Excessive Gestational Weight Gain Alters DNA Methylation and Influences Foetal and Neonatal Body Composition
Background: Changes in body weight are associated with the regulation of DNA methylation (DNAm). In this study, we investigated the associations between maternal gestational weight gain-related DNAm and foetal and neonatal body composition. Methods: Brazilian pregnant women from the Araraquara Cohor...
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MDPI AG
2023-08-01
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| Series: | Epigenomes |
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| Online Access: | https://www.mdpi.com/2075-4655/7/3/18 |
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| author | Perla Pizzi Argentato João Victor da Silva Guerra Liania Alves Luzia Ester Silveira Ramos Mariana Maschietto Patrícia Helen de Carvalho Rondó |
| author_facet | Perla Pizzi Argentato João Victor da Silva Guerra Liania Alves Luzia Ester Silveira Ramos Mariana Maschietto Patrícia Helen de Carvalho Rondó |
| author_sort | Perla Pizzi Argentato |
| collection | DOAJ |
| description | Background: Changes in body weight are associated with the regulation of DNA methylation (DNAm). In this study, we investigated the associations between maternal gestational weight gain-related DNAm and foetal and neonatal body composition. Methods: Brazilian pregnant women from the Araraquara Cohort Study were followed up during pregnancy, delivery, and after hospital discharge. Women with normal pre-pregnancy BMI were allocated into two groups: adequate gestational weight gain (AGWG, <i>n</i> = 45) and excessive gestational weight gain (EGWG, <i>n</i> = 30). Foetal and neonatal body composition was evaluated via ultrasound and plethysmography, respectively. DNAm was assessed in maternal blood using Illumina Infinium MethylationEPIC BeadChip arrays. Linear regression models were used to explore the associations between DNAm and foetal and neonatal body composition. Results: Maternal weight, GWG, neonatal weight, and fat mass were higher in the EGWG group. Analysis of DNAm identified 46 differentially methylated positions and 11 differentially methylated regions (DMRs) between the EGWG and AGWG groups. Nine human phenotypes were enriched for these 11 DMRs located in 13 genes (<i>EMILIN1</i>, <i>HOXA5</i>, <i>CPT1B</i>, <i>CLDN9</i>, <i>ZFP57</i>, <i>BRCA1</i>, <i>POU5F1</i>, <i>ANKRD33</i>, <i>HLA-B</i>, <i>RANBP17</i>, <i>ZMYND11</i>, <i>DIP2C</i>, <i>TMEM232</i>), highlighting the terms insulin resistance, and hyperglycaemia. Maternal DNAm was associated with foetal total thigh and arm tissues and subcutaneous thigh and arm fat, as well as with neonatal fat mass percentage and fat mass. Conclusion: The methylation pattern in the EGWG group indicated a risk for developing chronic diseases and involvement of maternal DNAm in foetal lean and fat mass and in neonatal fat mass. |
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| issn | 2075-4655 |
| language | English |
| last_indexed | 2024-03-10T22:46:12Z |
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| spelling | doaj.art-4ca73e3f84bb40dfa66b0c870dc91edb2023-11-19T10:38:21ZengMDPI AGEpigenomes2075-46552023-08-01731810.3390/epigenomes7030018Excessive Gestational Weight Gain Alters DNA Methylation and Influences Foetal and Neonatal Body CompositionPerla Pizzi Argentato0João Victor da Silva Guerra1Liania Alves Luzia2Ester Silveira Ramos3Mariana Maschietto4Patrícia Helen de Carvalho Rondó5Nutrition Department, School of Public Health, University of São Paulo, Avenida Dr. Arnaldo 715, São Paulo 01246-904, SP, BrazilBrazilian Biosciences National Laboratory (LNBio), Brazilian Centre for Research in Energy and Materials (CNPEM) and Graduate Program in Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, University of Campinas, Rua Giuseppe Máximo Scolfaro 10.000, Cidade Universitária, Campinas 13083-970, SP, BrazilNutrition Department, School of Public Health, University of São Paulo, Avenida Dr. Arnaldo 715, São Paulo 01246-904, SP, BrazilDepartment of Genetics, Ribeirão Preto Medical School, University of São Paulo, Avenida Bandeirantes 3900, Ribeirão Preto 14049-900, SP, BrazilBoldrini Children’s Hospital, University of Campinas, Rua Márcia Mendes 619, Cidade Universitária, Campinas 13083-884, SP, BrazilNutrition Department, School of Public Health, University of São Paulo, Avenida Dr. Arnaldo 715, São Paulo 01246-904, SP, BrazilBackground: Changes in body weight are associated with the regulation of DNA methylation (DNAm). In this study, we investigated the associations between maternal gestational weight gain-related DNAm and foetal and neonatal body composition. Methods: Brazilian pregnant women from the Araraquara Cohort Study were followed up during pregnancy, delivery, and after hospital discharge. Women with normal pre-pregnancy BMI were allocated into two groups: adequate gestational weight gain (AGWG, <i>n</i> = 45) and excessive gestational weight gain (EGWG, <i>n</i> = 30). Foetal and neonatal body composition was evaluated via ultrasound and plethysmography, respectively. DNAm was assessed in maternal blood using Illumina Infinium MethylationEPIC BeadChip arrays. Linear regression models were used to explore the associations between DNAm and foetal and neonatal body composition. Results: Maternal weight, GWG, neonatal weight, and fat mass were higher in the EGWG group. Analysis of DNAm identified 46 differentially methylated positions and 11 differentially methylated regions (DMRs) between the EGWG and AGWG groups. Nine human phenotypes were enriched for these 11 DMRs located in 13 genes (<i>EMILIN1</i>, <i>HOXA5</i>, <i>CPT1B</i>, <i>CLDN9</i>, <i>ZFP57</i>, <i>BRCA1</i>, <i>POU5F1</i>, <i>ANKRD33</i>, <i>HLA-B</i>, <i>RANBP17</i>, <i>ZMYND11</i>, <i>DIP2C</i>, <i>TMEM232</i>), highlighting the terms insulin resistance, and hyperglycaemia. Maternal DNAm was associated with foetal total thigh and arm tissues and subcutaneous thigh and arm fat, as well as with neonatal fat mass percentage and fat mass. Conclusion: The methylation pattern in the EGWG group indicated a risk for developing chronic diseases and involvement of maternal DNAm in foetal lean and fat mass and in neonatal fat mass.https://www.mdpi.com/2075-4655/7/3/18gestational weight gainDNA methylationultrasonographyplethysmographyoffspring body composition |
| spellingShingle | Perla Pizzi Argentato João Victor da Silva Guerra Liania Alves Luzia Ester Silveira Ramos Mariana Maschietto Patrícia Helen de Carvalho Rondó Excessive Gestational Weight Gain Alters DNA Methylation and Influences Foetal and Neonatal Body Composition Epigenomes gestational weight gain DNA methylation ultrasonography plethysmography offspring body composition |
| title | Excessive Gestational Weight Gain Alters DNA Methylation and Influences Foetal and Neonatal Body Composition |
| title_full | Excessive Gestational Weight Gain Alters DNA Methylation and Influences Foetal and Neonatal Body Composition |
| title_fullStr | Excessive Gestational Weight Gain Alters DNA Methylation and Influences Foetal and Neonatal Body Composition |
| title_full_unstemmed | Excessive Gestational Weight Gain Alters DNA Methylation and Influences Foetal and Neonatal Body Composition |
| title_short | Excessive Gestational Weight Gain Alters DNA Methylation and Influences Foetal and Neonatal Body Composition |
| title_sort | excessive gestational weight gain alters dna methylation and influences foetal and neonatal body composition |
| topic | gestational weight gain DNA methylation ultrasonography plethysmography offspring body composition |
| url | https://www.mdpi.com/2075-4655/7/3/18 |
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