Correlative confocal and scanning electron microscopy of cultured cells without using dedicated equipment
Summary: This protocol enables correlative light and electron microscopy (CLEM) imaging of cell surface features without using dedicated equipment. Cells are cultured and fixed on transparent substrates for confocal microscopy imaging. No conductive coating is employed in the scanning electron micro...
Main Authors: | Javier Casares-Arias, Miguel A. Alonso, Álvaro San Paulo, María Ujué González |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-09-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004342 |
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