Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus.
Motilin and ghrelin constitute a peptide family, and these hormones are important for the regulation of gastrointestinal motility. In this study, we examined the effect of motilin and ghrelin on gastric acid secretion in anesthetized suncus (house musk shrew, Suncus murinus), a ghrelin- and motilin-...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4482737?pdf=render |
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| author | Chayon Goswami Yoshiaki Shimada Makoto Yoshimura Anupom Mondal Sen-ichi Oda Toru Tanaka Takafumi Sakai Ichiro Sakata |
| author_facet | Chayon Goswami Yoshiaki Shimada Makoto Yoshimura Anupom Mondal Sen-ichi Oda Toru Tanaka Takafumi Sakai Ichiro Sakata |
| author_sort | Chayon Goswami |
| collection | DOAJ |
| description | Motilin and ghrelin constitute a peptide family, and these hormones are important for the regulation of gastrointestinal motility. In this study, we examined the effect of motilin and ghrelin on gastric acid secretion in anesthetized suncus (house musk shrew, Suncus murinus), a ghrelin- and motilin-producing mammal. We first established a gastric lumen-perfusion system in the suncus and confirmed that intravenous (i.v.) administration of histamine (1 mg/kg body weight) stimulated acid secretion. Motilin (0.1, 1.0, and 10 μg/kg BW) stimulated the acid output in a dose-dependent manner in suncus, whereas ghrelin (0.1, 1.0, and 10 μg/kg BW) alone did not induce acid output. Furthermore, in comparison with the vehicle administration, the co-administration of low-dose (1 μg/kg BW) motilin and ghrelin significantly stimulated gastric acid secretion, whereas either motilin (1 μg/kg BW) or ghrelin (1 μg/kg BW) alone did not significantly induce gastric acid secretion. This indicates an additive role of ghrelin in motilin-induced gastric acid secretion. We then investigated the pathways of motilin/motilin and ghrelin-stimulated acid secretion using receptor antagonists. Treatment with YM 022 (a CCK-B receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist) had no effect on motilin or motilin-ghrelin co-administration-induced acid output. In contrast, famotidine (a histamine H2 receptor antagonist) completely inhibited motilin-stimulated acid secretion and co-administration of motilin and ghrelin induced gastric acid output. This is the first report demonstrating that motilin stimulates gastric secretion in mammals. Our results also suggest that motilin and co-administration of motilin and ghrelin stimulate gastric acid secretion via the histamine-mediated pathway in suncus. |
| first_indexed | 2024-12-10T11:27:39Z |
| format | Article |
| id | doaj.art-4cdd19e4940646e3b2d2293aab369a37 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T11:27:39Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4cdd19e4940646e3b2d2293aab369a372022-12-22T01:50:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e013155410.1371/journal.pone.0131554Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus.Chayon GoswamiYoshiaki ShimadaMakoto YoshimuraAnupom MondalSen-ichi OdaToru TanakaTakafumi SakaiIchiro SakataMotilin and ghrelin constitute a peptide family, and these hormones are important for the regulation of gastrointestinal motility. In this study, we examined the effect of motilin and ghrelin on gastric acid secretion in anesthetized suncus (house musk shrew, Suncus murinus), a ghrelin- and motilin-producing mammal. We first established a gastric lumen-perfusion system in the suncus and confirmed that intravenous (i.v.) administration of histamine (1 mg/kg body weight) stimulated acid secretion. Motilin (0.1, 1.0, and 10 μg/kg BW) stimulated the acid output in a dose-dependent manner in suncus, whereas ghrelin (0.1, 1.0, and 10 μg/kg BW) alone did not induce acid output. Furthermore, in comparison with the vehicle administration, the co-administration of low-dose (1 μg/kg BW) motilin and ghrelin significantly stimulated gastric acid secretion, whereas either motilin (1 μg/kg BW) or ghrelin (1 μg/kg BW) alone did not significantly induce gastric acid secretion. This indicates an additive role of ghrelin in motilin-induced gastric acid secretion. We then investigated the pathways of motilin/motilin and ghrelin-stimulated acid secretion using receptor antagonists. Treatment with YM 022 (a CCK-B receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist) had no effect on motilin or motilin-ghrelin co-administration-induced acid output. In contrast, famotidine (a histamine H2 receptor antagonist) completely inhibited motilin-stimulated acid secretion and co-administration of motilin and ghrelin induced gastric acid output. This is the first report demonstrating that motilin stimulates gastric secretion in mammals. Our results also suggest that motilin and co-administration of motilin and ghrelin stimulate gastric acid secretion via the histamine-mediated pathway in suncus.http://europepmc.org/articles/PMC4482737?pdf=render |
| spellingShingle | Chayon Goswami Yoshiaki Shimada Makoto Yoshimura Anupom Mondal Sen-ichi Oda Toru Tanaka Takafumi Sakai Ichiro Sakata Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus. PLoS ONE |
| title | Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus. |
| title_full | Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus. |
| title_fullStr | Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus. |
| title_full_unstemmed | Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus. |
| title_short | Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus. |
| title_sort | motilin stimulates gastric acid secretion in coordination with ghrelin in suncus murinus |
| url | http://europepmc.org/articles/PMC4482737?pdf=render |
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