National Taiwan Ocean University, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan; Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan; Corresponding author. Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.The medicinal benefits of green seaweed Ulva have been documented in traditional Chinese medicine literatures. Sulfated polysaccharides found in Ulva are recognized as the primary bioactive compounds, known for their immunomodulatory and anti-inflammatory properties. Despite this knowledge, the available information regarding anti-allergic activities of Ulva remains limited. The objective of this study was to prepare and characterize Ulva-derived polysaccharides (UP), oligosaccharides (UO), and residues (UR), followed by assessing their potential in improving allergic enteritis and gut microbiota in a murine model of ovalbumin (OVA)-induced food allergy. The immunomodulatory activities of UP, UO, and UR were evaluated by measuring the expression of serum antibodies, splenic cytokines and duodenal transcript factors of T cell subsets. The impact of UP, UO, and UR on enteric microbiota was explored by 16S rRNA gene sequencing analysis of fresh fecal samples from treated mice. Oral treatment of UP, UO, and UR noticeably attenuated allergic diarrhea and enteritis. Additionally, Ulva samples treatment decreased serum levels of IgG1 and OVA-specific IgE while increased the level of OVA-specific IgG. Enhanced production of IFN-γ and reduced production of IL-4 and IL-10 by splenocytes were observed in the treated mice. In parallel, Ulva samples treatment led to a decreased number of GATA3+ cells and an increased number of T-bet+ cells in the duodenum. However, the population of Foxp3+ cells was not significantly altered. Moreover, treatment of Ulva samples improved enteric dysbiosis evidenced by an increased abundance of Lactobacillus murinus, L. johnsonii, and L. reuteri, and a decreased abundance of Kineothrix alysoides, Lacrimispora saccharolytica, L. aerotolerans, and Erysipelotrichaceae in feces. In conclusion, UP, UO, and UR, which could modulate the Th1/Th2 immune balance, alleviate allergic enteritis and improve enteric dysbiosis in varying degrees, are potential to be developed into therapeutic agents for food allergy.http://www.sciencedirect.com/science/article/pii/S240584402310048XFood allergyDysbiosisOligosaccharidesPolysaccharidesResiduesUlva |
| spellingShingle | Jing-Yi Ou Yu-Jyun Wei Fang-Ling Liu Chung-Hsiung Huang Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy Heliyon Food allergy Dysbiosis Oligosaccharides Polysaccharides Residues Ulva |
| title | Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy |
| title_full | Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy |
| title_fullStr | Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy |
| title_full_unstemmed | Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy |
| title_short | Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy |
| title_sort | anti allergic effects of ulva derived polysaccharides oligosaccharides and residues in a murine model of food allergy |
| topic | Food allergy Dysbiosis Oligosaccharides Polysaccharides Residues Ulva |
| url | http://www.sciencedirect.com/science/article/pii/S240584402310048X |
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