DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis.
RNA-directed DNA methylation (RdDM) and histone H3 lysine 9 dimethylation (H3K9me2) are related transcriptional silencing mechanisms that target transposable elements (TEs) and repeats to maintain genome stability in plants. RdDM is mediated by small and long noncoding RNAs produced by the plant-spe...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-07-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC4080997?pdf=render |
| _version_ | 1819077232734240768 |
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| author | Thanh Theresa Dinh Lei Gao Xigang Liu Dongming Li Shengben Li Yuanyuan Zhao Michael O'Leary Brandon Le Robert J Schmitz Pablo A Manavella Shaofang Li Detlef Weigel Olga Pontes Joseph R Ecker Xuemei Chen |
| author_facet | Thanh Theresa Dinh Lei Gao Xigang Liu Dongming Li Shengben Li Yuanyuan Zhao Michael O'Leary Brandon Le Robert J Schmitz Pablo A Manavella Shaofang Li Detlef Weigel Olga Pontes Joseph R Ecker Xuemei Chen |
| author_sort | Thanh Theresa Dinh |
| collection | DOAJ |
| description | RNA-directed DNA methylation (RdDM) and histone H3 lysine 9 dimethylation (H3K9me2) are related transcriptional silencing mechanisms that target transposable elements (TEs) and repeats to maintain genome stability in plants. RdDM is mediated by small and long noncoding RNAs produced by the plant-specific RNA polymerases Pol IV and Pol V, respectively. Through a chemical genetics screen with a luciferase-based DNA methylation reporter, LUCL, we found that camptothecin, a compound with anti-cancer properties that targets DNA topoisomerase 1α (TOP1α) was able to de-repress LUCL by reducing its DNA methylation and H3K9me2 levels. Further studies with Arabidopsis top1α mutants showed that TOP1α silences endogenous RdDM loci by facilitating the production of Pol V-dependent long non-coding RNAs, AGONAUTE4 recruitment and H3K9me2 deposition at TEs and repeats. This study assigned a new role in epigenetic silencing to an enzyme that affects DNA topology. |
| first_indexed | 2024-12-21T18:53:56Z |
| format | Article |
| id | doaj.art-4caa2cf0d62b4b0db8ecd94b4b82be11 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-21T18:53:56Z |
| publishDate | 2014-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-4caa2cf0d62b4b0db8ecd94b4b82be112022-12-21T18:53:40ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042014-07-01107e100444610.1371/journal.pgen.1004446DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis.Thanh Theresa DinhLei GaoXigang LiuDongming LiShengben LiYuanyuan ZhaoMichael O'LearyBrandon LeRobert J SchmitzPablo A ManavellaShaofang LiDetlef WeigelOlga PontesJoseph R EckerXuemei ChenRNA-directed DNA methylation (RdDM) and histone H3 lysine 9 dimethylation (H3K9me2) are related transcriptional silencing mechanisms that target transposable elements (TEs) and repeats to maintain genome stability in plants. RdDM is mediated by small and long noncoding RNAs produced by the plant-specific RNA polymerases Pol IV and Pol V, respectively. Through a chemical genetics screen with a luciferase-based DNA methylation reporter, LUCL, we found that camptothecin, a compound with anti-cancer properties that targets DNA topoisomerase 1α (TOP1α) was able to de-repress LUCL by reducing its DNA methylation and H3K9me2 levels. Further studies with Arabidopsis top1α mutants showed that TOP1α silences endogenous RdDM loci by facilitating the production of Pol V-dependent long non-coding RNAs, AGONAUTE4 recruitment and H3K9me2 deposition at TEs and repeats. This study assigned a new role in epigenetic silencing to an enzyme that affects DNA topology.http://europepmc.org/articles/PMC4080997?pdf=render |
| spellingShingle | Thanh Theresa Dinh Lei Gao Xigang Liu Dongming Li Shengben Li Yuanyuan Zhao Michael O'Leary Brandon Le Robert J Schmitz Pablo A Manavella Shaofang Li Detlef Weigel Olga Pontes Joseph R Ecker Xuemei Chen DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis. PLoS Genetics |
| title | DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis. |
| title_full | DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis. |
| title_fullStr | DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis. |
| title_full_unstemmed | DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis. |
| title_short | DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis. |
| title_sort | dna topoisomerase 1α promotes transcriptional silencing of transposable elements through dna methylation and histone lysine 9 dimethylation in arabidopsis |
| url | http://europepmc.org/articles/PMC4080997?pdf=render |
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