THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALES
The aim of the study was to investigate the total content of melanin in carpophorus, mycelia and culture filtrate species of Basidiomycetes. Methods. Extraction of melanin mycological material carried by its alkali hydrolysis 2 N NaOH solution in the ratio of 1:10, followed by precipitation with con...
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| Format: | Article |
| Language: | English |
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Odessa I. I. Mechnikov National University
2013-09-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
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| Online Access: | http://mbt.onu.edu.ua/article/view/48942 |
| _version_ | 1818886594164162560 |
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| author | А. К. Велигодська О. В. Федотов |
| author_facet | А. К. Велигодська О. В. Федотов |
| author_sort | А. К. Велигодська |
| collection | DOAJ |
| description | The aim of the study was to investigate the total content of melanin in carpophorus, mycelia and culture filtrate species of Basidiomycetes. Methods. Extraction of melanin mycological material carried by its alkali hydrolysis 2 N NaOH solution in the ratio of 1:10, followed by precipitation with concentrated HCl. The precipitate after the addition of hydrochloric acid dissolved in 5 ml of 2 N solution of NaOH. The total pigment content was determined by spectrophotometric method and were calculated using a calibration curve (by pyrocatechol). The melanin content in the culture filtrate was determined by the direct colorimetry CF. Results. The total melanin content in fruiting bodies of 50 species of Basidiomycetes, 27 of which are belonging to order Polyporales and 23 to order Agaricales was investigated. The comparative analysis on the basis of the obtained data showed the highest total melanin content in Ganoderma applanatum, Fistulina hepatica and Laetiporus sulfureus сarpophorus. Dynamics of growth and carotenoid synthesis in mycelium and cultural filtrate of 30 strains of 10 basidiomycetes species isolated in mycelial culture was studied on glucosepeptone medium. Conclusion. Selected strains of Fomes fomentarius and Lentinus edodes are promising for further studies to optimize the culture conditions for mycelial melanin origin. |
| first_indexed | 2024-12-19T16:23:49Z |
| format | Article |
| id | doaj.art-4cbb7c0ba8b64bfd99887afad4ef9249 |
| institution | Directory Open Access Journal |
| issn | 2076-0558 2307-4663 |
| language | English |
| last_indexed | 2024-12-19T16:23:49Z |
| publishDate | 2013-09-01 |
| publisher | Odessa I. I. Mechnikov National University |
| record_format | Article |
| series | Mìkrobìologìâ ì Bìotehnologìâ |
| spelling | doaj.art-4cbb7c0ba8b64bfd99887afad4ef92492022-12-21T20:14:25ZengOdessa I. I. Mechnikov National UniversityMìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632013-09-0103(23)728310.18524/2307-4663.2013.3(23).4894248942THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALESА. К. Велигодська0О. В. Федотов1Донецький національний університетДонецький національний університетThe aim of the study was to investigate the total content of melanin in carpophorus, mycelia and culture filtrate species of Basidiomycetes. Methods. Extraction of melanin mycological material carried by its alkali hydrolysis 2 N NaOH solution in the ratio of 1:10, followed by precipitation with concentrated HCl. The precipitate after the addition of hydrochloric acid dissolved in 5 ml of 2 N solution of NaOH. The total pigment content was determined by spectrophotometric method and were calculated using a calibration curve (by pyrocatechol). The melanin content in the culture filtrate was determined by the direct colorimetry CF. Results. The total melanin content in fruiting bodies of 50 species of Basidiomycetes, 27 of which are belonging to order Polyporales and 23 to order Agaricales was investigated. The comparative analysis on the basis of the obtained data showed the highest total melanin content in Ganoderma applanatum, Fistulina hepatica and Laetiporus sulfureus сarpophorus. Dynamics of growth and carotenoid synthesis in mycelium and cultural filtrate of 30 strains of 10 basidiomycetes species isolated in mycelial culture was studied on glucosepeptone medium. Conclusion. Selected strains of Fomes fomentarius and Lentinus edodes are promising for further studies to optimize the culture conditions for mycelial melanin origin.http://mbt.onu.edu.ua/article/view/48942базидіоміцетикарпофоримеланіниміцелійкультуральний фільтрат |
| spellingShingle | А. К. Велигодська О. В. Федотов THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALES Mìkrobìologìâ ì Bìotehnologìâ базидіоміцети карпофори меланіни міцелій культуральний фільтрат |
| title | THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALES |
| title_full | THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALES |
| title_fullStr | THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALES |
| title_full_unstemmed | THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALES |
| title_short | THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALES |
| title_sort | total melanins content of basidiomycetes orders polyporales and agaricales |
| topic | базидіоміцети карпофори меланіни міцелій культуральний фільтрат |
| url | http://mbt.onu.edu.ua/article/view/48942 |
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