Ameliorative Effect of Imperatorin on <i>Dermatophagoides pteronyssinus</i>-Induced Allergic Asthma by Suppressing the Th2 Response in Mice
Imperatorin is a furanocoumarin derivative and an effective ingredient in several Chinese medicinal herbs. It has favorable expectorant, analgesic, and anti-inflammatory effects. In this study, we investigated whether imperatorin has protective effects against <i>Dermatophagoides pteronyssinus...
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MDPI AG
2022-10-01
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| author | Chia-Chen Hsieh Yan-Yan Ng Wei-Sung Li Chung-Yuh Tzeng Tsai-Yi Hsu Wan-Hsiang Huang Jen-Chieh Tsai |
| author_facet | Chia-Chen Hsieh Yan-Yan Ng Wei-Sung Li Chung-Yuh Tzeng Tsai-Yi Hsu Wan-Hsiang Huang Jen-Chieh Tsai |
| author_sort | Chia-Chen Hsieh |
| collection | DOAJ |
| description | Imperatorin is a furanocoumarin derivative and an effective ingredient in several Chinese medicinal herbs. It has favorable expectorant, analgesic, and anti-inflammatory effects. In this study, we investigated whether imperatorin has protective effects against <i>Dermatophagoides pteronyssinus</i> (<i>Der p</i>)-induced asthma in mice. Lung and bronchial tissues were histopathologically examined through hematoxylin–eosin staining. The concentrations of immunoglobin E (IgE), IgG1, IgG2a in serum and those of T helper 1 (Th1) and two cytokines and eosinophil-activated chemokines in bronchoalveolar lavage fluid (BALF) were detected using an enzyme immunoassay. Histological examination revealed that imperatorin reduced inflammatory cell infiltration, mucus hypersecretion, and endothelial cell hyperplasia. The examination also indicated that imperatorin could reduce the inflammatory cell count in BALF as well as IgE and IgG1 expression in serum, but IgG2a expression was significantly increased. Imperatorin reduced the production of interleukin (IL)-4, IL-5, and IL-13 by Th2, promoted the production of interferon-γ and IL-12 by Th1, and increased the production of IL-10 in bronchoalveolar lavage fluid. These findings suggest that imperatorin has a considerable anti-inflammatory effect on <i>Der p</i>-induced allergic asthma in mice. |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T19:41:16Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4cba694dfc2b4c40abc869585366db1e2023-11-24T01:35:57ZengMDPI AGMolecules1420-30492022-10-012720702810.3390/molecules27207028Ameliorative Effect of Imperatorin on <i>Dermatophagoides pteronyssinus</i>-Induced Allergic Asthma by Suppressing the Th2 Response in MiceChia-Chen Hsieh0Yan-Yan Ng1Wei-Sung Li2Chung-Yuh Tzeng3Tsai-Yi Hsu4Wan-Hsiang Huang5Jen-Chieh Tsai6Department of Medicine Division of Chest Medicine, Cheng Ching Hospital, Taichung 40764, TaiwanDepartment of Pediatrics, Chung-Kang Branch, Cheng Ching Hospital, Taichung 40764, TaiwanPlant Pathology Division, Taiwan Agricultural Research Institute, Council of Agriculture, Executive Yuan, Wufeng 41362, TaiwanDepartment of Medicinal Botanicals and Foods on Health Applications, Da-Yeh University, ChangHua 51591, TaiwanDepartment of Bioresources, Da-Yeh University, Changhua 51591, TaiwanDepartment of Medicinal Botanicals and Foods on Health Applications, Da-Yeh University, ChangHua 51591, TaiwanDepartment of Medicinal Botanicals and Foods on Health Applications, Da-Yeh University, ChangHua 51591, TaiwanImperatorin is a furanocoumarin derivative and an effective ingredient in several Chinese medicinal herbs. It has favorable expectorant, analgesic, and anti-inflammatory effects. In this study, we investigated whether imperatorin has protective effects against <i>Dermatophagoides pteronyssinus</i> (<i>Der p</i>)-induced asthma in mice. Lung and bronchial tissues were histopathologically examined through hematoxylin–eosin staining. The concentrations of immunoglobin E (IgE), IgG1, IgG2a in serum and those of T helper 1 (Th1) and two cytokines and eosinophil-activated chemokines in bronchoalveolar lavage fluid (BALF) were detected using an enzyme immunoassay. Histological examination revealed that imperatorin reduced inflammatory cell infiltration, mucus hypersecretion, and endothelial cell hyperplasia. The examination also indicated that imperatorin could reduce the inflammatory cell count in BALF as well as IgE and IgG1 expression in serum, but IgG2a expression was significantly increased. Imperatorin reduced the production of interleukin (IL)-4, IL-5, and IL-13 by Th2, promoted the production of interferon-γ and IL-12 by Th1, and increased the production of IL-10 in bronchoalveolar lavage fluid. These findings suggest that imperatorin has a considerable anti-inflammatory effect on <i>Der p</i>-induced allergic asthma in mice.https://www.mdpi.com/1420-3049/27/20/7028imperatorinasthma<i>Dermatophagoides pteronyssinus</i>Th2 response |
| spellingShingle | Chia-Chen Hsieh Yan-Yan Ng Wei-Sung Li Chung-Yuh Tzeng Tsai-Yi Hsu Wan-Hsiang Huang Jen-Chieh Tsai Ameliorative Effect of Imperatorin on <i>Dermatophagoides pteronyssinus</i>-Induced Allergic Asthma by Suppressing the Th2 Response in Mice Molecules imperatorin asthma <i>Dermatophagoides pteronyssinus</i> Th2 response |
| title | Ameliorative Effect of Imperatorin on <i>Dermatophagoides pteronyssinus</i>-Induced Allergic Asthma by Suppressing the Th2 Response in Mice |
| title_full | Ameliorative Effect of Imperatorin on <i>Dermatophagoides pteronyssinus</i>-Induced Allergic Asthma by Suppressing the Th2 Response in Mice |
| title_fullStr | Ameliorative Effect of Imperatorin on <i>Dermatophagoides pteronyssinus</i>-Induced Allergic Asthma by Suppressing the Th2 Response in Mice |
| title_full_unstemmed | Ameliorative Effect of Imperatorin on <i>Dermatophagoides pteronyssinus</i>-Induced Allergic Asthma by Suppressing the Th2 Response in Mice |
| title_short | Ameliorative Effect of Imperatorin on <i>Dermatophagoides pteronyssinus</i>-Induced Allergic Asthma by Suppressing the Th2 Response in Mice |
| title_sort | ameliorative effect of imperatorin on i dermatophagoides pteronyssinus i induced allergic asthma by suppressing the th2 response in mice |
| topic | imperatorin asthma <i>Dermatophagoides pteronyssinus</i> Th2 response |
| url | https://www.mdpi.com/1420-3049/27/20/7028 |
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