Characterization of Gut Microbiome in the Mud Snail <i>Cipangopaludina cathayensis</i> in Response to High-Temperature Stress
The mud snail <i>Cipangopaludina cathayensis</i> is a widely distributed species in China. Particularly in Guangxi province, mud snail farming contributes significantly to the economic development. However, global warming in recent decades poses a serious threat to global aquaculture pro...
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MDPI AG
2022-09-01
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| Series: | Animals |
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| author | Yang-Yang Wu Chun-Xing Cheng Liu Yang Quan-Qing Ye Wen-Hong Li Jiao-Yun Jiang |
| author_facet | Yang-Yang Wu Chun-Xing Cheng Liu Yang Quan-Qing Ye Wen-Hong Li Jiao-Yun Jiang |
| author_sort | Yang-Yang Wu |
| collection | DOAJ |
| description | The mud snail <i>Cipangopaludina cathayensis</i> is a widely distributed species in China. Particularly in Guangxi province, mud snail farming contributes significantly to the economic development. However, global warming in recent decades poses a serious threat to global aquaculture production. The rising water temperature is harmful to aquatic animals. The present study explored the effects of high temperature on the intestinal microbiota of <i>C. cathayensis</i>. Snail intestinal samples were collected from the control and high-temperature groups on days 3 and 7 to determine the gut microbiota composition and diversity. Gut bacterial community composition was investigated using high-throughput sequencing of the V3–V4 region of bacterial 16S rRNA genes. Our results suggested that thermal stress altered the gut microbiome structure of <i>C. cathayensis</i>. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were dominant in <i>C. cathayensis</i> gut microbiota. The T2 treatment (32 ± 1 °C, day 7) significantly decreased the relative abundance of Firmicutes, Actinobacteria, and Deinococcus-Thermus. In T2, the abundance of several genera of putatively beneficial bacteria (<i>Pseudomonas</i>, <i>Aeromonas</i>, <i>Rhodobacter</i>, and <i>Bacteroides</i>) decreased, whereas the abundance of <i>Halomonas</i>—a pathogenic bacterial genus—increased. The functional prediction results indicated that T2 treatment inhibited some carbohydrate metabolism pathways and induced certain disease-related pathways (e.g., those related to systemic lupus erythematosus, <i>Vibrio cholerae</i> infection, hypertrophic cardiomyopathy, and shigellosis). Thus, high temperature profoundly affected the community structure and function of <i>C. cathayensis</i> gut microbiota. The results provide insights into the mechanisms associated with response of <i>C. cathayensis</i> intestinal microbiota to global warming. |
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| spelling | doaj.art-4cddb2f17b064bb1be68c55ec3ebf1062023-11-23T14:41:38ZengMDPI AGAnimals2076-26152022-09-011218236110.3390/ani12182361Characterization of Gut Microbiome in the Mud Snail <i>Cipangopaludina cathayensis</i> in Response to High-Temperature StressYang-Yang Wu0Chun-Xing Cheng1Liu Yang2Quan-Qing Ye3Wen-Hong Li4Jiao-Yun Jiang5Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, ChinaCollege of Animal Science and Technology, Guangxi University, 100 Daxue Road, Nanning 530004, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, ChinaThe mud snail <i>Cipangopaludina cathayensis</i> is a widely distributed species in China. Particularly in Guangxi province, mud snail farming contributes significantly to the economic development. However, global warming in recent decades poses a serious threat to global aquaculture production. The rising water temperature is harmful to aquatic animals. The present study explored the effects of high temperature on the intestinal microbiota of <i>C. cathayensis</i>. Snail intestinal samples were collected from the control and high-temperature groups on days 3 and 7 to determine the gut microbiota composition and diversity. Gut bacterial community composition was investigated using high-throughput sequencing of the V3–V4 region of bacterial 16S rRNA genes. Our results suggested that thermal stress altered the gut microbiome structure of <i>C. cathayensis</i>. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were dominant in <i>C. cathayensis</i> gut microbiota. The T2 treatment (32 ± 1 °C, day 7) significantly decreased the relative abundance of Firmicutes, Actinobacteria, and Deinococcus-Thermus. In T2, the abundance of several genera of putatively beneficial bacteria (<i>Pseudomonas</i>, <i>Aeromonas</i>, <i>Rhodobacter</i>, and <i>Bacteroides</i>) decreased, whereas the abundance of <i>Halomonas</i>—a pathogenic bacterial genus—increased. The functional prediction results indicated that T2 treatment inhibited some carbohydrate metabolism pathways and induced certain disease-related pathways (e.g., those related to systemic lupus erythematosus, <i>Vibrio cholerae</i> infection, hypertrophic cardiomyopathy, and shigellosis). Thus, high temperature profoundly affected the community structure and function of <i>C. cathayensis</i> gut microbiota. The results provide insights into the mechanisms associated with response of <i>C. cathayensis</i> intestinal microbiota to global warming.https://www.mdpi.com/2076-2615/12/18/2361thermal stress<i>Cipangopaludina Cathayensis</i>gut microbiotahigh-throughput sequencing |
| spellingShingle | Yang-Yang Wu Chun-Xing Cheng Liu Yang Quan-Qing Ye Wen-Hong Li Jiao-Yun Jiang Characterization of Gut Microbiome in the Mud Snail <i>Cipangopaludina cathayensis</i> in Response to High-Temperature Stress Animals thermal stress <i>Cipangopaludina Cathayensis</i> gut microbiota high-throughput sequencing |
| title | Characterization of Gut Microbiome in the Mud Snail <i>Cipangopaludina cathayensis</i> in Response to High-Temperature Stress |
| title_full | Characterization of Gut Microbiome in the Mud Snail <i>Cipangopaludina cathayensis</i> in Response to High-Temperature Stress |
| title_fullStr | Characterization of Gut Microbiome in the Mud Snail <i>Cipangopaludina cathayensis</i> in Response to High-Temperature Stress |
| title_full_unstemmed | Characterization of Gut Microbiome in the Mud Snail <i>Cipangopaludina cathayensis</i> in Response to High-Temperature Stress |
| title_short | Characterization of Gut Microbiome in the Mud Snail <i>Cipangopaludina cathayensis</i> in Response to High-Temperature Stress |
| title_sort | characterization of gut microbiome in the mud snail i cipangopaludina cathayensis i in response to high temperature stress |
| topic | thermal stress <i>Cipangopaludina Cathayensis</i> gut microbiota high-throughput sequencing |
| url | https://www.mdpi.com/2076-2615/12/18/2361 |
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