Genome-Wide Identification of the <i>Nramp</i> Gene Family in <i>Spirodela polyrhiza</i> and Expression Analysis under Cadmium Stress
Natural resistance-associated macrophage proteins (Nramps) are specific metal transporters in plants with different functions among various species. The evolutionary and functional information of the <i>Nramp</i> gene family in <i>Spirodela polyrhiza</i> has not been previous...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6414 |
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| author | Yan Chen Xuyao Zhao Gaojie Li Sunjeet Kumar Zuoliang Sun Yixian Li Wenjun Guo Jingjing Yang Hongwei Hou |
| author_facet | Yan Chen Xuyao Zhao Gaojie Li Sunjeet Kumar Zuoliang Sun Yixian Li Wenjun Guo Jingjing Yang Hongwei Hou |
| author_sort | Yan Chen |
| collection | DOAJ |
| description | Natural resistance-associated macrophage proteins (Nramps) are specific metal transporters in plants with different functions among various species. The evolutionary and functional information of the <i>Nramp</i> gene family in <i>Spirodela polyrhiza</i> has not been previously reported in detail. To identify the <i>Nramp</i> genes in <i>S. polyrhiza</i>, we performed genome-wide identification, characterization, classification, and cis-elements analysis among 22 species with 138 amino acid sequences. We also conducted chromosomal localization and analyzed the synteny relationship, promoter, subcellular localization, and expression patterns in <i>S. polyrhiza</i>. β-Glucuronidase staining indicated that <i>SpNramp</i><i>1</i> and <i>SpNramp3</i> mainly accumulated in the root and joint between mother and daughter frond. Moreover, <i>SpNramp1</i> was also widely displayed in the frond. <i>SpNramp2</i> was intensively distributed in the root and frond. Quantitative real-time PCR results proved that the <i>SpNramp</i> gene expression level was influenced by Cd stress, especially in response to Fe or Mn deficiency. The study provides detailed information on the <i>SpNramp</i> gene family and their distribution and expression, laying a beneficial foundation for functional research. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:23:23Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4cace470d41246a69991c50d31ae1fea2023-11-22T00:14:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212641410.3390/ijms22126414Genome-Wide Identification of the <i>Nramp</i> Gene Family in <i>Spirodela polyrhiza</i> and Expression Analysis under Cadmium StressYan Chen0Xuyao Zhao1Gaojie Li2Sunjeet Kumar3Zuoliang Sun4Yixian Li5Wenjun Guo6Jingjing Yang7Hongwei Hou8The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaNatural resistance-associated macrophage proteins (Nramps) are specific metal transporters in plants with different functions among various species. The evolutionary and functional information of the <i>Nramp</i> gene family in <i>Spirodela polyrhiza</i> has not been previously reported in detail. To identify the <i>Nramp</i> genes in <i>S. polyrhiza</i>, we performed genome-wide identification, characterization, classification, and cis-elements analysis among 22 species with 138 amino acid sequences. We also conducted chromosomal localization and analyzed the synteny relationship, promoter, subcellular localization, and expression patterns in <i>S. polyrhiza</i>. β-Glucuronidase staining indicated that <i>SpNramp</i><i>1</i> and <i>SpNramp3</i> mainly accumulated in the root and joint between mother and daughter frond. Moreover, <i>SpNramp1</i> was also widely displayed in the frond. <i>SpNramp2</i> was intensively distributed in the root and frond. Quantitative real-time PCR results proved that the <i>SpNramp</i> gene expression level was influenced by Cd stress, especially in response to Fe or Mn deficiency. The study provides detailed information on the <i>SpNramp</i> gene family and their distribution and expression, laying a beneficial foundation for functional research.https://www.mdpi.com/1422-0067/22/12/6414<i>Spirodela polyrhiza</i><i>Nramp</i> familypromoterCdFe or Mn limitinggene expression profiling |
| spellingShingle | Yan Chen Xuyao Zhao Gaojie Li Sunjeet Kumar Zuoliang Sun Yixian Li Wenjun Guo Jingjing Yang Hongwei Hou Genome-Wide Identification of the <i>Nramp</i> Gene Family in <i>Spirodela polyrhiza</i> and Expression Analysis under Cadmium Stress International Journal of Molecular Sciences <i>Spirodela polyrhiza</i> <i>Nramp</i> family promoter Cd Fe or Mn limiting gene expression profiling |
| title | Genome-Wide Identification of the <i>Nramp</i> Gene Family in <i>Spirodela polyrhiza</i> and Expression Analysis under Cadmium Stress |
| title_full | Genome-Wide Identification of the <i>Nramp</i> Gene Family in <i>Spirodela polyrhiza</i> and Expression Analysis under Cadmium Stress |
| title_fullStr | Genome-Wide Identification of the <i>Nramp</i> Gene Family in <i>Spirodela polyrhiza</i> and Expression Analysis under Cadmium Stress |
| title_full_unstemmed | Genome-Wide Identification of the <i>Nramp</i> Gene Family in <i>Spirodela polyrhiza</i> and Expression Analysis under Cadmium Stress |
| title_short | Genome-Wide Identification of the <i>Nramp</i> Gene Family in <i>Spirodela polyrhiza</i> and Expression Analysis under Cadmium Stress |
| title_sort | genome wide identification of the i nramp i gene family in i spirodela polyrhiza i and expression analysis under cadmium stress |
| topic | <i>Spirodela polyrhiza</i> <i>Nramp</i> family promoter Cd Fe or Mn limiting gene expression profiling |
| url | https://www.mdpi.com/1422-0067/22/12/6414 |
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