Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii
This study, the presence of 4 common beta-lactamase encoding genes including OXA-23, OXA-51, VIM and IMP was determined simultaneously by multiplex PCR, either directly with sputum samples or with purified DNA, giving results within 3-4 hours instead of 2 days compared to the culture method. Within...
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| Format: | Article |
| Language: | English |
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Vietnam Ministry of Science and Technology
2022-04-01
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| Series: | Vietnam Journal of Science, Technology and Engineering |
| Subjects: | |
| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/200 |
| _version_ | 1797937748454146048 |
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| author | Le Phuong Trinh Van Son Ta Thi Ngoc Han Luu Mai Phuong Nguyen Thi Huong Dinh Van Hung Nguyen Thi Ha Doan Bao Yen Pham |
| author_facet | Le Phuong Trinh Van Son Ta Thi Ngoc Han Luu Mai Phuong Nguyen Thi Huong Dinh Van Hung Nguyen Thi Ha Doan Bao Yen Pham |
| author_sort | Le Phuong Trinh |
| collection | DOAJ |
| description |
This study, the presence of 4 common beta-lactamase encoding genes including OXA-23, OXA-51, VIM and IMP was determined simultaneously by multiplex PCR, either directly with sputum samples or with purified DNA, giving results within 3-4 hours instead of 2 days compared to the culture method. Within 58 samples positive with Acinetobacter baumannii collected from the hospital, OXA-51 fragment was dominant (81%), whereas, VIM fragment was only identified in one sample (DNA and sputum samples) (1.7%). The antibiograms indicated for 19 samples were used to find correlation between genotype and resistance phenotype.
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| first_indexed | 2024-04-10T18:50:15Z |
| format | Article |
| id | doaj.art-4cb458c1ad39431bab920c22b9b6ecbf |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-10T18:50:15Z |
| publishDate | 2022-04-01 |
| publisher | Vietnam Ministry of Science and Technology |
| record_format | Article |
| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-4cb458c1ad39431bab920c22b9b6ecbf2023-02-01T08:12:48ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372022-04-0161110.31276/VJSTE.61(1).58-61Studies of common antibiotic resistance-associated genes of Acinetobacter baumanniiLe Phuong Trinh0Van Son Ta1Thi Ngoc Han Luu2Mai Phuong Nguyen3Thi Huong Dinh4Van Hung Nguyen5Thi Ha Doan6Bao Yen Pham7Key Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi; Faculty of Biology, University of Science, Vietnam National University, HanoiFaculty of Biology, University of Science, Vietnam National University, HanoiFaculty of Biology, University of Science, Vietnam National University, HanoiKey Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi; Faculty of Biology, University of Science, Vietnam National University, HanoiNational Lung HospitalNational Lung HospitalNational Lung HospitalKey Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi; Faculty of Biology, University of Science, Vietnam National University, Hanoi This study, the presence of 4 common beta-lactamase encoding genes including OXA-23, OXA-51, VIM and IMP was determined simultaneously by multiplex PCR, either directly with sputum samples or with purified DNA, giving results within 3-4 hours instead of 2 days compared to the culture method. Within 58 samples positive with Acinetobacter baumannii collected from the hospital, OXA-51 fragment was dominant (81%), whereas, VIM fragment was only identified in one sample (DNA and sputum samples) (1.7%). The antibiograms indicated for 19 samples were used to find correlation between genotype and resistance phenotype. https://vietnamscience.vjst.vn/index.php/vjste/article/view/200Acinetobacter baumanniiantibiotic resistance-associated genesbeta-lactamasemultiplex PCRsusceptibility |
| spellingShingle | Le Phuong Trinh Van Son Ta Thi Ngoc Han Luu Mai Phuong Nguyen Thi Huong Dinh Van Hung Nguyen Thi Ha Doan Bao Yen Pham Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii Vietnam Journal of Science, Technology and Engineering Acinetobacter baumannii antibiotic resistance-associated genes beta-lactamase multiplex PCR susceptibility |
| title | Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii |
| title_full | Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii |
| title_fullStr | Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii |
| title_full_unstemmed | Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii |
| title_short | Studies of common antibiotic resistance-associated genes of Acinetobacter baumannii |
| title_sort | studies of common antibiotic resistance associated genes of acinetobacter baumannii |
| topic | Acinetobacter baumannii antibiotic resistance-associated genes beta-lactamase multiplex PCR susceptibility |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/200 |
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