A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug Targets
Detailed knowledge of cell-surface proteins for isolating well-defined populations of human pluripotent stem cells (hPSCs) would significantly enhance their characterization and translational potential. Through a chemoproteomic approach, we developed a cell-surface proteome inventory containing 496...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-07-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221367111400143X |
| _version_ | 1828236468236058624 |
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| author | Kenneth R. Boheler Subarna Bhattacharya Erin M. Kropp Sandra Chuppa Daniel R. Riordon Damaris Bausch-Fluck Paul W. Burridge Joseph C. Wu Robert P. Wersto Godfrey Chi Fung Chan Sridhar Rao Bernd Wollscheid Rebekah L. Gundry |
| author_facet | Kenneth R. Boheler Subarna Bhattacharya Erin M. Kropp Sandra Chuppa Daniel R. Riordon Damaris Bausch-Fluck Paul W. Burridge Joseph C. Wu Robert P. Wersto Godfrey Chi Fung Chan Sridhar Rao Bernd Wollscheid Rebekah L. Gundry |
| author_sort | Kenneth R. Boheler |
| collection | DOAJ |
| description | Detailed knowledge of cell-surface proteins for isolating well-defined populations of human pluripotent stem cells (hPSCs) would significantly enhance their characterization and translational potential. Through a chemoproteomic approach, we developed a cell-surface proteome inventory containing 496 N-linked glycoproteins on human embryonic (hESCs) and induced PSCs (hiPSCs). Against a backdrop of human fibroblasts and 50 other cell types, >100 surface proteins of interest for hPSCs were revealed. The >30 positive and negative markers verified here by orthogonal approaches provide experimental justification for the rational selection of pluripotency and lineage markers, epitopes for cell isolation, and reagents for the characterization of putative hiPSC lines. Comparative differences between the chemoproteomic-defined surfaceome and the transcriptome-predicted surfaceome directly led to the discovery that STF-31, a reported GLUT-1 inhibitor, is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures. |
| first_indexed | 2024-04-12T20:33:38Z |
| format | Article |
| id | doaj.art-4cb4d4fca3d44ea7ab17c6b73157557c |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-12T20:33:38Z |
| publishDate | 2014-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-4cb4d4fca3d44ea7ab17c6b73157557c2022-12-22T03:17:40ZengElsevierStem Cell Reports2213-67112014-07-013118520310.1016/j.stemcr.2014.05.002A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug TargetsKenneth R. Boheler0Subarna Bhattacharya1Erin M. Kropp2Sandra Chuppa3Daniel R. Riordon4Damaris Bausch-Fluck5Paul W. Burridge6Joseph C. Wu7Robert P. Wersto8Godfrey Chi Fung Chan9Sridhar Rao10Bernd Wollscheid11Rebekah L. Gundry12Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, Hong Kong, SARDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USADepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USADepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USANational Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USADepartment of Biology, Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 16, 8093 Zurich, SwitzerlandStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USANational Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USAStem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, Hong Kong, SARDepartment of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USADepartment of Biology, Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 16, 8093 Zurich, SwitzerlandDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USADetailed knowledge of cell-surface proteins for isolating well-defined populations of human pluripotent stem cells (hPSCs) would significantly enhance their characterization and translational potential. Through a chemoproteomic approach, we developed a cell-surface proteome inventory containing 496 N-linked glycoproteins on human embryonic (hESCs) and induced PSCs (hiPSCs). Against a backdrop of human fibroblasts and 50 other cell types, >100 surface proteins of interest for hPSCs were revealed. The >30 positive and negative markers verified here by orthogonal approaches provide experimental justification for the rational selection of pluripotency and lineage markers, epitopes for cell isolation, and reagents for the characterization of putative hiPSC lines. Comparative differences between the chemoproteomic-defined surfaceome and the transcriptome-predicted surfaceome directly led to the discovery that STF-31, a reported GLUT-1 inhibitor, is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures.http://www.sciencedirect.com/science/article/pii/S221367111400143X |
| spellingShingle | Kenneth R. Boheler Subarna Bhattacharya Erin M. Kropp Sandra Chuppa Daniel R. Riordon Damaris Bausch-Fluck Paul W. Burridge Joseph C. Wu Robert P. Wersto Godfrey Chi Fung Chan Sridhar Rao Bernd Wollscheid Rebekah L. Gundry A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug Targets Stem Cell Reports |
| title | A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug Targets |
| title_full | A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug Targets |
| title_fullStr | A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug Targets |
| title_full_unstemmed | A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug Targets |
| title_short | A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug Targets |
| title_sort | human pluripotent stem cell surface n glycoproteome resource reveals markers extracellular epitopes and drug targets |
| url | http://www.sciencedirect.com/science/article/pii/S221367111400143X |
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