Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells
<p>Abstract</p> <p>Background</p> <p>The digallic acid (DGA) purified from <it>Pistacia lentiscus</it>. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.</p> <p>Methods</p> <p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-06-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://www.cancerci.com/content/12/1/26 |
| _version_ | 1818136029561880576 |
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| author | Bhouri Wissem Boubaker Jihed Skandrani Ines Ghedira Kamel Chekir Ghedira Leila |
| author_facet | Bhouri Wissem Boubaker Jihed Skandrani Ines Ghedira Kamel Chekir Ghedira Leila |
| author_sort | Bhouri Wissem |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The digallic acid (DGA) purified from <it>Pistacia lentiscus</it>. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.</p> <p>Methods</p> <p>We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities.</p> <p>Results</p> <p>The inhibition of lymphoblastoid cell proliferation was noted from 8.5 μg/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner.</p> <p>Conclusion</p> <p>In summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent.</p> |
| first_indexed | 2024-12-11T09:33:55Z |
| format | Article |
| id | doaj.art-4cded45e029b44c38744571f0783cd70 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-11T09:33:55Z |
| publishDate | 2012-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-4cded45e029b44c38744571f0783cd702022-12-22T01:12:56ZengBMCCancer Cell International1475-28672012-06-011212610.1186/1475-2867-12-26Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cellsBhouri WissemBoubaker JihedSkandrani InesGhedira KamelChekir Ghedira Leila<p>Abstract</p> <p>Background</p> <p>The digallic acid (DGA) purified from <it>Pistacia lentiscus</it>. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.</p> <p>Methods</p> <p>We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities.</p> <p>Results</p> <p>The inhibition of lymphoblastoid cell proliferation was noted from 8.5 μg/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner.</p> <p>Conclusion</p> <p>In summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent.</p>http://www.cancerci.com/content/12/1/26Caspase activityDNA fragmentationDigallic acidPARP |
| spellingShingle | Bhouri Wissem Boubaker Jihed Skandrani Ines Ghedira Kamel Chekir Ghedira Leila Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells Cancer Cell International Caspase activity DNA fragmentation Digallic acid PARP |
| title | Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells |
| title_full | Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells |
| title_fullStr | Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells |
| title_full_unstemmed | Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells |
| title_short | Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells |
| title_sort | investigation of the apoptotic way induced by digallic acid in human lymphoblastoid tk6 cells |
| topic | Caspase activity DNA fragmentation Digallic acid PARP |
| url | http://www.cancerci.com/content/12/1/26 |
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