Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment
Summary: This protocol enables correlative light and electron microscopy (CLEM) imaging of cell surface features without using dedicated equipment. Cells are cultured and fixed on transparent substrates for confocal microscopy imaging. No conductive coating is employed in the scanning electron micro...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004342 |
| _version_ | 1818729171962036224 |
|---|---|
| author | Javier Casares-Arias Miguel A. Alonso Álvaro San Paulo María Ujué González |
| author_facet | Javier Casares-Arias Miguel A. Alonso Álvaro San Paulo María Ujué González |
| author_sort | Javier Casares-Arias |
| collection | DOAJ |
| description | Summary: This protocol enables correlative light and electron microscopy (CLEM) imaging of cell surface features without using dedicated equipment. Cells are cultured and fixed on transparent substrates for confocal microscopy imaging. No conductive coating is employed in the scanning electron microscopy workflow, providing a clean cell surface observation, with fiducial markers assisting alignment of optical and topographical images. This protocol describes CLEM imaging for midbody remnants in MDCK cells but can also be applied to different cell types and surface features.For complete details on the use and execution of this protocol, please refer to Casares-Arias et al. (2020). |
| first_indexed | 2024-12-17T22:41:39Z |
| format | Article |
| id | doaj.art-4cadff1f462a41d0aaec98a8e72580eb |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T22:41:39Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-4cadff1f462a41d0aaec98a8e72580eb2022-12-21T21:29:54ZengElsevierSTAR Protocols2666-16672021-09-0123100727Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipmentJavier Casares-Arias0Miguel A. Alonso1Álvaro San Paulo2María Ujué González3Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain; Corresponding authorCentro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, SpainInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), Tres Cantos, Madrid 28760, SpainInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), Tres Cantos, Madrid 28760, Spain; Corresponding authorSummary: This protocol enables correlative light and electron microscopy (CLEM) imaging of cell surface features without using dedicated equipment. Cells are cultured and fixed on transparent substrates for confocal microscopy imaging. No conductive coating is employed in the scanning electron microscopy workflow, providing a clean cell surface observation, with fiducial markers assisting alignment of optical and topographical images. This protocol describes CLEM imaging for midbody remnants in MDCK cells but can also be applied to different cell types and surface features.For complete details on the use and execution of this protocol, please refer to Casares-Arias et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721004342Cell BiologyMicroscopy |
| spellingShingle | Javier Casares-Arias Miguel A. Alonso Álvaro San Paulo María Ujué González Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment STAR Protocols Cell Biology Microscopy |
| title | Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment |
| title_full | Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment |
| title_fullStr | Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment |
| title_full_unstemmed | Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment |
| title_short | Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment |
| title_sort | correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment |
| topic | Cell Biology Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721004342 |
| work_keys_str_mv | AT javiercasaresarias correlativeconfocalandscanningelectronmicroscopyofculturedcellswithoutusingdedicatedequipment AT miguelaalonso correlativeconfocalandscanningelectronmicroscopyofculturedcellswithoutusingdedicatedequipment AT alvarosanpaulo correlativeconfocalandscanningelectronmicroscopyofculturedcellswithoutusingdedicatedequipment AT mariaujuegonzalez correlativeconfocalandscanningelectronmicroscopyofculturedcellswithoutusingdedicatedequipment |