ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS
Tick borne encephalitis (TBE) is a dangerous neurological disease that is transmitted to humans through the bite of Ixodid ticks. The disease exhibits an estimated 16 000 cases recorded annually over 30 European and Asian countries. The agent of TBE is the tick borne encephalitis virus (TBEV), belon...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2017-02-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/369 |
| _version_ | 1797237401517555712 |
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| author | I. S. Solovarov M. A. Khasnatinov G. A. Danchinova A. V. Liapunov N. A. Bolotova E. L. Manzarova I. G. Kondratov S. I. Belikov |
| author_facet | I. S. Solovarov M. A. Khasnatinov G. A. Danchinova A. V. Liapunov N. A. Bolotova E. L. Manzarova I. G. Kondratov S. I. Belikov |
| author_sort | I. S. Solovarov |
| collection | DOAJ |
| description | Tick borne encephalitis (TBE) is a dangerous neurological disease that is transmitted to humans through the bite of Ixodid ticks. The disease exhibits an estimated 16 000 cases recorded annually over 30 European and Asian countries. The agent of TBE is the tick borne encephalitis virus (TBEV), belonging to the family Flaviviridae, genus Flavivirus. In spite the significant impact of TBE on human health, there is a serious lack of specific treatment against this disease. The only specific drug available is the human anti-TBEV immunoglobulin from vaccinated blood donors. The drug is produced and used in Russia only, both to prevent and to cure the TBE. In this work, we evaluated the ability of TBEV-specific DNA-aptamers and extracts of traditional medicine plants to neutralize the TBEV Selection of aptamers was performed using SELEX approach. Extracts of the seeds of Momordica cochinchinensis and Terminalia chebula were produced by boiling the ground seeds in water, clarified by centrifugation and filtration steps and filter sterilized. The SELEX had produced two aptamers - My13 and My38. Neither of two was capable to neutralize TBEV in vitro. The ability to shield the antibody binding sites on the surface of TBEV virions was also absent. The extract of M. cochinchinensis exhibited no neutralizing activity as well. Surprisingly, the T. chebula extract completely neutralized the TBEV after 30 min of incubation at 37 °C. The possible explanations and further development of the project are discussed. |
| first_indexed | 2024-03-08T12:18:52Z |
| format | Article |
| id | doaj.art-4cb60a9be9694de18acc44eb946be947 |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:19:10Z |
| publishDate | 2017-02-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-4cb60a9be9694de18acc44eb946be9472024-03-28T07:49:17ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962017-02-0121848810.12737/article_5955e6b5aad2e3.30269730369ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUSI. S. Solovarov0M. A. Khasnatinov1G. A. Danchinova2A. V. Liapunov3N. A. Bolotova4E. L. Manzarova5I. G. Kondratov6S. I. Belikov7Scientific Centre for Family Health and Human Reproduction ProblemsScientific Centre for Family Health and Human Reproduction ProblemsScientific Centre for Family Health and Human Reproduction ProblemsScientific Centre for Family Health and Human Reproduction ProblemsScientific Centre for Family Health and Human Reproduction ProblemsScientific Centre for Family Health and Human Reproduction ProblemsLimnological Institute of Siberian Branch of the Russian Academy of SciencesLimnological Institute of Siberian Branch of the Russian Academy of SciencesTick borne encephalitis (TBE) is a dangerous neurological disease that is transmitted to humans through the bite of Ixodid ticks. The disease exhibits an estimated 16 000 cases recorded annually over 30 European and Asian countries. The agent of TBE is the tick borne encephalitis virus (TBEV), belonging to the family Flaviviridae, genus Flavivirus. In spite the significant impact of TBE on human health, there is a serious lack of specific treatment against this disease. The only specific drug available is the human anti-TBEV immunoglobulin from vaccinated blood donors. The drug is produced and used in Russia only, both to prevent and to cure the TBE. In this work, we evaluated the ability of TBEV-specific DNA-aptamers and extracts of traditional medicine plants to neutralize the TBEV Selection of aptamers was performed using SELEX approach. Extracts of the seeds of Momordica cochinchinensis and Terminalia chebula were produced by boiling the ground seeds in water, clarified by centrifugation and filtration steps and filter sterilized. The SELEX had produced two aptamers - My13 and My38. Neither of two was capable to neutralize TBEV in vitro. The ability to shield the antibody binding sites on the surface of TBEV virions was also absent. The extract of M. cochinchinensis exhibited no neutralizing activity as well. Surprisingly, the T. chebula extract completely neutralized the TBEV after 30 min of incubation at 37 °C. The possible explanations and further development of the project are discussed.https://www.actabiomedica.ru/jour/article/view/369aptamerstick-borne encephalitis virusplaque-forming units |
| spellingShingle | I. S. Solovarov M. A. Khasnatinov G. A. Danchinova A. V. Liapunov N. A. Bolotova E. L. Manzarova I. G. Kondratov S. I. Belikov ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS Acta Biomedica Scientifica aptamers tick-borne encephalitis virus plaque-forming units |
| title | ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS |
| title_full | ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS |
| title_fullStr | ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS |
| title_full_unstemmed | ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS |
| title_short | ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS |
| title_sort | assessment of neutralizing properties of dna aptamers and extracts of medicinal herbs against the tick borne encephalitis virus |
| topic | aptamers tick-borne encephalitis virus plaque-forming units |
| url | https://www.actabiomedica.ru/jour/article/view/369 |
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