Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker
BackgroundCentromere protein L (CENPL) is an important member of the centromere protein (CENP) family. However, the correlation between CENPL expression and cancer development and immune infiltration has rarely been studied. Here, we studied the role of CENPL in pan-cancer and further verified the r...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.916594/full |
| _version_ | 1811329198173716480 |
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| author | Ziyang Feng Ziyang Feng Yu Chen Changjing Cai Changjing Cai Jun Tan Ping Liu Ping Liu Yihong Chen Yihong Chen Hong Shen Hong Shen Hong Shen Shan Zeng Shan Zeng Ying Han Ying Han |
| author_facet | Ziyang Feng Ziyang Feng Yu Chen Changjing Cai Changjing Cai Jun Tan Ping Liu Ping Liu Yihong Chen Yihong Chen Hong Shen Hong Shen Hong Shen Shan Zeng Shan Zeng Ying Han Ying Han |
| author_sort | Ziyang Feng |
| collection | DOAJ |
| description | BackgroundCentromere protein L (CENPL) is an important member of the centromere protein (CENP) family. However, the correlation between CENPL expression and cancer development and immune infiltration has rarely been studied. Here, we studied the role of CENPL in pan-cancer and further verified the results in lung adenocarcinoma (LUAD) through in vitro experiments.MethodsThe CENPL expression level was studied with TIMER 2.0 and Oncomine databases. The potential value of CENPL as a diagnostic and prognostic biomarker in pan-cancer was evaluated with the TCGA database and GEPIA. The CENPL mutation character was analyzed using the cBioPortal database. The LinkedOmics and CancerSEA databases were used to carry out the function analysis of CENPL. The role of CENPL in immune infiltration was studied using the TIMER and TISIDB websites. Moreover, the expression of CENPL was detected through RT-qPCR and Western blotting. Immunohistochemistry was used to evaluate the infiltration level of CD8+ T cells. Cell proliferation was detected by EdU and CCK8. A flow cytometer was used to analyze the influence of CENPL in cell cycle and apoptosis.ResultsCENPL was increased in most of the cancers. The upregulation and mutation of CENPL were associated with a poorer prognosis in many cancers. The results showed a significant positive correlation between CENPL and myeloid-derived suppressor cell (MDSC) infiltration and a negative correlation between CENPL and T-cell NK infiltration in most of the cancers. CENPL regulated cell proliferation and cell cycle, and was negatively correlated with the inflammation level of LUAD. The in vitro experiments suggested that CENPL was increased in LUAD tissue and cell lines. There was a negative correlation between CENPL expression and CD8+ T-cell infiltration. The knockdown of CENPL significantly suppressed the expression of CDK2 and CCNE2, and induced G0/G1 arrest and apoptosis of LUAD.ConclusionsCENPL may function as a potential biomarker and oncogene in pan-cancer, especially LUAD. Furthermore, CENPL was associated with immune cell infiltration in pan-cancer, providing a potential immune therapy target for tumor treatment. |
| first_indexed | 2024-04-13T15:39:31Z |
| format | Article |
| id | doaj.art-4cb8b76d71034ebfa64739d648f5062e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T15:39:31Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-4cb8b76d71034ebfa64739d648f5062e2022-12-22T02:41:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-06-011310.3389/fimmu.2022.916594916594Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related BiomarkerZiyang Feng0Ziyang Feng1Yu Chen2Changjing Cai3Changjing Cai4Jun Tan5Ping Liu6Ping Liu7Yihong Chen8Yihong Chen9Hong Shen10Hong Shen11Hong Shen12Shan Zeng13Shan Zeng14Ying Han15Ying Han16Department of Oncology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory for Molecular Radiation Oncology of Hunan Province, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory for Molecular Radiation Oncology of Hunan Province, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory for Molecular Radiation Oncology of Hunan Province, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory for Molecular Radiation Oncology of Hunan Province, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory for Molecular Radiation Oncology of Hunan Province, Xiangya Hospital, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory for Molecular Radiation Oncology of Hunan Province, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory for Molecular Radiation Oncology of Hunan Province, Xiangya Hospital, Central South University, Changsha, ChinaBackgroundCentromere protein L (CENPL) is an important member of the centromere protein (CENP) family. However, the correlation between CENPL expression and cancer development and immune infiltration has rarely been studied. Here, we studied the role of CENPL in pan-cancer and further verified the results in lung adenocarcinoma (LUAD) through in vitro experiments.MethodsThe CENPL expression level was studied with TIMER 2.0 and Oncomine databases. The potential value of CENPL as a diagnostic and prognostic biomarker in pan-cancer was evaluated with the TCGA database and GEPIA. The CENPL mutation character was analyzed using the cBioPortal database. The LinkedOmics and CancerSEA databases were used to carry out the function analysis of CENPL. The role of CENPL in immune infiltration was studied using the TIMER and TISIDB websites. Moreover, the expression of CENPL was detected through RT-qPCR and Western blotting. Immunohistochemistry was used to evaluate the infiltration level of CD8+ T cells. Cell proliferation was detected by EdU and CCK8. A flow cytometer was used to analyze the influence of CENPL in cell cycle and apoptosis.ResultsCENPL was increased in most of the cancers. The upregulation and mutation of CENPL were associated with a poorer prognosis in many cancers. The results showed a significant positive correlation between CENPL and myeloid-derived suppressor cell (MDSC) infiltration and a negative correlation between CENPL and T-cell NK infiltration in most of the cancers. CENPL regulated cell proliferation and cell cycle, and was negatively correlated with the inflammation level of LUAD. The in vitro experiments suggested that CENPL was increased in LUAD tissue and cell lines. There was a negative correlation between CENPL expression and CD8+ T-cell infiltration. The knockdown of CENPL significantly suppressed the expression of CDK2 and CCNE2, and induced G0/G1 arrest and apoptosis of LUAD.ConclusionsCENPL may function as a potential biomarker and oncogene in pan-cancer, especially LUAD. Furthermore, CENPL was associated with immune cell infiltration in pan-cancer, providing a potential immune therapy target for tumor treatment.https://www.frontiersin.org/articles/10.3389/fimmu.2022.916594/fullpan-cancerCENPLimmune infiltrationLUADG0/G1 arrestapoptosis |
| spellingShingle | Ziyang Feng Ziyang Feng Yu Chen Changjing Cai Changjing Cai Jun Tan Ping Liu Ping Liu Yihong Chen Yihong Chen Hong Shen Hong Shen Hong Shen Shan Zeng Shan Zeng Ying Han Ying Han Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker Frontiers in Immunology pan-cancer CENPL immune infiltration LUAD G0/G1 arrest apoptosis |
| title | Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker |
| title_full | Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker |
| title_fullStr | Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker |
| title_full_unstemmed | Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker |
| title_short | Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker |
| title_sort | pan cancer and single cell analysis reveals cenpl as a cancer prognosis and immune infiltration related biomarker |
| topic | pan-cancer CENPL immune infiltration LUAD G0/G1 arrest apoptosis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.916594/full |
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