Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, China
IntroductionThe widespread use of antibiotics in animal agriculture has increased the resistance of Escherichia coli, and pathogenic E. coli often harbor complex virulence factors. Antimicrobial resistance in pathogenic bacteria can cause public health problems. Correlation analyses of the resistanc...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-06-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1202013/full |
| _version_ | 1797803789056475136 |
|---|---|
| author | Shuaishuai Luo Cuiyi Liao Cuiyi Liao Jinju Peng Songruo Tao Tengyue Zhang Yue Dai Yuexia Ding Yi Ma Yi Ma |
| author_facet | Shuaishuai Luo Cuiyi Liao Cuiyi Liao Jinju Peng Songruo Tao Tengyue Zhang Yue Dai Yuexia Ding Yi Ma Yi Ma |
| author_sort | Shuaishuai Luo |
| collection | DOAJ |
| description | IntroductionThe widespread use of antibiotics in animal agriculture has increased the resistance of Escherichia coli, and pathogenic E. coli often harbor complex virulence factors. Antimicrobial resistance in pathogenic bacteria can cause public health problems. Correlation analyses of the resistance, virulence, and serotype data from the pathogenic bacteria found on farms and in the surrounding environment can thus provide extremely valuable data to help improve public health management.MethodsIn this investigation, we have assessed the drug resistance and virulence genes as well as the molecular typing characteristics of 30 E. coli strains isolated from duck farms in the Zhanjiang area of China. Polymerase chain reaction was used to detect the drug resistance and virulence genes as well as serotypes, and whole-genome sequencing was used to analyze the multilocus sequence typing.ResultsThe detection rates for the oqxA resistance gene and fimC virulence gene were highest (93.3%, respectively). There were no correlations between the drug resistance and virulence gene numbers in the same strain. The epidemic serotype was O81 (5/24), ST3856 was an epidemic sequence type, and strains I-9 and III-6 carried 11 virulence genes. The E. coli strains from the duck farms in the Zhanjiang area were thus found to have a broad drug resistance spectrum, various virulence genes, complex serotypes, and certain pathogenicity and genetic relationship.DiscussionMonitoring the spread of pathogenic bacteria and the provision of guidance regarding the use of antibiotics in the livestock and poultry industries will be required in the future in the Zhanjiang area. |
| first_indexed | 2024-03-13T05:27:17Z |
| format | Article |
| id | doaj.art-4cad2362235e40b6815bc1b54f597217 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-13T05:27:17Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-4cad2362235e40b6815bc1b54f5972172023-06-15T05:06:02ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-06-011310.3389/fcimb.2023.12020131202013Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, ChinaShuaishuai Luo0Cuiyi Liao1Cuiyi Liao2Jinju Peng3Songruo Tao4Tengyue Zhang5Yue Dai6Yuexia Ding7Yi Ma8Yi Ma9Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaCollege of Traditional Chinese Medicine, Zhanjiang University of Science and Technology, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaIntroductionThe widespread use of antibiotics in animal agriculture has increased the resistance of Escherichia coli, and pathogenic E. coli often harbor complex virulence factors. Antimicrobial resistance in pathogenic bacteria can cause public health problems. Correlation analyses of the resistance, virulence, and serotype data from the pathogenic bacteria found on farms and in the surrounding environment can thus provide extremely valuable data to help improve public health management.MethodsIn this investigation, we have assessed the drug resistance and virulence genes as well as the molecular typing characteristics of 30 E. coli strains isolated from duck farms in the Zhanjiang area of China. Polymerase chain reaction was used to detect the drug resistance and virulence genes as well as serotypes, and whole-genome sequencing was used to analyze the multilocus sequence typing.ResultsThe detection rates for the oqxA resistance gene and fimC virulence gene were highest (93.3%, respectively). There were no correlations between the drug resistance and virulence gene numbers in the same strain. The epidemic serotype was O81 (5/24), ST3856 was an epidemic sequence type, and strains I-9 and III-6 carried 11 virulence genes. The E. coli strains from the duck farms in the Zhanjiang area were thus found to have a broad drug resistance spectrum, various virulence genes, complex serotypes, and certain pathogenicity and genetic relationship.DiscussionMonitoring the spread of pathogenic bacteria and the provision of guidance regarding the use of antibiotics in the livestock and poultry industries will be required in the future in the Zhanjiang area.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1202013/fullEscherichia colidrug resistance genevirulence genemolecular typingduck farm |
| spellingShingle | Shuaishuai Luo Cuiyi Liao Cuiyi Liao Jinju Peng Songruo Tao Tengyue Zhang Yue Dai Yuexia Ding Yi Ma Yi Ma Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, China Frontiers in Cellular and Infection Microbiology Escherichia coli drug resistance gene virulence gene molecular typing duck farm |
| title | Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, China |
| title_full | Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, China |
| title_fullStr | Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, China |
| title_full_unstemmed | Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, China |
| title_short | Resistance and virulence gene analysis and molecular typing of Escherichia coli from duck farms in Zhanjiang, China |
| title_sort | resistance and virulence gene analysis and molecular typing of escherichia coli from duck farms in zhanjiang china |
| topic | Escherichia coli drug resistance gene virulence gene molecular typing duck farm |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1202013/full |
| work_keys_str_mv | AT shuaishuailuo resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT cuiyiliao resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT cuiyiliao resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT jinjupeng resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT songruotao resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT tengyuezhang resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT yuedai resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT yuexiading resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT yima resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina AT yima resistanceandvirulencegeneanalysisandmoleculartypingofescherichiacolifromduckfarmsinzhanjiangchina |