Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection
Resection of the olfactory mucosa (OM) is sometimes unavoidable during surgery; however, it is not known whether the OM can completely recover thereafter. The aim of this study was to uncover whether the OM fully recovers after mucosal resection and describe the process of OM regeneration. 8-week-ol...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-07-01
|
| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2021.695653/full |
| _version_ | 1831546036986839040 |
|---|---|
| author | Eri Mori Rumi Ueha Rumi Ueha Kenji Kondo Shotaro Funada Hajime Shimmura Kai Kanemoto Hirotaka Tanaka Hironobu Nishijima Nobuyoshi Otori Tatsuya Yamasoba Hiromi Kojima |
| author_facet | Eri Mori Rumi Ueha Rumi Ueha Kenji Kondo Shotaro Funada Hajime Shimmura Kai Kanemoto Hirotaka Tanaka Hironobu Nishijima Nobuyoshi Otori Tatsuya Yamasoba Hiromi Kojima |
| author_sort | Eri Mori |
| collection | DOAJ |
| description | Resection of the olfactory mucosa (OM) is sometimes unavoidable during surgery; however, it is not known whether the OM can completely recover thereafter. The aim of this study was to uncover whether the OM fully recovers after mucosal resection and describe the process of OM regeneration. 8-week-old male Sprague–Dawley rats (n = 18) were subjected to OM resection at the nasal septum; six rats were euthanized for histological examination 0, 30, and 90 days after surgery. Immunohistochemistry was performed to identify olfactory receptor neuron (ORN) lineage cells [mature and immature ORNs and ORN progenitors, and olfactory ensheathing cells (OECs)], as well as dividing and apoptotic cells. Squamous and respiratory metaplasia and inflammatory cell infiltration were also assessed. On day 30 after resection, the mucosa had regenerated, and mainly contained thin nerve bundles, basal cells, and immature ORNs, with a few mature ORNs and OECs. On day 90, the repaired nasal mucosa had degenerated into stratified squamous or ciliated pseudostratified columnar epithelia, with reducing ORNs. The lamina propria contained numerous macrophages. Partial regeneration was observed within 1 month after OM resection, whereas subsequent degeneration into squamous and respiratory epithelia occurred within 3 months. Given the poor persistence of ORNs and OECs, OM resection is likely to result in olfactory impairment. Overall, surgeons should be cautious not to injure the OM during surgery. |
| first_indexed | 2024-12-17T01:37:31Z |
| format | Article |
| id | doaj.art-4cacbfb75b4546afb9d0df6544372e3b |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-17T01:37:31Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-4cacbfb75b4546afb9d0df6544372e3b2022-12-21T22:08:25ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2021-07-011510.3389/fnins.2021.695653695653Squamous and Respiratory Metaplasia After Olfactory Mucosal ResectionEri Mori0Rumi Ueha1Rumi Ueha2Kenji Kondo3Shotaro Funada4Hajime Shimmura5Kai Kanemoto6Hirotaka Tanaka7Hironobu Nishijima8Nobuyoshi Otori9Tatsuya Yamasoba10Hiromi Kojima11Department of Otorhinolaryngology, The Jikei University School of Medicine, Tokyo, JapanSwallowing Center, The University of Tokyo Hospital, Tokyo, JapanDepartment of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDepartment of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDepartment of Otorhinolaryngology, The Jikei University School of Medicine, Tokyo, JapanDepartment of Otorhinolaryngology, The Jikei University School of Medicine, Tokyo, JapanDepartment of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDepartment of Otorhinolaryngology, The Jikei University School of Medicine, Tokyo, JapanDepartment of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDepartment of Otorhinolaryngology, The Jikei University School of Medicine, Tokyo, JapanDepartment of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, Tokyo, JapanDepartment of Otorhinolaryngology, The Jikei University School of Medicine, Tokyo, JapanResection of the olfactory mucosa (OM) is sometimes unavoidable during surgery; however, it is not known whether the OM can completely recover thereafter. The aim of this study was to uncover whether the OM fully recovers after mucosal resection and describe the process of OM regeneration. 8-week-old male Sprague–Dawley rats (n = 18) were subjected to OM resection at the nasal septum; six rats were euthanized for histological examination 0, 30, and 90 days after surgery. Immunohistochemistry was performed to identify olfactory receptor neuron (ORN) lineage cells [mature and immature ORNs and ORN progenitors, and olfactory ensheathing cells (OECs)], as well as dividing and apoptotic cells. Squamous and respiratory metaplasia and inflammatory cell infiltration were also assessed. On day 30 after resection, the mucosa had regenerated, and mainly contained thin nerve bundles, basal cells, and immature ORNs, with a few mature ORNs and OECs. On day 90, the repaired nasal mucosa had degenerated into stratified squamous or ciliated pseudostratified columnar epithelia, with reducing ORNs. The lamina propria contained numerous macrophages. Partial regeneration was observed within 1 month after OM resection, whereas subsequent degeneration into squamous and respiratory epithelia occurred within 3 months. Given the poor persistence of ORNs and OECs, OM resection is likely to result in olfactory impairment. Overall, surgeons should be cautious not to injure the OM during surgery.https://www.frontiersin.org/articles/10.3389/fnins.2021.695653/fullolfactory mucosaolfactory receptor neuronssmellolfactory impairmentolfactory ensheathing cells |
| spellingShingle | Eri Mori Rumi Ueha Rumi Ueha Kenji Kondo Shotaro Funada Hajime Shimmura Kai Kanemoto Hirotaka Tanaka Hironobu Nishijima Nobuyoshi Otori Tatsuya Yamasoba Hiromi Kojima Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection Frontiers in Neuroscience olfactory mucosa olfactory receptor neurons smell olfactory impairment olfactory ensheathing cells |
| title | Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection |
| title_full | Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection |
| title_fullStr | Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection |
| title_full_unstemmed | Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection |
| title_short | Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection |
| title_sort | squamous and respiratory metaplasia after olfactory mucosal resection |
| topic | olfactory mucosa olfactory receptor neurons smell olfactory impairment olfactory ensheathing cells |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2021.695653/full |
| work_keys_str_mv | AT erimori squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT rumiueha squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT rumiueha squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT kenjikondo squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT shotarofunada squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT hajimeshimmura squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT kaikanemoto squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT hirotakatanaka squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT hironobunishijima squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT nobuyoshiotori squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT tatsuyayamasoba squamousandrespiratorymetaplasiaafterolfactorymucosalresection AT hiromikojima squamousandrespiratorymetaplasiaafterolfactorymucosalresection |