Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore
Hydrogen, electric energy production, and metal toxic bioremediation are some of the biotechnological applications of sulfate-reducing organisms, which potentially depend on the sulfide produced. In this study, offshore of Yucatan, the capacity to produce hydrogen sulfide using microbial consortia f...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/10/3/436 |
| _version_ | 1797470292099989504 |
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| author | Roberto Briones-Gallardo Muriel González-Muñoz Itza García-Bautista David Valdés-Lozano Tanit Toledano-Thompson Erik Polanco-Lugo Renata Rivera-Madrid Ruby Valdez-Ojeda |
| author_facet | Roberto Briones-Gallardo Muriel González-Muñoz Itza García-Bautista David Valdés-Lozano Tanit Toledano-Thompson Erik Polanco-Lugo Renata Rivera-Madrid Ruby Valdez-Ojeda |
| author_sort | Roberto Briones-Gallardo |
| collection | DOAJ |
| description | Hydrogen, electric energy production, and metal toxic bioremediation are some of the biotechnological applications of sulfate-reducing organisms, which potentially depend on the sulfide produced. In this study, offshore of Yucatan, the capacity to produce hydrogen sulfide using microbial consortia from marine sediment (SC469, PD102, SD636) in batch reactors was evaluated. Kinetic tests were characterized by lactate oxidation to acetate, propionate, CO<sub>2</sub> and methane. The inoculum SC469, located in open-ocean, differed strongly in microbial diversity and showed better performance in substrate utilization with the highest hydrogen sulfide production (246 mmolg<sup>−1</sup> VSS) at a specific hydrogen sulfide rate of 113 mmol g<sup>−1</sup> VSS d<sup>−1</sup> with a 0.79 molar ratio of sulfate/lactate. Sulfate-reducing microbial consortia enriched in the laboratory from marine sediments collected offshore in Yucatan and with a moderate eutrophication index, differed strongly in microbial diversity with loss of microorganisms with greater capacity for degradation of organic macromolecules. The sulfate-reducing microorganisms were characterized using Illumina MiSeq technology and were mainly Desulfomicrobium, Clostridium and Desulfobacter. |
| first_indexed | 2024-03-09T19:34:31Z |
| format | Article |
| id | doaj.art-4cd7dd2566dd4c0599272a5096599946 |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-09T19:34:31Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-4cd7dd2566dd4c0599272a50965999462023-11-24T01:58:47ZengMDPI AGJournal of Marine Science and Engineering2077-13122022-03-0110343610.3390/jmse10030436Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments OffshoreRoberto Briones-Gallardo0Muriel González-Muñoz1Itza García-Bautista2David Valdés-Lozano3Tanit Toledano-Thompson4Erik Polanco-Lugo5Renata Rivera-Madrid6Ruby Valdez-Ojeda7Instituto de Metalurgia-Facultad de Ingeniería, Universidad Autónoma de San Luis Potosí, Sierra Leona 550, San Luis Potosí C.P. 78280, San Luis Potosí, MexicoUnidad Energía Renovable, Centro de Investigación Científica de Yucatán, Carretera Sierra Papacal-Chuburná Puerto, Sierra Papacal C.P. 97302, Yucatán, MexicoUnidad Energía Renovable, Centro de Investigación Científica de Yucatán, Carretera Sierra Papacal-Chuburná Puerto, Sierra Papacal C.P. 97302, Yucatán, MexicoUnidad Mérida, Centro de Investigación y de Estudios Avanzados, Mérida C.P. 97205, Yucatán, MexicoUnidad Energía Renovable, Centro de Investigación Científica de Yucatán, Carretera Sierra Papacal-Chuburná Puerto, Sierra Papacal C.P. 97302, Yucatán, MexicoFacultad de Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil, Mérida C.P. 97100, Yucatán, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida C.P. 97205, Yucatán, MexicoUnidad Energía Renovable, Centro de Investigación Científica de Yucatán, Carretera Sierra Papacal-Chuburná Puerto, Sierra Papacal C.P. 97302, Yucatán, MexicoHydrogen, electric energy production, and metal toxic bioremediation are some of the biotechnological applications of sulfate-reducing organisms, which potentially depend on the sulfide produced. In this study, offshore of Yucatan, the capacity to produce hydrogen sulfide using microbial consortia from marine sediment (SC469, PD102, SD636) in batch reactors was evaluated. Kinetic tests were characterized by lactate oxidation to acetate, propionate, CO<sub>2</sub> and methane. The inoculum SC469, located in open-ocean, differed strongly in microbial diversity and showed better performance in substrate utilization with the highest hydrogen sulfide production (246 mmolg<sup>−1</sup> VSS) at a specific hydrogen sulfide rate of 113 mmol g<sup>−1</sup> VSS d<sup>−1</sup> with a 0.79 molar ratio of sulfate/lactate. Sulfate-reducing microbial consortia enriched in the laboratory from marine sediments collected offshore in Yucatan and with a moderate eutrophication index, differed strongly in microbial diversity with loss of microorganisms with greater capacity for degradation of organic macromolecules. The sulfate-reducing microorganisms were characterized using Illumina MiSeq technology and were mainly Desulfomicrobium, Clostridium and Desulfobacter.https://www.mdpi.com/2077-1312/10/3/436marine sedimentshydrogen sulfide productionsulfate-reducing bacteria |
| spellingShingle | Roberto Briones-Gallardo Muriel González-Muñoz Itza García-Bautista David Valdés-Lozano Tanit Toledano-Thompson Erik Polanco-Lugo Renata Rivera-Madrid Ruby Valdez-Ojeda Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore Journal of Marine Science and Engineering marine sediments hydrogen sulfide production sulfate-reducing bacteria |
| title | Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore |
| title_full | Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore |
| title_fullStr | Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore |
| title_full_unstemmed | Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore |
| title_short | Hydrogen Sulfide Production with a Microbial Consortium Isolated from Marine Sediments Offshore |
| title_sort | hydrogen sulfide production with a microbial consortium isolated from marine sediments offshore |
| topic | marine sediments hydrogen sulfide production sulfate-reducing bacteria |
| url | https://www.mdpi.com/2077-1312/10/3/436 |
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