Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen
The purpose of this study was to determine the effects of apigenin and astragalus polysaccharides on the cryopreservation of bovine semen. Apigenin, astragalus polysaccharides, or their combination were added to a frozen diluent of bovine semen. Afterwards, Computer Assisted Semen Analysis <b>...
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MDPI AG
2021-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1506 |
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| author | Hongtao Wang Ping Lu Chongshan Yuan Jing Zhao Hongyu Liu Wenfa Lu Jun Wang |
| author_facet | Hongtao Wang Ping Lu Chongshan Yuan Jing Zhao Hongyu Liu Wenfa Lu Jun Wang |
| author_sort | Hongtao Wang |
| collection | DOAJ |
| description | The purpose of this study was to determine the effects of apigenin and astragalus polysaccharides on the cryopreservation of bovine semen. Apigenin, astragalus polysaccharides, or their combination were added to a frozen diluent of bovine semen. Afterwards, Computer Assisted Semen Analysis <b>(</b>CASA), membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-Px, MDA, and ROS detection were conducted. The results showed that adding 0.2 mmol/L AP or 0.5 mg/mL APS could improve the quality of frozen sperm. Compared to 0.2 mmol/L AP alone, the combination of 0.2 mmol/L AP and 0.3 mg/mL APS significantly increased the total motility (TM), average path distance (DAP), straight line distance (DSL), average path velocity (VAP), curvilinear velocity (VCL), wobble (WOB), and sperm CAT and SOD levels (<i>p</i> < 0.05), while reducing the ROS and MDA levels (<i>p</i> < 0.05). These results indicated that the addition of 0.2 mmol/L AP or 0.5 mg/mL APS alone has a protective effect on the freezing of bovine semen. Compared to the addition of 0.2 mmol/L AP, a combination of 0.2 mmol/L AP and 0.3 mg/mL APS could further improve the quality of frozen semen. |
| first_indexed | 2024-03-10T11:09:12Z |
| format | Article |
| id | doaj.art-4cd363322e864a5c97394c9618297cbf |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T11:09:12Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4cd363322e864a5c97394c9618297cbf2023-11-21T20:55:59ZengMDPI AGAnimals2076-26152021-05-01116150610.3390/ani11061506Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull SemenHongtao Wang0Ping Lu1Chongshan Yuan2Jing Zhao3Hongyu Liu4Wenfa Lu5Jun Wang6Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, ChinaThe purpose of this study was to determine the effects of apigenin and astragalus polysaccharides on the cryopreservation of bovine semen. Apigenin, astragalus polysaccharides, or their combination were added to a frozen diluent of bovine semen. Afterwards, Computer Assisted Semen Analysis <b>(</b>CASA), membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-Px, MDA, and ROS detection were conducted. The results showed that adding 0.2 mmol/L AP or 0.5 mg/mL APS could improve the quality of frozen sperm. Compared to 0.2 mmol/L AP alone, the combination of 0.2 mmol/L AP and 0.3 mg/mL APS significantly increased the total motility (TM), average path distance (DAP), straight line distance (DSL), average path velocity (VAP), curvilinear velocity (VCL), wobble (WOB), and sperm CAT and SOD levels (<i>p</i> < 0.05), while reducing the ROS and MDA levels (<i>p</i> < 0.05). These results indicated that the addition of 0.2 mmol/L AP or 0.5 mg/mL APS alone has a protective effect on the freezing of bovine semen. Compared to the addition of 0.2 mmol/L AP, a combination of 0.2 mmol/L AP and 0.3 mg/mL APS could further improve the quality of frozen semen.https://www.mdpi.com/2076-2615/11/6/1506apigeninastragalus polysaccharidebull semencryopreservation |
| spellingShingle | Hongtao Wang Ping Lu Chongshan Yuan Jing Zhao Hongyu Liu Wenfa Lu Jun Wang Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen Animals apigenin astragalus polysaccharide bull semen cryopreservation |
| title | Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen |
| title_full | Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen |
| title_fullStr | Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen |
| title_full_unstemmed | Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen |
| title_short | Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen |
| title_sort | effects of apigenin and astragalus polysaccharide on the cryopreservation of bull semen |
| topic | apigenin astragalus polysaccharide bull semen cryopreservation |
| url | https://www.mdpi.com/2076-2615/11/6/1506 |
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