An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips
<p>Abstract</p> <p>Background</p> <p>Tissue microarray technology has provided a high throughput means of evaluating potential biomarkers in archival pathological specimens. This study was carried out in order to produce tissue microarray blocks using mechanical pencil...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-12-01
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| Series: | Diagnostic Pathology |
| Subjects: | |
| Online Access: | http://www.diagnosticpathology.org/content/6/1/117 |
| _version_ | 1819036652895469568 |
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| author | Shebl Abdelhadi M Zalata Khaled R Amin Maha M El-Hawary Amira K |
| author_facet | Shebl Abdelhadi M Zalata Khaled R Amin Maha M El-Hawary Amira K |
| author_sort | Shebl Abdelhadi M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Tissue microarray technology has provided a high throughput means of evaluating potential biomarkers in archival pathological specimens. This study was carried out in order to produce tissue microarray blocks using mechanical pencil tips without high cost.</p> <p>Method</p> <p>Conventional mechanical pencil tips (Rotring Tikky II Mechanical Pencil 1.0 mm) were used to cut out 1 mm wax cylinders from the recipient block, creating from 36 to 72 holes. Three cores of tumor areas were punched out manually by using the mechanical pencil tips from donor paraffin embedded tissue blocks and transferred to the holes of the paraffin tissue microarrays.</p> <p>Results</p> <p>This technique was easy and caused little damage to the donor blocks. We successfully performed H&E slides and immunodetection without substantial tissue cylinder loss.</p> <p>Conclusion</p> <p>Our mechanical pencil tip technique is the most inexpensive easy technique among the literature. It also takes a reasonable amount of time and reduces antibody consumption during immunohistochemistry</p> |
| first_indexed | 2024-12-21T08:08:56Z |
| format | Article |
| id | doaj.art-4cb48e84552b4d1fa05970fcbfb53cc5 |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-12-21T08:08:56Z |
| publishDate | 2011-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-4cb48e84552b4d1fa05970fcbfb53cc52022-12-21T19:10:42ZengBMCDiagnostic Pathology1746-15962011-12-016111710.1186/1746-1596-6-117An inexpensive method of small paraffin tissue microarrays using mechanical pencil tipsShebl Abdelhadi MZalata Khaled RAmin Maha MEl-Hawary Amira K<p>Abstract</p> <p>Background</p> <p>Tissue microarray technology has provided a high throughput means of evaluating potential biomarkers in archival pathological specimens. This study was carried out in order to produce tissue microarray blocks using mechanical pencil tips without high cost.</p> <p>Method</p> <p>Conventional mechanical pencil tips (Rotring Tikky II Mechanical Pencil 1.0 mm) were used to cut out 1 mm wax cylinders from the recipient block, creating from 36 to 72 holes. Three cores of tumor areas were punched out manually by using the mechanical pencil tips from donor paraffin embedded tissue blocks and transferred to the holes of the paraffin tissue microarrays.</p> <p>Results</p> <p>This technique was easy and caused little damage to the donor blocks. We successfully performed H&E slides and immunodetection without substantial tissue cylinder loss.</p> <p>Conclusion</p> <p>Our mechanical pencil tip technique is the most inexpensive easy technique among the literature. It also takes a reasonable amount of time and reduces antibody consumption during immunohistochemistry</p>http://www.diagnosticpathology.org/content/6/1/117Paraffin tissuemicroarrays-mechanicalpencil tips |
| spellingShingle | Shebl Abdelhadi M Zalata Khaled R Amin Maha M El-Hawary Amira K An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips Diagnostic Pathology Paraffin tissue microarrays-mechanical pencil tips |
| title | An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips |
| title_full | An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips |
| title_fullStr | An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips |
| title_full_unstemmed | An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips |
| title_short | An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips |
| title_sort | inexpensive method of small paraffin tissue microarrays using mechanical pencil tips |
| topic | Paraffin tissue microarrays-mechanical pencil tips |
| url | http://www.diagnosticpathology.org/content/6/1/117 |
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