Metagenomic analysis of Raphidiopsis raciborskii microbiome: beyond the individual
Raphidiopsis raciborskii is a toxic, invasive bacteria with a defined biogeographic pattern attributed to the generation of ecotypes subjected to local environmental filters and to phenotypic plasticity. The interactions taking place between the cyanobacterium and the other bacteria inhabiting the e...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2021-10-01
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| Series: | Biodiversity Data Journal |
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| Online Access: | https://bdj.pensoft.net/article/72514/download/pdf/ |
| _version_ | 1818754989992968192 |
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| author | Paula Vico Andrés Iriarte Sylvia Bonilla Claudia Piccini |
| author_facet | Paula Vico Andrés Iriarte Sylvia Bonilla Claudia Piccini |
| author_sort | Paula Vico |
| collection | DOAJ |
| description | Raphidiopsis raciborskii is a toxic, invasive bacteria with a defined biogeographic pattern attributed to the generation of ecotypes subjected to local environmental filters and to phenotypic plasticity. The interactions taking place between the cyanobacterium and the other bacteria inhabiting the external polysaccharide-rich matrix surrounding the cells, or phycosphere, may be ecotype-specific and would have different influence on the carbon and nutrient cycling in the ecosystem. Here, we describe the bacterial community or microbiome (assessed by 16S rRNA metagenomics) associated to two R. raciborskii strains that have been described as different ecotypes: the saxitoxin-producer MVCC19 and the non-toxic LB2897. Our results showed that both ecotypes share 50% of their microbiomes and differ in their dominant taxa. The taxon having the highest abundance in the microbiome of MVCC19 was Neorhizobium (22.5% relative abundance), while the dominant taxon in LB2897 was the Planctomycetes SM1A02 (26.2% relative abundance). These groups exhibit different metabolic capabilities regarding nitrogen acquisition (symbiotic nitrogen-fixing in Neorhizobium vs. anammox in SM1A02), suggesting the existence of ecotype-specific microbiomes that play a relevant role in cyanobacterial niche-adaptation. In addition, as saxitoxin and analogues are nitrogen-rich (7 atoms per molecule), we hypothesise that saxitoxin-producing R. raciborskii benefits from external sources of nitrogen provided by the microbiome bacteria. Based on these findings, we propose that the mechanisms involved in the assembly of the cyanobacterial microbiome community are ecotype-dependent. |
| first_indexed | 2024-12-18T05:32:01Z |
| format | Article |
| id | doaj.art-4caaf7d1f05c4a1aab9429cfd056ae0b |
| institution | Directory Open Access Journal |
| issn | 1314-2828 |
| language | English |
| last_indexed | 2024-12-18T05:32:01Z |
| publishDate | 2021-10-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Biodiversity Data Journal |
| spelling | doaj.art-4caaf7d1f05c4a1aab9429cfd056ae0b2022-12-21T21:19:25ZengPensoft PublishersBiodiversity Data Journal1314-28282021-10-01911910.3897/BDJ.9.e7251472514Metagenomic analysis of Raphidiopsis raciborskii microbiome: beyond the individualPaula Vico0Andrés Iriarte1Sylvia Bonilla2Claudia Piccini3Instituto de Investigaciones Biológicas Clemente EstableInstituto de Higiene, Facultad de Medicina, UDELARSección Limnología. Facultad de Ciencias, UDELARInstituto de Investigaciones Biológicas Clemente EstableRaphidiopsis raciborskii is a toxic, invasive bacteria with a defined biogeographic pattern attributed to the generation of ecotypes subjected to local environmental filters and to phenotypic plasticity. The interactions taking place between the cyanobacterium and the other bacteria inhabiting the external polysaccharide-rich matrix surrounding the cells, or phycosphere, may be ecotype-specific and would have different influence on the carbon and nutrient cycling in the ecosystem. Here, we describe the bacterial community or microbiome (assessed by 16S rRNA metagenomics) associated to two R. raciborskii strains that have been described as different ecotypes: the saxitoxin-producer MVCC19 and the non-toxic LB2897. Our results showed that both ecotypes share 50% of their microbiomes and differ in their dominant taxa. The taxon having the highest abundance in the microbiome of MVCC19 was Neorhizobium (22.5% relative abundance), while the dominant taxon in LB2897 was the Planctomycetes SM1A02 (26.2% relative abundance). These groups exhibit different metabolic capabilities regarding nitrogen acquisition (symbiotic nitrogen-fixing in Neorhizobium vs. anammox in SM1A02), suggesting the existence of ecotype-specific microbiomes that play a relevant role in cyanobacterial niche-adaptation. In addition, as saxitoxin and analogues are nitrogen-rich (7 atoms per molecule), we hypothesise that saxitoxin-producing R. raciborskii benefits from external sources of nitrogen provided by the microbiome bacteria. Based on these findings, we propose that the mechanisms involved in the assembly of the cyanobacterial microbiome community are ecotype-dependent.https://bdj.pensoft.net/article/72514/download/pdf/<i>Raphidiopsis raciborskii</i>microbiome16S r |
| spellingShingle | Paula Vico Andrés Iriarte Sylvia Bonilla Claudia Piccini Metagenomic analysis of Raphidiopsis raciborskii microbiome: beyond the individual Biodiversity Data Journal <i>Raphidiopsis raciborskii</i> microbiome 16S r |
| title | Metagenomic analysis of Raphidiopsis raciborskii microbiome: beyond the individual |
| title_full | Metagenomic analysis of Raphidiopsis raciborskii microbiome: beyond the individual |
| title_fullStr | Metagenomic analysis of Raphidiopsis raciborskii microbiome: beyond the individual |
| title_full_unstemmed | Metagenomic analysis of Raphidiopsis raciborskii microbiome: beyond the individual |
| title_short | Metagenomic analysis of Raphidiopsis raciborskii microbiome: beyond the individual |
| title_sort | metagenomic analysis of raphidiopsis raciborskii microbiome beyond the individual |
| topic | <i>Raphidiopsis raciborskii</i> microbiome 16S r |
| url | https://bdj.pensoft.net/article/72514/download/pdf/ |
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