Immunihistochemical analyses of collagen I and III and elastin in uteri ligaments in female genital prolapse
The frequency of the female genital prolapse varies from 34,1% to 56,3% in different countries. Pathogenesis and morphological changes in uteri ligaments in female genital prolapse are not clear and have to be investigated. The goal of our study was to investigate uteri ligaments in female genital p...
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| Format: | Article |
| Language: | English |
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Peoples’ Friendship University of Russia (RUDN University)
2013-12-01
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| Series: | RUDN Journal of Medicine |
| Subjects: | |
| Online Access: | http://journals.rudn.ru/medicine/article/view/3445 |
| _version_ | 1828314531408904192 |
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| author | M L Khanzadyan R Damien Konnon T A Demura |
| author_facet | M L Khanzadyan R Damien Konnon T A Demura |
| author_sort | M L Khanzadyan |
| collection | DOAJ |
| description | The frequency of the female genital prolapse varies from 34,1% to 56,3% in different countries. Pathogenesis and morphological changes in uteri ligaments in female genital prolapse are not clear and have to be investigated. The goal of our study was to investigate uteri ligaments in female genital prolapse by immunohistochemical analyses of collagen I and III and elastin. The study was performed on biopsies of sacro-uterine and round ligaments from 15 women with genital prolapse and 15 women without prolapse. The tissues were fixed in formalin, embedded in paraffin and cut for the slide. Immunohistochemistry was done with primary antibodies collagen I and III (Novus Biologicus, 1:100 и 1:200 and elastin (Thermo Scientific, 1:100). Morphological analyses of ligaments in prolapse showed fibrosis and elastic fibers fragmentation. Immunohistochemical analyses revealed collagens disbalance: with prevalence of collagen III and decrease in elastin compared with control. Impairment in elastic fibers quantity and distribution affects microcirculation and results in connective tissue desorganisation in uterine ligaments, leading to genital prolapse. Further investigations are needed to reveal diagnostic markers and women at risk. |
| first_indexed | 2024-04-13T16:46:21Z |
| format | Article |
| id | doaj.art-4cb04010b8f244c4ab690115d0c04a5e |
| institution | Directory Open Access Journal |
| issn | 2313-0245 2313-0261 |
| language | English |
| last_indexed | 2024-04-13T16:46:21Z |
| publishDate | 2013-12-01 |
| publisher | Peoples’ Friendship University of Russia (RUDN University) |
| record_format | Article |
| series | RUDN Journal of Medicine |
| spelling | doaj.art-4cb04010b8f244c4ab690115d0c04a5e2022-12-22T02:39:04ZengPeoples’ Friendship University of Russia (RUDN University)RUDN Journal of Medicine2313-02452313-02612013-12-010S51561633445Immunihistochemical analyses of collagen I and III and elastin in uteri ligaments in female genital prolapseM L Khanzadyan0R Damien Konnon1T A Demura2Российский университет дружбы народовРоссийский университет дружбы народовГБОУ ВПО Первый МГМУ им. И.М. СеченоваThe frequency of the female genital prolapse varies from 34,1% to 56,3% in different countries. Pathogenesis and morphological changes in uteri ligaments in female genital prolapse are not clear and have to be investigated. The goal of our study was to investigate uteri ligaments in female genital prolapse by immunohistochemical analyses of collagen I and III and elastin. The study was performed on biopsies of sacro-uterine and round ligaments from 15 women with genital prolapse and 15 women without prolapse. The tissues were fixed in formalin, embedded in paraffin and cut for the slide. Immunohistochemistry was done with primary antibodies collagen I and III (Novus Biologicus, 1:100 и 1:200 and elastin (Thermo Scientific, 1:100). Morphological analyses of ligaments in prolapse showed fibrosis and elastic fibers fragmentation. Immunohistochemical analyses revealed collagens disbalance: with prevalence of collagen III and decrease in elastin compared with control. Impairment in elastic fibers quantity and distribution affects microcirculation and results in connective tissue desorganisation in uterine ligaments, leading to genital prolapse. Further investigations are needed to reveal diagnostic markers and women at risk.http://journals.rudn.ru/medicine/article/view/3445выпадение и опущение внутренних половых органовкрестцово-маточная и круглая связка маткиколлаген I и IIIэластин |
| spellingShingle | M L Khanzadyan R Damien Konnon T A Demura Immunihistochemical analyses of collagen I and III and elastin in uteri ligaments in female genital prolapse RUDN Journal of Medicine выпадение и опущение внутренних половых органов крестцово-маточная и круглая связка матки коллаген I и III эластин |
| title | Immunihistochemical analyses of collagen I and III and elastin in uteri ligaments in female genital prolapse |
| title_full | Immunihistochemical analyses of collagen I and III and elastin in uteri ligaments in female genital prolapse |
| title_fullStr | Immunihistochemical analyses of collagen I and III and elastin in uteri ligaments in female genital prolapse |
| title_full_unstemmed | Immunihistochemical analyses of collagen I and III and elastin in uteri ligaments in female genital prolapse |
| title_short | Immunihistochemical analyses of collagen I and III and elastin in uteri ligaments in female genital prolapse |
| title_sort | immunihistochemical analyses of collagen i and iii and elastin in uteri ligaments in female genital prolapse |
| topic | выпадение и опущение внутренних половых органов крестцово-маточная и круглая связка матки коллаген I и III эластин |
| url | http://journals.rudn.ru/medicine/article/view/3445 |
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