Disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in Lutzomyia longipalpis females
Lutzomyia longipalpis is the most important vector of visceral leishmaniasis in Brazil. When female sandflies feed on blood, a peritrophic matrix (PM) is formed around the blood bolus. The PM is secreted by midgut cells and composed of proteins, glycoproteins and chitin microfibrils. The PM function...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2012-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000400016&lng=en&tlng=en |
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| author | Adriana Pereira Oliveira de Araújo Erich Loza Telleria Juliana da Matta Furniel Dutra Rute Maria Júlio Yara Maria Traub-Csekö |
| author_facet | Adriana Pereira Oliveira de Araújo Erich Loza Telleria Juliana da Matta Furniel Dutra Rute Maria Júlio Yara Maria Traub-Csekö |
| author_sort | Adriana Pereira Oliveira de Araújo |
| collection | DOAJ |
| description | Lutzomyia longipalpis is the most important vector of visceral leishmaniasis in Brazil. When female sandflies feed on blood, a peritrophic matrix (PM) is formed around the blood bolus. The PM is secreted by midgut cells and composed of proteins, glycoproteins and chitin microfibrils. The PM functions as both a physical barrier against pathogens present in the food bolus and blood meal digestion regulator. Previous studies of mosquitoes and sandflies have shown that the absence of a PM, resulting from adding an exogenous chitinase to the blood meal, accelerates digestion. In the present study, we analysed biological factors associated with the presence of a PM in L. longipalpis females. Insects fed blood containing chitinase (BCC) accelerated egg-laying relative to a control group fed blood without chitinase. However, in the BCC-fed insects, the number of females that died without laying eggs was higher and the number of eggs laid per female was lower. The eggs in both groups were viable and generated adults. Based on these data, we suggest that the absence of a PM accelerates nutrient acquisition, which results in premature egg production and oviposition; however, the absence of a PM reduces the total number of eggs laid per female. Reduced fecundity in the absence of a PM may be due to inefficient nutrient conversion or the loss of the protective role of the PM. |
| first_indexed | 2024-03-12T08:49:49Z |
| format | Article |
| id | doaj.art-4ca72844cbbb4ec3bbf57b5aa058e067 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:49:49Z |
| publishDate | 2012-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-4ca72844cbbb4ec3bbf57b5aa058e0672023-09-02T16:22:07ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602012-06-01107454354510.1590/S0074-02762012000400016S0074-02762012000400016Disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in Lutzomyia longipalpis femalesAdriana Pereira Oliveira de Araújo0Erich Loza Telleria1Juliana da Matta Furniel Dutra2Rute Maria Júlio3Yara Maria Traub-Csekö4Fundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzLutzomyia longipalpis is the most important vector of visceral leishmaniasis in Brazil. When female sandflies feed on blood, a peritrophic matrix (PM) is formed around the blood bolus. The PM is secreted by midgut cells and composed of proteins, glycoproteins and chitin microfibrils. The PM functions as both a physical barrier against pathogens present in the food bolus and blood meal digestion regulator. Previous studies of mosquitoes and sandflies have shown that the absence of a PM, resulting from adding an exogenous chitinase to the blood meal, accelerates digestion. In the present study, we analysed biological factors associated with the presence of a PM in L. longipalpis females. Insects fed blood containing chitinase (BCC) accelerated egg-laying relative to a control group fed blood without chitinase. However, in the BCC-fed insects, the number of females that died without laying eggs was higher and the number of eggs laid per female was lower. The eggs in both groups were viable and generated adults. Based on these data, we suggest that the absence of a PM accelerates nutrient acquisition, which results in premature egg production and oviposition; however, the absence of a PM reduces the total number of eggs laid per female. Reduced fecundity in the absence of a PM may be due to inefficient nutrient conversion or the loss of the protective role of the PM.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000400016&lng=en&tlng=enLutzomyia longipalpisperitrophic matrixchitinase |
| spellingShingle | Adriana Pereira Oliveira de Araújo Erich Loza Telleria Juliana da Matta Furniel Dutra Rute Maria Júlio Yara Maria Traub-Csekö Disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in Lutzomyia longipalpis females Memorias do Instituto Oswaldo Cruz Lutzomyia longipalpis peritrophic matrix chitinase |
| title | Disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in Lutzomyia longipalpis females |
| title_full | Disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in Lutzomyia longipalpis females |
| title_fullStr | Disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in Lutzomyia longipalpis females |
| title_full_unstemmed | Disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in Lutzomyia longipalpis females |
| title_short | Disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in Lutzomyia longipalpis females |
| title_sort | disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in lutzomyia longipalpis females |
| topic | Lutzomyia longipalpis peritrophic matrix chitinase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000400016&lng=en&tlng=en |
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