Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls
Tithonia diversifolia is a medicinal plant from the Municipality of Suchiapa, Chiapas, Mexico, that according to local folk medicine is considered useful in the treatment of gastric ulcers. The aim of the present study was to investigate the gastroprotective activity of T. diversifolia by using an e...
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MDPI AG
2011-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/16/1/665/ |
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| author | Jesús Arrieta Juan Rodríguez-Silverio María Elena Sánchez-Mendoza Leticia Cruz Antonio Adelfo Reyes-Ramírez Luis Martínez Jiménez |
| author_facet | Jesús Arrieta Juan Rodríguez-Silverio María Elena Sánchez-Mendoza Leticia Cruz Antonio Adelfo Reyes-Ramírez Luis Martínez Jiménez |
| author_sort | Jesús Arrieta |
| collection | DOAJ |
| description | Tithonia diversifolia is a medicinal plant from the Municipality of Suchiapa, Chiapas, Mexico, that according to local folk medicine is considered useful in the treatment of gastric ulcers. The aim of the present study was to investigate the gastroprotective activity of T. diversifolia by using an ethanol-induced gastric ulcer experimental model in male Wistar rats. The results showed that T. diversifolia had gastroprotective activity, and that the dichloromethane extract had the highest protective activity (close to 90% when using doses between 10 to 100 mg/kg), and that further the compound tagitinin C isolated from this extract was the main active gastroprotective agent. Rats treated with tagitinin C suspended in Tween 80 at 1, 3, 10 and 30 mg/kg showed 37.7, 70.1, 100, and 100% gastroprotection, respectively. The effect elicited by tagitinin C (30 mg/kg) was not attenuated by pretreatment with either NG-nitro-L-arginine methyl ester (70 mg/kg, i.p.), a nitric oxide (NO) synthase inhibitor, N-ethylmaleimide (10 mg/kg, s.c.), a blocker of sulfhydryl groups, or indomethacin (10 mg/kg, s.c.), a blocker of prostaglandin synthesis, which suggests that the gastroprotective mechanism of action of this sesquiterpene lactone does not involve NO, sulfhydryl groups or prostaglandins. |
| first_indexed | 2024-04-14T04:03:34Z |
| format | Article |
| id | doaj.art-4ce19f3213464298937391d99e0076f1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-14T04:03:34Z |
| publishDate | 2011-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4ce19f3213464298937391d99e0076f12022-12-22T02:13:28ZengMDPI AGMolecules1420-30492011-01-0116166567410.3390/molecules16010665Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and SulfhydrylsJesús ArrietaJuan Rodríguez-SilverioMaría Elena Sánchez-MendozaLeticia Cruz AntonioAdelfo Reyes-RamírezLuis Martínez JiménezTithonia diversifolia is a medicinal plant from the Municipality of Suchiapa, Chiapas, Mexico, that according to local folk medicine is considered useful in the treatment of gastric ulcers. The aim of the present study was to investigate the gastroprotective activity of T. diversifolia by using an ethanol-induced gastric ulcer experimental model in male Wistar rats. The results showed that T. diversifolia had gastroprotective activity, and that the dichloromethane extract had the highest protective activity (close to 90% when using doses between 10 to 100 mg/kg), and that further the compound tagitinin C isolated from this extract was the main active gastroprotective agent. Rats treated with tagitinin C suspended in Tween 80 at 1, 3, 10 and 30 mg/kg showed 37.7, 70.1, 100, and 100% gastroprotection, respectively. The effect elicited by tagitinin C (30 mg/kg) was not attenuated by pretreatment with either NG-nitro-L-arginine methyl ester (70 mg/kg, i.p.), a nitric oxide (NO) synthase inhibitor, N-ethylmaleimide (10 mg/kg, s.c.), a blocker of sulfhydryl groups, or indomethacin (10 mg/kg, s.c.), a blocker of prostaglandin synthesis, which suggests that the gastroprotective mechanism of action of this sesquiterpene lactone does not involve NO, sulfhydryl groups or prostaglandins.http://www.mdpi.com/1420-3049/16/1/665/Tithonia diversifoliaAsteraceaetagitinin Cgastroprotectionmedicinal plants |
| spellingShingle | Jesús Arrieta Juan Rodríguez-Silverio María Elena Sánchez-Mendoza Leticia Cruz Antonio Adelfo Reyes-Ramírez Luis Martínez Jiménez Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls Molecules Tithonia diversifolia Asteraceae tagitinin C gastroprotection medicinal plants |
| title | Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls |
| title_full | Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls |
| title_fullStr | Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls |
| title_full_unstemmed | Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls |
| title_short | Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls |
| title_sort | bioassay guided isolation of an anti ulcer compound tagitinin c from tithonia diversifolia role of nitric oxide prostaglandins and sulfhydryls |
| topic | Tithonia diversifolia Asteraceae tagitinin C gastroprotection medicinal plants |
| url | http://www.mdpi.com/1420-3049/16/1/665/ |
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