Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
CS5931 is a novel anticancer agent isolated from the sea squirt Ciona savignyi. However, its content in the species is very low, and developing a novel approach for production of the polypeptide is promising. In the present study, we expressed and purified the polypeptide from E. coli, and the ferme...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-03-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/14/3/47 |
| _version_ | 1828116915294306304 |
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| author | Xiaoshuang Chen Huanli Xu Bo Li Feng Wang Xiaoliang Chen Dexin Kong Xiukun Lin |
| author_facet | Xiaoshuang Chen Huanli Xu Bo Li Feng Wang Xiaoliang Chen Dexin Kong Xiukun Lin |
| author_sort | Xiaoshuang Chen |
| collection | DOAJ |
| description | CS5931 is a novel anticancer agent isolated from the sea squirt Ciona savignyi. However, its content in the species is very low, and developing a novel approach for production of the polypeptide is promising. In the present study, we expressed and purified the polypeptide from E. coli, and the fermentation conditions were studied using response surface methodology. The yield of CS5931 was increased from 2.0 to 7.5 mg/L. The denaturing and renaturation conditions were also studied. Using the optimized renaturation condition, the anticancer activity of refolding CS5931 was increased significantly; the value of IC50 was decreased from 23.2 to 11.6 μM. In vivo study using xenograft nude mice bearing HCT116 cancer cells revealed that CS5931 was able to inhibit the growth of tumor significantly. The study provides a useful approach for obtaining enough amount of CS5931 for further study. This study is also important for developing the polypeptide as a novel anticancer agent. |
| first_indexed | 2024-04-11T13:05:54Z |
| format | Article |
| id | doaj.art-4ca134d4d1ad48b4a4109d524fae1cf7 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:05:54Z |
| publishDate | 2016-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4ca134d4d1ad48b4a4109d524fae1cf72022-12-22T04:22:46ZengMDPI AGMarine Drugs1660-33972016-03-011434710.3390/md14030047md14030047Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona SavignyiXiaoshuang Chen0Huanli Xu1Bo Li2Feng Wang3Xiaoliang Chen4Dexin Kong5Xiukun Lin6School of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaDepartment of Pharmacolgoy, Capital Medical University, 10 Xitoutiao, Youanmen St., Beijing 100069, ChinaLinqu, Haifushan Hospital, Shandong 262600, ChinaLinqu, Haifushan Hospital, Shandong 262600, ChinaDepartment of Pharmacolgoy, Capital Medical University, 10 Xitoutiao, Youanmen St., Beijing 100069, ChinaSchool of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaDepartment of Pharmacolgoy, Capital Medical University, 10 Xitoutiao, Youanmen St., Beijing 100069, ChinaCS5931 is a novel anticancer agent isolated from the sea squirt Ciona savignyi. However, its content in the species is very low, and developing a novel approach for production of the polypeptide is promising. In the present study, we expressed and purified the polypeptide from E. coli, and the fermentation conditions were studied using response surface methodology. The yield of CS5931 was increased from 2.0 to 7.5 mg/L. The denaturing and renaturation conditions were also studied. Using the optimized renaturation condition, the anticancer activity of refolding CS5931 was increased significantly; the value of IC50 was decreased from 23.2 to 11.6 μM. In vivo study using xenograft nude mice bearing HCT116 cancer cells revealed that CS5931 was able to inhibit the growth of tumor significantly. The study provides a useful approach for obtaining enough amount of CS5931 for further study. This study is also important for developing the polypeptide as a novel anticancer agent.http://www.mdpi.com/1660-3397/14/3/47CS5931protein expressionrenaturationantitumor activity |
| spellingShingle | Xiaoshuang Chen Huanli Xu Bo Li Feng Wang Xiaoliang Chen Dexin Kong Xiukun Lin Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi Marine Drugs CS5931 protein expression renaturation antitumor activity |
| title | Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi |
| title_full | Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi |
| title_fullStr | Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi |
| title_full_unstemmed | Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi |
| title_short | Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi |
| title_sort | preparation and antitumor activity of cs5931 a novel polypeptide from sea squirt ciona savignyi |
| topic | CS5931 protein expression renaturation antitumor activity |
| url | http://www.mdpi.com/1660-3397/14/3/47 |
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