Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile <i>Acidithiobacillus ferrooxidans</i> Strain DC
The family of Nramp (natural resistance-associated macrophage protein) metal ion transporter functions in diverse organisms from bacteria to humans. <i>Acidithiobacillus ferrooxidans</i> (<i>At. ferrooxidans</i>) is a Gram-negative bacterium that lives at pH 2 in high concent...
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MDPI AG
2020-06-01
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| Series: | Minerals |
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| Online Access: | https://www.mdpi.com/2075-163X/10/6/544 |
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| author | Bo Miao Li Shen Xueduan Liu Weimin Zeng Xueling Wu |
| author_facet | Bo Miao Li Shen Xueduan Liu Weimin Zeng Xueling Wu |
| author_sort | Bo Miao |
| collection | DOAJ |
| description | The family of Nramp (natural resistance-associated macrophage protein) metal ion transporter functions in diverse organisms from bacteria to humans. <i>Acidithiobacillus ferrooxidans</i> (<i>At. ferrooxidans</i>) is a Gram-negative bacterium that lives at pH 2 in high concentrations of soluble ferrous ion (600 mM). The AFE_2126 protein of <i>At. ferrooxidans</i> of the Dachang Copper Mine (DC) was analyzed by bioinformatics software or online tools, showing that it was highly homologous to the Nramp family, and its subcellular localization was predicted to locate in the cytoplasmic membrane. Transcriptional study revealed that AFE_2126 was expressed by Fe<sup>2+</sup>-limiting conditions in <i>At. ferrooxidans</i> DC. It can be concluded that the AFE_2126 protein may function in ferrous ion transport into the cells. Based on the <i>Δ</i>pH of the cytoplasmic membrane between the periplasm (pH 3.5) and the cytoplasm (pH 6.5), it can be concluded that Fe<sup>2+</sup> is transported in the direction identical to that of the H<sup>+</sup> gradient. This study indirectly confirmed that the function of Nramp in <i>At. ferrooxidans</i> DC can transport divalent iron ions. |
| first_indexed | 2024-03-10T19:06:58Z |
| format | Article |
| id | doaj.art-4cdb5d25539e4ee0a689c1720a471b73 |
| institution | Directory Open Access Journal |
| issn | 2075-163X |
| language | English |
| last_indexed | 2024-03-10T19:06:58Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Minerals |
| spelling | doaj.art-4cdb5d25539e4ee0a689c1720a471b732023-11-20T04:02:43ZengMDPI AGMinerals2075-163X2020-06-0110654410.3390/min10060544Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile <i>Acidithiobacillus ferrooxidans</i> Strain DCBo Miao0Li Shen1Xueduan Liu2Weimin Zeng3Xueling Wu4School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, ChinaSchool of Minerals Processing and Bioengineering, Central South University, Changsha 410083, ChinaSchool of Minerals Processing and Bioengineering, Central South University, Changsha 410083, ChinaSchool of Minerals Processing and Bioengineering, Central South University, Changsha 410083, ChinaSchool of Minerals Processing and Bioengineering, Central South University, Changsha 410083, ChinaThe family of Nramp (natural resistance-associated macrophage protein) metal ion transporter functions in diverse organisms from bacteria to humans. <i>Acidithiobacillus ferrooxidans</i> (<i>At. ferrooxidans</i>) is a Gram-negative bacterium that lives at pH 2 in high concentrations of soluble ferrous ion (600 mM). The AFE_2126 protein of <i>At. ferrooxidans</i> of the Dachang Copper Mine (DC) was analyzed by bioinformatics software or online tools, showing that it was highly homologous to the Nramp family, and its subcellular localization was predicted to locate in the cytoplasmic membrane. Transcriptional study revealed that AFE_2126 was expressed by Fe<sup>2+</sup>-limiting conditions in <i>At. ferrooxidans</i> DC. It can be concluded that the AFE_2126 protein may function in ferrous ion transport into the cells. Based on the <i>Δ</i>pH of the cytoplasmic membrane between the periplasm (pH 3.5) and the cytoplasm (pH 6.5), it can be concluded that Fe<sup>2+</sup> is transported in the direction identical to that of the H<sup>+</sup> gradient. This study indirectly confirmed that the function of Nramp in <i>At. ferrooxidans</i> DC can transport divalent iron ions.https://www.mdpi.com/2075-163X/10/6/544acidophilic bacteriumAFE_2126Nramp familymetal ion transportersmagnetic nanoparticlesmultireference genes |
| spellingShingle | Bo Miao Li Shen Xueduan Liu Weimin Zeng Xueling Wu Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile <i>Acidithiobacillus ferrooxidans</i> Strain DC Minerals acidophilic bacterium AFE_2126 Nramp family metal ion transporters magnetic nanoparticles multireference genes |
| title | Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile <i>Acidithiobacillus ferrooxidans</i> Strain DC |
| title_full | Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile <i>Acidithiobacillus ferrooxidans</i> Strain DC |
| title_fullStr | Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile <i>Acidithiobacillus ferrooxidans</i> Strain DC |
| title_full_unstemmed | Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile <i>Acidithiobacillus ferrooxidans</i> Strain DC |
| title_short | Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile <i>Acidithiobacillus ferrooxidans</i> Strain DC |
| title_sort | bioinformatics and transcriptional study of the nramp gene in the extreme acidophile i acidithiobacillus ferrooxidans i strain dc |
| topic | acidophilic bacterium AFE_2126 Nramp family metal ion transporters magnetic nanoparticles multireference genes |
| url | https://www.mdpi.com/2075-163X/10/6/544 |
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