FoxO and rotund form a binding complex governing wing polyphenism in planthoppers
Summary: Wing polyphenism is found in a variety of insects and offers an attractive model system for studying the evolutionary significance of dispersal. The Forkhead box O (FoxO) transcription factor (TF) acts as a wing-morph switch that directs wing buds developing into long-winged (LW) or short-w...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223012592 |
| _version_ | 1797774542664368128 |
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| author | Sun-Jie Chen Jin-Li Zhang Wen-Jing Ma Hui-Jie Wu Yang Li Xing-Xing Shen Hai-Jun Xu |
| author_facet | Sun-Jie Chen Jin-Li Zhang Wen-Jing Ma Hui-Jie Wu Yang Li Xing-Xing Shen Hai-Jun Xu |
| author_sort | Sun-Jie Chen |
| collection | DOAJ |
| description | Summary: Wing polyphenism is found in a variety of insects and offers an attractive model system for studying the evolutionary significance of dispersal. The Forkhead box O (FoxO) transcription factor (TF) acts as a wing-morph switch that directs wing buds developing into long-winged (LW) or short-winged morphs in wing-dimorphic planthoppers, yet the regulatory mechanism of the FoxO module remains elusive. Here, we identified the zinc finger TF rotund as a potential wing-morph regulator via transcriptomic analysis and phenotypic screening in the brown plathopper, Nilaparvata lugens. RNA interference-mediated knockdown of rotund antagonized the LW development derived from in the context of FoxO depletion or the activation of the insulin/insulin-like growth factor signaling cascade, reversing long wings into intermediate wings. In vitro binding assays indicated that rotund physically binds to FoxO to form the FoxO combinatorial code. These findings broaden our understanding of the complexity of transcriptional regulation governing wing polyphenism in insects. |
| first_indexed | 2024-03-12T22:21:39Z |
| format | Article |
| id | doaj.art-4ccec6d6283b4c79a6144ec898761ab7 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:21:39Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-4ccec6d6283b4c79a6144ec898761ab72023-07-23T04:55:51ZengElsevieriScience2589-00422023-07-01267107182FoxO and rotund form a binding complex governing wing polyphenism in planthoppersSun-Jie Chen0Jin-Li Zhang1Wen-Jing Ma2Hui-Jie Wu3Yang Li4Xing-Xing Shen5Hai-Jun Xu6State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University; 866 Yu-Hang-Tang Avenue, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University; 866 Yu-Hang-Tang Avenue, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University; 866 Yu-Hang-Tang Avenue, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University; 866 Yu-Hang-Tang Avenue, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University; 866 Yu-Hang-Tang Avenue, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University; 866 Yu-Hang-Tang Avenue, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University; 866 Yu-Hang-Tang Avenue, Hangzhou 310058, China; Corresponding authorSummary: Wing polyphenism is found in a variety of insects and offers an attractive model system for studying the evolutionary significance of dispersal. The Forkhead box O (FoxO) transcription factor (TF) acts as a wing-morph switch that directs wing buds developing into long-winged (LW) or short-winged morphs in wing-dimorphic planthoppers, yet the regulatory mechanism of the FoxO module remains elusive. Here, we identified the zinc finger TF rotund as a potential wing-morph regulator via transcriptomic analysis and phenotypic screening in the brown plathopper, Nilaparvata lugens. RNA interference-mediated knockdown of rotund antagonized the LW development derived from in the context of FoxO depletion or the activation of the insulin/insulin-like growth factor signaling cascade, reversing long wings into intermediate wings. In vitro binding assays indicated that rotund physically binds to FoxO to form the FoxO combinatorial code. These findings broaden our understanding of the complexity of transcriptional regulation governing wing polyphenism in insects.http://www.sciencedirect.com/science/article/pii/S2589004223012592EntomologyDevelopmental geneticsPhenotypingMolecular interactionTranscriptomics |
| spellingShingle | Sun-Jie Chen Jin-Li Zhang Wen-Jing Ma Hui-Jie Wu Yang Li Xing-Xing Shen Hai-Jun Xu FoxO and rotund form a binding complex governing wing polyphenism in planthoppers iScience Entomology Developmental genetics Phenotyping Molecular interaction Transcriptomics |
| title | FoxO and rotund form a binding complex governing wing polyphenism in planthoppers |
| title_full | FoxO and rotund form a binding complex governing wing polyphenism in planthoppers |
| title_fullStr | FoxO and rotund form a binding complex governing wing polyphenism in planthoppers |
| title_full_unstemmed | FoxO and rotund form a binding complex governing wing polyphenism in planthoppers |
| title_short | FoxO and rotund form a binding complex governing wing polyphenism in planthoppers |
| title_sort | foxo and rotund form a binding complex governing wing polyphenism in planthoppers |
| topic | Entomology Developmental genetics Phenotyping Molecular interaction Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223012592 |
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