Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators
Abstract This report discusses the first demonstration of electrophoresis assisted time-of-flow mass spectrometry using ‘U’ shaped hollow nanomechanical resonators (HNR). Capillary electrophoresis was coupled with the HNR based mass detection to overcome low ionic conductivity of channels embedded i...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03846-y |
| _version_ | 1818752416084918272 |
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| author | Swathi Chaudhari Kamalesh Chaudhari Seokbeom Kim Faheem Khan Jungchul Lee Thomas Thundat |
| author_facet | Swathi Chaudhari Kamalesh Chaudhari Seokbeom Kim Faheem Khan Jungchul Lee Thomas Thundat |
| author_sort | Swathi Chaudhari |
| collection | DOAJ |
| description | Abstract This report discusses the first demonstration of electrophoresis assisted time-of-flow mass spectrometry using ‘U’ shaped hollow nanomechanical resonators (HNR). Capillary electrophoresis was coupled with the HNR based mass detection to overcome low ionic conductivity of channels embedded in the HNR preventing direct in-situ electrophoretic separation. The flow of analytes through the HNR was achieved by balancing the hydrodynamic pressure to override the electromotive force and inhibit the motion of analytes towards the anode for capillary electrophoresis. The resonance frequency shifts of the HNR vibrating around 1.5 MHz were correlated with the time of the passage of the protein bands to construct the mass spectrum. The proposed concept was demonstrated by constructing a mass spectrum of egg white proteins in the molecular weight range of 14–250 kDa. When compared to regular polyacrylamide gel electrophoresis, our method not only provides a precise and fast readout but also avoids the use of chemical staining. This study paves a new route for low-cost and on-chip mass spectrometers with ultra-miniaturized dimensions. |
| first_indexed | 2024-12-18T04:51:07Z |
| format | Article |
| id | doaj.art-4ca52c634fe446e0bc446f7bc824ea19 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:51:07Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4ca52c634fe446e0bc446f7bc824ea192022-12-21T21:20:24ZengNature PortfolioScientific Reports2045-23222017-06-01711710.1038/s41598-017-03846-yElectrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonatorsSwathi Chaudhari0Kamalesh Chaudhari1Seokbeom Kim2Faheem Khan3Jungchul Lee4Thomas Thundat5Department of Chemical and Materials Engineering, University of AlbertaDepartment of Chemical and Materials Engineering, University of AlbertaDepartment of Mechanical Engineering, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Sogang UniversityDepartment of Chemical and Materials Engineering, University of AlbertaDepartment of Mechanical Engineering, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Sogang UniversityDepartment of Chemical and Materials Engineering, University of AlbertaAbstract This report discusses the first demonstration of electrophoresis assisted time-of-flow mass spectrometry using ‘U’ shaped hollow nanomechanical resonators (HNR). Capillary electrophoresis was coupled with the HNR based mass detection to overcome low ionic conductivity of channels embedded in the HNR preventing direct in-situ electrophoretic separation. The flow of analytes through the HNR was achieved by balancing the hydrodynamic pressure to override the electromotive force and inhibit the motion of analytes towards the anode for capillary electrophoresis. The resonance frequency shifts of the HNR vibrating around 1.5 MHz were correlated with the time of the passage of the protein bands to construct the mass spectrum. The proposed concept was demonstrated by constructing a mass spectrum of egg white proteins in the molecular weight range of 14–250 kDa. When compared to regular polyacrylamide gel electrophoresis, our method not only provides a precise and fast readout but also avoids the use of chemical staining. This study paves a new route for low-cost and on-chip mass spectrometers with ultra-miniaturized dimensions.https://doi.org/10.1038/s41598-017-03846-y |
| spellingShingle | Swathi Chaudhari Kamalesh Chaudhari Seokbeom Kim Faheem Khan Jungchul Lee Thomas Thundat Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators Scientific Reports |
| title | Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators |
| title_full | Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators |
| title_fullStr | Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators |
| title_full_unstemmed | Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators |
| title_short | Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators |
| title_sort | electrophoresis assisted time of flow mass spectrometry using hollow nanomechanical resonators |
| url | https://doi.org/10.1038/s41598-017-03846-y |
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