Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach
The diversity of beta-lactam antibiotic resistance genes, with particular emphasis on carbapenemase genes, during the treatment process at two wastewater treatment plants (WWTPs) with different levels of hospital wastewater inflow was investigated using high-throughput sequencing. An additional aspe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Environmental Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenvs.2021.738158/full |
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| author | Jakub Hubeny Sławomir Ciesielski Monika Harnisz Ewa Korzeniewska Tomasz Dulski Łukasz Jałowiecki Grażyna Płaza |
| author_facet | Jakub Hubeny Sławomir Ciesielski Monika Harnisz Ewa Korzeniewska Tomasz Dulski Łukasz Jałowiecki Grażyna Płaza |
| author_sort | Jakub Hubeny |
| collection | DOAJ |
| description | The diversity of beta-lactam antibiotic resistance genes, with particular emphasis on carbapenemase genes, during the treatment process at two wastewater treatment plants (WWTPs) with different levels of hospital wastewater inflow was investigated using high-throughput sequencing. An additional aspect of the study was to determine the taxonomic diversity of microorganisms in the studied samples. The obtained results suggest that bacteria of the Fusobacteriaceae family, not associated to date with this phenomenon, may be involved in the spread of antibiotic resistance in the environment. In samples from both wastewater treatment plants, the dominant beta-lactamase genes included blaOXA, blaGES, blaBEL, blaCfxA, and blaTEM. It is worth noting that the blaKPC and blaNDM genes were only found in untreated municipal wastewater with a higher hospital wastewater content. Moreover, an increase in the abundance of the blaIMP gene after the biological treatment stage in the studied treatment plants was found. In wastewater characterized by a higher proportion of hospital wastewater, 94 correlations were observed, while in wastewater with its lower proportion, 41 correlations were noted. Considering the above, the current research indicates that the inflow of hospital wastewater contributes to the spread of antibiotic resistance in the aquatic environment. |
| first_indexed | 2024-12-21T05:48:34Z |
| format | Article |
| id | doaj.art-4cc1f7716c324173bc9bf7865fe694ed |
| institution | Directory Open Access Journal |
| issn | 2296-665X |
| language | English |
| last_indexed | 2024-12-21T05:48:34Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Environmental Science |
| spelling | doaj.art-4cc1f7716c324173bc9bf7865fe694ed2022-12-21T19:14:03ZengFrontiers Media S.A.Frontiers in Environmental Science2296-665X2021-10-01910.3389/fenvs.2021.738158738158Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic ApproachJakub Hubeny0Sławomir Ciesielski1Monika Harnisz2Ewa Korzeniewska3Tomasz Dulski4Łukasz Jałowiecki5Grażyna Płaza6Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Environmental Biotechnology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Environmental Biotechnology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandMicrobiology Unit, Institute for Ecology of Industrial Areas, Katowice, PolandFaculty of Organization and Management, Silesian University of Technology, Gliwice, PolandThe diversity of beta-lactam antibiotic resistance genes, with particular emphasis on carbapenemase genes, during the treatment process at two wastewater treatment plants (WWTPs) with different levels of hospital wastewater inflow was investigated using high-throughput sequencing. An additional aspect of the study was to determine the taxonomic diversity of microorganisms in the studied samples. The obtained results suggest that bacteria of the Fusobacteriaceae family, not associated to date with this phenomenon, may be involved in the spread of antibiotic resistance in the environment. In samples from both wastewater treatment plants, the dominant beta-lactamase genes included blaOXA, blaGES, blaBEL, blaCfxA, and blaTEM. It is worth noting that the blaKPC and blaNDM genes were only found in untreated municipal wastewater with a higher hospital wastewater content. Moreover, an increase in the abundance of the blaIMP gene after the biological treatment stage in the studied treatment plants was found. In wastewater characterized by a higher proportion of hospital wastewater, 94 correlations were observed, while in wastewater with its lower proportion, 41 correlations were noted. Considering the above, the current research indicates that the inflow of hospital wastewater contributes to the spread of antibiotic resistance in the aquatic environment.https://www.frontiersin.org/articles/10.3389/fenvs.2021.738158/fullwastewaterhospital wastewaterantibiotic resistance genesenvironmental resistomebeta-lactamasescarbapenemases |
| spellingShingle | Jakub Hubeny Sławomir Ciesielski Monika Harnisz Ewa Korzeniewska Tomasz Dulski Łukasz Jałowiecki Grażyna Płaza Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach Frontiers in Environmental Science wastewater hospital wastewater antibiotic resistance genes environmental resistome beta-lactamases carbapenemases |
| title | Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach |
| title_full | Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach |
| title_fullStr | Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach |
| title_full_unstemmed | Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach |
| title_short | Impact of Hospital Wastewater on the Occurrence and Diversity of Beta-Lactamase Genes During Wastewater Treatment with an Emphasis on Carbapenemase Genes: A Metagenomic Approach |
| title_sort | impact of hospital wastewater on the occurrence and diversity of beta lactamase genes during wastewater treatment with an emphasis on carbapenemase genes a metagenomic approach |
| topic | wastewater hospital wastewater antibiotic resistance genes environmental resistome beta-lactamases carbapenemases |
| url | https://www.frontiersin.org/articles/10.3389/fenvs.2021.738158/full |
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