Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii)
Objective: This study analyzes the mycobiome in wild and captive Sumatran orangutans. Materials and Methods: Nine orangutan feces samples from the wild and nine from captivity were divided into three repeats from 11- to 15-year-olds in good health. The Illumina platform for analysis of ITS bioinfor...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2023-06-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | https://bdvets.org/JAVAR/?mno=128878 |
| _version_ | 1797774737820090368 |
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| author | Safika Safika Agustin Indrawati Usamah Afif Rahmat Hidayat Titiek Sunartatie |
| author_facet | Safika Safika Agustin Indrawati Usamah Afif Rahmat Hidayat Titiek Sunartatie |
| author_sort | Safika Safika |
| collection | DOAJ |
| description | Objective: This study analyzes the mycobiome in wild and captive Sumatran orangutans.
Materials and Methods: Nine orangutan feces samples from the wild and nine from captivity were divided into three repeats from 11- to 15-year-olds in good health. The Illumina platform for analysis of ITS bioinformatics was used according to the Qiime2 and CCMetagen approaches.
Results: Wild Sumatran orangutans include 53% Ascomycota, 38% uncultured fungi, and 4% Basidiomycota. Orangutans in captivity are 57% Ascomycota, 26% uncultured fungi, and 2% Basidiomycota. Based on genus level, uncultured Neurospora (31%), Penicillium (10%), Aspergillus (3%), Fusarium (3%), Candida (2%), Cutaneotrichosporon (2%), and Limonomyces (2%) are found in wild orangutans. The most prevalent genus among captivity orangutans is Aspergillus (32%), followed by fungal sp. (11%), Lasiodiplodia (18%), Devriesia (2%), and Sordariomycetes (2%). According to the Chao1 diversity index and Shannon and Simpson, there was no significant difference between wild and captive Sumatran orangutans.
Conclusion: Neurospora is unique to wild Sumatran orangutans, although Aspergillus predom¬inates in captive orangutans. We hypothesize that the gut mycobiome of wild orangutans will resemble that of orangutans in captivity. The excellent range of food sources in the forest does not result in the prevalence of fungi in the typical gut microbiome. [J Adv Vet Anim Res 2023; 10(2.000): 228-236] |
| first_indexed | 2024-03-12T22:25:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-03-12T22:25:34Z |
| publishDate | 2023-06-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-4cd915eee0b54fbea494cda5ca2343222023-07-22T06:03:10ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102023-06-0110222823610.5455/javar.2023.j673128878Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii)Safika Safika0Agustin Indrawati1Usamah Afif2Rahmat Hidayat3Titiek Sunartatie4Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia.Objective: This study analyzes the mycobiome in wild and captive Sumatran orangutans. Materials and Methods: Nine orangutan feces samples from the wild and nine from captivity were divided into three repeats from 11- to 15-year-olds in good health. The Illumina platform for analysis of ITS bioinformatics was used according to the Qiime2 and CCMetagen approaches. Results: Wild Sumatran orangutans include 53% Ascomycota, 38% uncultured fungi, and 4% Basidiomycota. Orangutans in captivity are 57% Ascomycota, 26% uncultured fungi, and 2% Basidiomycota. Based on genus level, uncultured Neurospora (31%), Penicillium (10%), Aspergillus (3%), Fusarium (3%), Candida (2%), Cutaneotrichosporon (2%), and Limonomyces (2%) are found in wild orangutans. The most prevalent genus among captivity orangutans is Aspergillus (32%), followed by fungal sp. (11%), Lasiodiplodia (18%), Devriesia (2%), and Sordariomycetes (2%). According to the Chao1 diversity index and Shannon and Simpson, there was no significant difference between wild and captive Sumatran orangutans. Conclusion: Neurospora is unique to wild Sumatran orangutans, although Aspergillus predom¬inates in captive orangutans. We hypothesize that the gut mycobiome of wild orangutans will resemble that of orangutans in captivity. The excellent range of food sources in the forest does not result in the prevalence of fungi in the typical gut microbiome. [J Adv Vet Anim Res 2023; 10(2.000): 228-236]https://bdvets.org/JAVAR/?mno=128878captivity sumatran orangutans; gut microbiota; mycobiome; pongo abelii; wild sumatran orangutans |
| spellingShingle | Safika Safika Agustin Indrawati Usamah Afif Rahmat Hidayat Titiek Sunartatie Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii) Journal of Advanced Veterinary and Animal Research captivity sumatran orangutans; gut microbiota; mycobiome; pongo abelii; wild sumatran orangutans |
| title | Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii) |
| title_full | Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii) |
| title_fullStr | Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii) |
| title_full_unstemmed | Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii) |
| title_short | Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii) |
| title_sort | metagenomic analysis of mycobiome in wild and captivity sumatran orangutans pongo abelii |
| topic | captivity sumatran orangutans; gut microbiota; mycobiome; pongo abelii; wild sumatran orangutans |
| url | https://bdvets.org/JAVAR/?mno=128878 |
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