Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress
Abstract Background Salinization seriously threatens land use efficiency and crop yields across the world. Understanding the mechanisms plants use to protect against salt stress will help breeders develop salt-tolerant vegetable crops. Okra (Abelmoschus esculentus L.) is an important vegetable crop...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-05-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-019-5737-7 |
| _version_ | 1811324427384651776 |
|---|---|
| author | Yihua Zhan Qingfei Wu Yue Chen Mengling Tang Chendong Sun Junwei Sun Chenliang Yu |
| author_facet | Yihua Zhan Qingfei Wu Yue Chen Mengling Tang Chendong Sun Junwei Sun Chenliang Yu |
| author_sort | Yihua Zhan |
| collection | DOAJ |
| description | Abstract Background Salinization seriously threatens land use efficiency and crop yields across the world. Understanding the mechanisms plants use to protect against salt stress will help breeders develop salt-tolerant vegetable crops. Okra (Abelmoschus esculentus L.) is an important vegetable crop of the mallow family, which is now cultivated in warm regions worldwide. To understand the effects of salt stress on the protein level of okra, a comparative proteomic analysis of okra seedlings grown in the presence of 0 or 300 mmol L− 1 NaCl treatment was performed using an integrated approach of Tandem Mass Tag labeling and LC-MS/MS integrated approach. Results A total of 7179 proteins were identified in this study, for which quantitative information was available for 5774 proteins. In the NaCl/control comparison group, there were 317 differentially expressed proteins (DEPs), of which 165 proteins were upregulated and 152 proteins downregulated in the presence of NaCl. Based on the above data, we carried out a systematic bioinformatics analysis of proteins with information, including protein annotation, domain characteristics, functional classification, and pathway enrichment. Enriched gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the DEPs were most strongly associated with “response to stress” and “protein processing in endoplasmic reticulum”. Furthermore, several heat shock proteins were identified as DEPs. Conclusions This information provides a reference direction for further research on the okra proteome in the downstream of the salt stress response, with our data revealing that the responses of okra to salt stress involves by various pathways. |
| first_indexed | 2024-04-13T14:14:04Z |
| format | Article |
| id | doaj.art-4cb83c73774040d9a0689ad6cd3f0465 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T14:14:04Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-4cb83c73774040d9a0689ad6cd3f04652022-12-22T02:43:41ZengBMCBMC Genomics1471-21642019-05-0120111210.1186/s12864-019-5737-7Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stressYihua Zhan0Qingfei Wu1Yue Chen2Mengling Tang3Chendong Sun4Junwei Sun5Chenliang Yu6State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityInstitute of Horticulture, Zhejiang Academy of Agriculture ScienceThe Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A&F UniversityThe Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A&F UniversityCollege of Modern Science and Technology, China Jiliang UniversityInstitute of Agricultural Equipment, Zhejiang Academy of Agricultural SciencesAbstract Background Salinization seriously threatens land use efficiency and crop yields across the world. Understanding the mechanisms plants use to protect against salt stress will help breeders develop salt-tolerant vegetable crops. Okra (Abelmoschus esculentus L.) is an important vegetable crop of the mallow family, which is now cultivated in warm regions worldwide. To understand the effects of salt stress on the protein level of okra, a comparative proteomic analysis of okra seedlings grown in the presence of 0 or 300 mmol L− 1 NaCl treatment was performed using an integrated approach of Tandem Mass Tag labeling and LC-MS/MS integrated approach. Results A total of 7179 proteins were identified in this study, for which quantitative information was available for 5774 proteins. In the NaCl/control comparison group, there were 317 differentially expressed proteins (DEPs), of which 165 proteins were upregulated and 152 proteins downregulated in the presence of NaCl. Based on the above data, we carried out a systematic bioinformatics analysis of proteins with information, including protein annotation, domain characteristics, functional classification, and pathway enrichment. Enriched gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the DEPs were most strongly associated with “response to stress” and “protein processing in endoplasmic reticulum”. Furthermore, several heat shock proteins were identified as DEPs. Conclusions This information provides a reference direction for further research on the okra proteome in the downstream of the salt stress response, with our data revealing that the responses of okra to salt stress involves by various pathways.http://link.springer.com/article/10.1186/s12864-019-5737-7Differential expression proteinHeat shock proteinsOkraProteomicsSalt stressTMT labeling |
| spellingShingle | Yihua Zhan Qingfei Wu Yue Chen Mengling Tang Chendong Sun Junwei Sun Chenliang Yu Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress BMC Genomics Differential expression protein Heat shock proteins Okra Proteomics Salt stress TMT labeling |
| title | Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress |
| title_full | Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress |
| title_fullStr | Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress |
| title_full_unstemmed | Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress |
| title_short | Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress |
| title_sort | comparative proteomic analysis of okra abelmoschus esculentus l seedlings under salt stress |
| topic | Differential expression protein Heat shock proteins Okra Proteomics Salt stress TMT labeling |
| url | http://link.springer.com/article/10.1186/s12864-019-5737-7 |
| work_keys_str_mv | AT yihuazhan comparativeproteomicanalysisofokraabelmoschusesculentuslseedlingsundersaltstress AT qingfeiwu comparativeproteomicanalysisofokraabelmoschusesculentuslseedlingsundersaltstress AT yuechen comparativeproteomicanalysisofokraabelmoschusesculentuslseedlingsundersaltstress AT menglingtang comparativeproteomicanalysisofokraabelmoschusesculentuslseedlingsundersaltstress AT chendongsun comparativeproteomicanalysisofokraabelmoschusesculentuslseedlingsundersaltstress AT junweisun comparativeproteomicanalysisofokraabelmoschusesculentuslseedlingsundersaltstress AT chenliangyu comparativeproteomicanalysisofokraabelmoschusesculentuslseedlingsundersaltstress |