Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation
Whitlockite (WH) is a calcium-phosphate-based Mg-containing ceramic with good mechanical properties, rapid resorption, and good osteogenicity. Recently, we successfully synthesized highly porous WH granules using a marine plankton exoskeleton (MP-WH). In the present study, we improved the osteoinduc...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/8/399 |
| _version_ | 1827618133816377344 |
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| author | Ji Won Baek Ki Su Kim Ho Park Nak Gyu Park Beom-Su Kim |
| author_facet | Ji Won Baek Ki Su Kim Ho Park Nak Gyu Park Beom-Su Kim |
| author_sort | Ji Won Baek |
| collection | DOAJ |
| description | Whitlockite (WH) is a calcium-phosphate-based Mg-containing ceramic with good mechanical properties, rapid resorption, and good osteogenicity. Recently, we successfully synthesized highly porous WH granules using a marine plankton exoskeleton (MP-WH). In the present study, we improved the osteoinductive activity of MP-WH granules by bone morphogenetic protein2 (BMP2) (MP-WH/BMP2). The surface morphology and composition of the fabricated MP-WH/BMP2 granules were characterized using scanning electron microscopy (SEM), X-ray diffraction, and Fourier transform infrared (FT-IR) spectroscopy. The biocompatibility and osteogenic effects were evaluated using human mesenchymal stem cells (hMSCs). BMP2 was absorbed on the surfaces of the MP-WH/BMP2 granules. Immobilized BMP2 was released at a moderate rate over 30 days. hMSCs seeded on MP-WH/BMP2 granules became biocompatible, with a better proliferation and adhesion for MP-WH/BMP2, compared with MP-WH. Bone-specific markers Runx2, type I collagen, osteocalcin, and osteopontin were significantly upregulated following BMP2 incorporation. Similar observations were made regarding the alkaline phosphatase activity. This study suggests that BMP2 incorporation improves the osteoinductive activity of marine-plankton-derived WH granules for bone tissue repair. |
| first_indexed | 2024-03-09T10:01:22Z |
| format | Article |
| id | doaj.art-4cae271ce62947a88358e5a3c0fc53ea |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T10:01:22Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-4cae271ce62947a88358e5a3c0fc53ea2023-12-01T23:25:26ZengMDPI AGBioengineering2306-53542022-08-019839910.3390/bioengineering9080399Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 IncorporationJi Won Baek0Ki Su Kim1Ho Park2Nak Gyu Park3Beom-Su Kim4Department of R&BD, Cellco Inc., 208, Venture Startup Center, Jeonju University, 303, Cheonjam-ro, Wansan-gu, Jeonju-si 55069, KoreaDepartment of R&BD, Cellco Inc., 208, Venture Startup Center, Jeonju University, 303, Cheonjam-ro, Wansan-gu, Jeonju-si 55069, KoreaDepartment of Clinical Laboratory Science, Wonkwang Health Science University, 514, Iksan-daero, Iksan-si 54538, KoreaDivision of Mechanical Design Engineering, Jeonbuk National University, Jeonju-si 54896, KoreaDepartment of R&BD, Cellco Inc., 208, Venture Startup Center, Jeonju University, 303, Cheonjam-ro, Wansan-gu, Jeonju-si 55069, KoreaWhitlockite (WH) is a calcium-phosphate-based Mg-containing ceramic with good mechanical properties, rapid resorption, and good osteogenicity. Recently, we successfully synthesized highly porous WH granules using a marine plankton exoskeleton (MP-WH). In the present study, we improved the osteoinductive activity of MP-WH granules by bone morphogenetic protein2 (BMP2) (MP-WH/BMP2). The surface morphology and composition of the fabricated MP-WH/BMP2 granules were characterized using scanning electron microscopy (SEM), X-ray diffraction, and Fourier transform infrared (FT-IR) spectroscopy. The biocompatibility and osteogenic effects were evaluated using human mesenchymal stem cells (hMSCs). BMP2 was absorbed on the surfaces of the MP-WH/BMP2 granules. Immobilized BMP2 was released at a moderate rate over 30 days. hMSCs seeded on MP-WH/BMP2 granules became biocompatible, with a better proliferation and adhesion for MP-WH/BMP2, compared with MP-WH. Bone-specific markers Runx2, type I collagen, osteocalcin, and osteopontin were significantly upregulated following BMP2 incorporation. Similar observations were made regarding the alkaline phosphatase activity. This study suggests that BMP2 incorporation improves the osteoinductive activity of marine-plankton-derived WH granules for bone tissue repair.https://www.mdpi.com/2306-5354/9/8/399whitlockitemarine planktonbone graftbone morphogenetic protein 2bone regeneration |
| spellingShingle | Ji Won Baek Ki Su Kim Ho Park Nak Gyu Park Beom-Su Kim Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation Bioengineering whitlockite marine plankton bone graft bone morphogenetic protein 2 bone regeneration |
| title | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
| title_full | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
| title_fullStr | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
| title_full_unstemmed | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
| title_short | Enhanced Biocompatibility and Osteogenic Activity of Marine-Plankton-Derived Whitlockite Bone Granules through Bone Morphogenetic Protein 2 Incorporation |
| title_sort | enhanced biocompatibility and osteogenic activity of marine plankton derived whitlockite bone granules through bone morphogenetic protein 2 incorporation |
| topic | whitlockite marine plankton bone graft bone morphogenetic protein 2 bone regeneration |
| url | https://www.mdpi.com/2306-5354/9/8/399 |
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