Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats
Background and Aims: Fibromyalgia (FM) is a chronic pain syndrome affected by the hippocampal function. Mesenchymal stem cells-conditioned medium (MSC-CMs) has shown antioxidant and neuroprotective potentials in treating various diseases. The present study aimed to investigate the efficacy of MSC-CM...
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| Format: | Article |
| Language: | fas |
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Birjand University of Medical Sciences and Health Services
2022-12-01
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| Series: | مجله دانشگاه علوم پزشکی بیرجند |
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| Online Access: | http://journal.bums.ac.ir/article-1-3191-en.html |
| _version_ | 1797846414971109376 |
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| author | Farzaneh Jasbinia Majid Hassanpourezatti |
| author_facet | Farzaneh Jasbinia Majid Hassanpourezatti |
| author_sort | Farzaneh Jasbinia |
| collection | DOAJ |
| description | Background and Aims: Fibromyalgia (FM) is a chronic pain syndrome affected by the hippocampal function. Mesenchymal stem cells-conditioned medium (MSC-CMs) has shown antioxidant and neuroprotective potentials in treating various diseases. The present study aimed to investigate the efficacy of MSC-CMs on the thermal treatment of reserpine-induced FM and evaluate the concentration changes of tumor necrosis factor-alpha (TNF-α) in the hippocampus of rats under these conditions.
Materials and Methods: 220 g. Animals received reserpine (1 mg/kg) in the FM groups, and subcutaneous injection of normal saline in other groups, once a day for 3 consecutive days. Then, the rats received MSC-CM (0.3 and 0.5 mg/ml protein content, intraperitoneally) once a week for three weeks. The thermal pain threshold of mice was evaluated using tail flick and hot plate tests at the beginning and end of the experiments. TNF-α concentration in the hippocampus was determined by an ELISA kit at the end of the experimental period.
Results: The thermal pain threshold of rats in the FM group decreased significantly (P<0.01) in both pain tests compared to that of the control group. A significant increase (P<0.01) was observed in the level of TNF-α in the hippocampus of rats in the FM group compared to that of the control group. Treatment with MSC-CMs significantly reduced reserpine-induced pain and hippocampal TNF-a concentration despite the effect of fresh and conditioned medium on control rats.
Conclusion: Treatment with MSC-CMs could control thermal pain in the rat model of reserpine-induced fibromyalgia by controlling the TNF-a level in the hippocampus. |
| first_indexed | 2024-04-09T17:54:32Z |
| format | Article |
| id | doaj.art-4cb128809faf4f17927ae5471a2d26ab |
| institution | Directory Open Access Journal |
| issn | 1607-2197 2423-6152 |
| language | fas |
| last_indexed | 2024-04-09T17:54:32Z |
| publishDate | 2022-12-01 |
| publisher | Birjand University of Medical Sciences and Health Services |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی بیرجند |
| spelling | doaj.art-4cb128809faf4f17927ae5471a2d26ab2023-04-15T05:42:17ZfasBirjand University of Medical Sciences and Health Servicesمجله دانشگاه علوم پزشکی بیرجند1607-21972423-61522022-12-01293218228Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in ratsFarzaneh Jasbinia0Majid Hassanpourezatti1 Master of Science, Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Background and Aims: Fibromyalgia (FM) is a chronic pain syndrome affected by the hippocampal function. Mesenchymal stem cells-conditioned medium (MSC-CMs) has shown antioxidant and neuroprotective potentials in treating various diseases. The present study aimed to investigate the efficacy of MSC-CMs on the thermal treatment of reserpine-induced FM and evaluate the concentration changes of tumor necrosis factor-alpha (TNF-α) in the hippocampus of rats under these conditions. Materials and Methods: 220 g. Animals received reserpine (1 mg/kg) in the FM groups, and subcutaneous injection of normal saline in other groups, once a day for 3 consecutive days. Then, the rats received MSC-CM (0.3 and 0.5 mg/ml protein content, intraperitoneally) once a week for three weeks. The thermal pain threshold of mice was evaluated using tail flick and hot plate tests at the beginning and end of the experiments. TNF-α concentration in the hippocampus was determined by an ELISA kit at the end of the experimental period. Results: The thermal pain threshold of rats in the FM group decreased significantly (P<0.01) in both pain tests compared to that of the control group. A significant increase (P<0.01) was observed in the level of TNF-α in the hippocampus of rats in the FM group compared to that of the control group. Treatment with MSC-CMs significantly reduced reserpine-induced pain and hippocampal TNF-a concentration despite the effect of fresh and conditioned medium on control rats. Conclusion: Treatment with MSC-CMs could control thermal pain in the rat model of reserpine-induced fibromyalgia by controlling the TNF-a level in the hippocampus.http://journal.bums.ac.ir/article-1-3191-en.htmlculture mediumhippocampusinflammationmesenchymal stem cellspainreserpine |
| spellingShingle | Farzaneh Jasbinia Majid Hassanpourezatti Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats مجله دانشگاه علوم پزشکی بیرجند culture medium hippocampus inflammation mesenchymal stem cells pain reserpine |
| title | Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats |
| title_full | Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats |
| title_fullStr | Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats |
| title_full_unstemmed | Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats |
| title_short | Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats |
| title_sort | treatment with conditioned culture media with human mesenchymal stem cells on reserpine induced thermal fibromyalgia model in rats |
| topic | culture medium hippocampus inflammation mesenchymal stem cells pain reserpine |
| url | http://journal.bums.ac.ir/article-1-3191-en.html |
| work_keys_str_mv | AT farzanehjasbinia treatmentwithconditionedculturemediawithhumanmesenchymalstemcellsonreserpineinducedthermalfibromyalgiamodelinrats AT majidhassanpourezatti treatmentwithconditionedculturemediawithhumanmesenchymalstemcellsonreserpineinducedthermalfibromyalgiamodelinrats |