Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity
Scope: House dust mite (HDM) induces Th2 responses in lungs and skin, but its effects in the intestine are poorly known. We aimed to study the involvement of HDM in the initial events that would promote sensitization through the oral route and eventually lead to allergy development. Methods and resu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/3/561 |
| _version_ | 1797542109733978112 |
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| author | Sara Benedé Leticia Pérez-Rodríguez Mónica Martínez-Blanco Elena Molina Rosina López-Fandiño |
| author_facet | Sara Benedé Leticia Pérez-Rodríguez Mónica Martínez-Blanco Elena Molina Rosina López-Fandiño |
| author_sort | Sara Benedé |
| collection | DOAJ |
| description | Scope: House dust mite (HDM) induces Th2 responses in lungs and skin, but its effects in the intestine are poorly known. We aimed to study the involvement of HDM in the initial events that would promote sensitization through the oral route and eventually lead to allergy development. Methods and results: BALB/c mice were exposed intragastrically to proteolytically active and inactive HDM, as such, or in combination with egg white (EW), and inflammatory and type 2 responses were evaluated. Oral administration of HDM, by virtue of its proteolytic activity, promoted the expression, in the small intestine, of genes encoding tight junction proteins, proinflammatory and Th2-biasing cytokines, and it caused expansion of group 2 innate immune cells, upregulation of Th2 cytokines, and dendritic cell migration and activation. In lymphoid tissues, its proteolytically inactivated counterpart also exerted an influence on the expression of surface DC molecules involved in interactions with T cells and in Th2 cell differentiation, which was confirmed in in vitro experiments. However, in our experimental setting we did not find evidence for the promotion of sensitization to coadministered EW. Conclusion: Orally administered HDM upregulates tissue damage factors and also acts as an activator of innate immune cells behaving similarly to potent oral Th2 adjuvants. |
| first_indexed | 2024-03-10T13:26:00Z |
| format | Article |
| id | doaj.art-4c9df4f63b2b48fc8323f975e46549a7 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T13:26:00Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-4c9df4f63b2b48fc8323f975e46549a72023-11-21T09:39:35ZengMDPI AGFoods2304-81582021-03-0110356110.3390/foods10030561Oral Exposure to House Dust Mite Activates Intestinal Innate ImmunitySara Benedé0Leticia Pérez-Rodríguez1Mónica Martínez-Blanco2Elena Molina3Rosina López-Fandiño4Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), 28049 Madrid, SpainInstituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), 28049 Madrid, SpainInstituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), 28049 Madrid, SpainInstituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), 28049 Madrid, SpainInstituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), 28049 Madrid, SpainScope: House dust mite (HDM) induces Th2 responses in lungs and skin, but its effects in the intestine are poorly known. We aimed to study the involvement of HDM in the initial events that would promote sensitization through the oral route and eventually lead to allergy development. Methods and results: BALB/c mice were exposed intragastrically to proteolytically active and inactive HDM, as such, or in combination with egg white (EW), and inflammatory and type 2 responses were evaluated. Oral administration of HDM, by virtue of its proteolytic activity, promoted the expression, in the small intestine, of genes encoding tight junction proteins, proinflammatory and Th2-biasing cytokines, and it caused expansion of group 2 innate immune cells, upregulation of Th2 cytokines, and dendritic cell migration and activation. In lymphoid tissues, its proteolytically inactivated counterpart also exerted an influence on the expression of surface DC molecules involved in interactions with T cells and in Th2 cell differentiation, which was confirmed in in vitro experiments. However, in our experimental setting we did not find evidence for the promotion of sensitization to coadministered EW. Conclusion: Orally administered HDM upregulates tissue damage factors and also acts as an activator of innate immune cells behaving similarly to potent oral Th2 adjuvants.https://www.mdpi.com/2304-8158/10/3/561food allergyhouse dust miteintestinal epithelial cellsdendritic cellsgroup 2 innate immune cells |
| spellingShingle | Sara Benedé Leticia Pérez-Rodríguez Mónica Martínez-Blanco Elena Molina Rosina López-Fandiño Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity Foods food allergy house dust mite intestinal epithelial cells dendritic cells group 2 innate immune cells |
| title | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
| title_full | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
| title_fullStr | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
| title_full_unstemmed | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
| title_short | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
| title_sort | oral exposure to house dust mite activates intestinal innate immunity |
| topic | food allergy house dust mite intestinal epithelial cells dendritic cells group 2 innate immune cells |
| url | https://www.mdpi.com/2304-8158/10/3/561 |
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