Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation
Abstract Anaerobic fungi reside in the gut of herbivore and synergize with associated methanogenic archaea to decompose ingested plant biomass. Despite their potential for use in bioconversion industry, only a few natural fungus–methanogen co-cultures have been isolated and characterized. In this st...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-08-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0459-1 |
| _version_ | 1818693291534712832 |
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| author | Ya-Qin Wei Hong-Jian Yang Rui-Jun Long Zhi-Ye Wang Bin-Bin Cao Qin-Chang Ren Tian-Tian Wu |
| author_facet | Ya-Qin Wei Hong-Jian Yang Rui-Jun Long Zhi-Ye Wang Bin-Bin Cao Qin-Chang Ren Tian-Tian Wu |
| author_sort | Ya-Qin Wei |
| collection | DOAJ |
| description | Abstract Anaerobic fungi reside in the gut of herbivore and synergize with associated methanogenic archaea to decompose ingested plant biomass. Despite their potential for use in bioconversion industry, only a few natural fungus–methanogen co-cultures have been isolated and characterized. In this study we identified three co-cultures of Piromyces with Methanobrevibacter ruminantium from the rumen of yaks grazing on the Qinghai Tibetan Plateau. The representative co-culture, namely (Piromyces + M. ruminantium) Yak-G18, showed remarkable polysaccharide hydrolase production, especially xylanase. Consequently, it was able to degrade various lignocellulose substrates with a biodegrading capability superior to most previously identified fungus or fungus–methanogen co-culture isolates. End-product profiling analysis validated the beneficial metabolic impact of associated methanogen on fungus as revealed by high-yield production of methane and acetate and sustained growth on lignocellulose. Together, our data demonstrated a great potential of (Piromyces + M. ruminantium) Yak-G18 co-culture for use in industrial bioconversion of lignocellulosic biomass. |
| first_indexed | 2024-12-17T13:11:21Z |
| format | Article |
| id | doaj.art-4cc8be8105124010b7103d4378f4f118 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-17T13:11:21Z |
| publishDate | 2017-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-4cc8be8105124010b7103d4378f4f1182022-12-21T21:47:06ZengSpringerOpenAMB Express2191-08552017-08-017111210.1186/s13568-017-0459-1Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradationYa-Qin Wei0Hong-Jian Yang1Rui-Jun Long2Zhi-Ye Wang3Bin-Bin Cao4Qin-Chang Ren5Tian-Tian Wu6Key Laboratory of Microbial Resources Exploitation and Application, Gansu Province, Institute of Biological Research, Gansu Academy of SciencesState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversitySchool of Life Sciences, Lanzhou UniversityKey Laboratory of Microbial Resources Exploitation and Application, Gansu Province, Institute of Biological Research, Gansu Academy of SciencesState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityAbstract Anaerobic fungi reside in the gut of herbivore and synergize with associated methanogenic archaea to decompose ingested plant biomass. Despite their potential for use in bioconversion industry, only a few natural fungus–methanogen co-cultures have been isolated and characterized. In this study we identified three co-cultures of Piromyces with Methanobrevibacter ruminantium from the rumen of yaks grazing on the Qinghai Tibetan Plateau. The representative co-culture, namely (Piromyces + M. ruminantium) Yak-G18, showed remarkable polysaccharide hydrolase production, especially xylanase. Consequently, it was able to degrade various lignocellulose substrates with a biodegrading capability superior to most previously identified fungus or fungus–methanogen co-culture isolates. End-product profiling analysis validated the beneficial metabolic impact of associated methanogen on fungus as revealed by high-yield production of methane and acetate and sustained growth on lignocellulose. Together, our data demonstrated a great potential of (Piromyces + M. ruminantium) Yak-G18 co-culture for use in industrial bioconversion of lignocellulosic biomass.http://link.springer.com/article/10.1186/s13568-017-0459-1YakAnaerobic fungusMethanogenLignocelluloseBioconversion |
| spellingShingle | Ya-Qin Wei Hong-Jian Yang Rui-Jun Long Zhi-Ye Wang Bin-Bin Cao Qin-Chang Ren Tian-Tian Wu Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation AMB Express Yak Anaerobic fungus Methanogen Lignocellulose Bioconversion |
| title | Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation |
| title_full | Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation |
| title_fullStr | Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation |
| title_full_unstemmed | Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation |
| title_short | Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation |
| title_sort | characterization of natural co cultures of piromyces with methanobrevibacter ruminantium from yaks grazing on the qinghai tibetan plateau a microbial consortium with high potential in plant biomass degradation |
| topic | Yak Anaerobic fungus Methanogen Lignocellulose Bioconversion |
| url | http://link.springer.com/article/10.1186/s13568-017-0459-1 |
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