Methicillin Resistance, Vancomycin Intermediate and Vancomycin Resistance Staphylococcus aureus Prevalence in a Tertiary Care Hospital of Punjab, India
Introduction: Multidrug resistance Staphylococcus aureus (S.aureus) is a very notorious pathogen to develop drug resistance. After methicillin, resistance to vancomycin and linezolid were being reported from various areas of the world including India which is a serious issue. Aim: To determine pr...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Pvt. Ltd.
2019-07-01
|
| Series: | National Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | http://njlm.net/article_fulltext.asp?issn=0973-709x&year=2019&month=July&volume=8&issue=3&page=MO01-MO03&id=2352 |
| _version_ | 1818487969505345536 |
|---|---|
| author | Kiranjeet Kaur Amarjit Kaur Gill Mandeep Kaur |
| author_facet | Kiranjeet Kaur Amarjit Kaur Gill Mandeep Kaur |
| author_sort | Kiranjeet Kaur |
| collection | DOAJ |
| description | Introduction: Multidrug resistance Staphylococcus aureus (S.aureus) is a very notorious pathogen to develop drug resistance. After methicillin, resistance to vancomycin and linezolid were being reported from various areas of the world including India which is a serious issue.
Aim: To determine prevalence of Methicillin Resistant S. aureus (MRSA), Vancomycin Intermediate S. aureus (VISA) and Vancomycin Resistant S. aureus (VRSA) in a tertiary care hospital of Punjab, India.
Materials and Methods: The study was conducted in a tertiary care hospital from June 2016 to May 2017. S. aureus isolated from various clinical samples were identified as per standard protocol. Antimicrobial susceptibility was done by Kirby bauer disc diffusion method. MRSA detection was done using 30 μg cefoxitin disc on Mueller Hinton Agar (MHA). Vancomycin resistance was determined by agar dilution method. Vancomycin MIC <2 μg/mL reported as sensitive, MIC 4-8 μg/mL reported as VISA and MIC >16 μg/mL reported as VRSA.
Results: Out of 162 S. aureus isolates, 85 (52.4%) were female and 77 (47.5%) were male patients. Maximum 96 (59.2%) S. aureus were isolated from pus and pus swab. An 83 (51.2%) were MRSA, 19 (11.7%) were VISA and 4 (2.46%) were VRSA. Ampicillin 130 (80.0%), ciprofloxacin 102 (62.9%), co-trimoxazole 100 (61.7%) and erythromycim 96 (59%) were most resistant whereas tigecycline 162 (100%) was most sensitive followed by linezolid 154 (95%) and tecoplanin 153 (94%).
Conclusion: There is high prevalence of MRSA and an increasing MIC of vancomycin is reported in S. aureus which is big challenge for antimicrobial therapy. Performing culture and sensitivity in infectious cases in the hospital will help in reserving these drugs for those cases where all other drugs are resistant. |
| first_indexed | 2024-12-10T16:45:06Z |
| format | Article |
| id | doaj.art-4ce21000871e4ff0bc7d72439261a643 |
| institution | Directory Open Access Journal |
| issn | 2277-8551 2455-6882 |
| language | English |
| last_indexed | 2024-12-10T16:45:06Z |
| publishDate | 2019-07-01 |
| publisher | JCDR Research and Publications Pvt. Ltd. |
| record_format | Article |
| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-4ce21000871e4ff0bc7d72439261a6432022-12-22T01:41:04ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822019-07-01083MO01MO0310.7860/NJLM/2019/41137:2352Methicillin Resistance, Vancomycin Intermediate and Vancomycin Resistance Staphylococcus aureus Prevalence in a Tertiary Care Hospital of Punjab, IndiaKiranjeet Kaur0Amarjit Kaur Gill1Mandeep Kaur2Assistant Professor, Department of Microbiology, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India.Professor and Head, Department of Microbiology, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India.Assistant Professor, Department of Microbiology, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India.Introduction: Multidrug resistance Staphylococcus aureus (S.aureus) is a very notorious pathogen to develop drug resistance. After methicillin, resistance to vancomycin and linezolid were being reported from various areas of the world including India which is a serious issue. Aim: To determine prevalence of Methicillin Resistant S. aureus (MRSA), Vancomycin Intermediate S. aureus (VISA) and Vancomycin Resistant S. aureus (VRSA) in a tertiary care hospital of Punjab, India. Materials and Methods: The study was conducted in a tertiary care hospital from June 2016 to May 2017. S. aureus isolated from various clinical samples were identified as per standard protocol. Antimicrobial susceptibility was done by Kirby bauer disc diffusion method. MRSA detection was done using 30 μg cefoxitin disc on Mueller Hinton Agar (MHA). Vancomycin resistance was determined by agar dilution method. Vancomycin MIC <2 μg/mL reported as sensitive, MIC 4-8 μg/mL reported as VISA and MIC >16 μg/mL reported as VRSA. Results: Out of 162 S. aureus isolates, 85 (52.4%) were female and 77 (47.5%) were male patients. Maximum 96 (59.2%) S. aureus were isolated from pus and pus swab. An 83 (51.2%) were MRSA, 19 (11.7%) were VISA and 4 (2.46%) were VRSA. Ampicillin 130 (80.0%), ciprofloxacin 102 (62.9%), co-trimoxazole 100 (61.7%) and erythromycim 96 (59%) were most resistant whereas tigecycline 162 (100%) was most sensitive followed by linezolid 154 (95%) and tecoplanin 153 (94%). Conclusion: There is high prevalence of MRSA and an increasing MIC of vancomycin is reported in S. aureus which is big challenge for antimicrobial therapy. Performing culture and sensitivity in infectious cases in the hospital will help in reserving these drugs for those cases where all other drugs are resistant.http://njlm.net/article_fulltext.asp?issn=0973-709x&year=2019&month=July&volume=8&issue=3&page=MO01-MO03&id=2352antimicrobial susceptibilityinfectionminimum inhibitory concentrationmultidrug resistance |
| spellingShingle | Kiranjeet Kaur Amarjit Kaur Gill Mandeep Kaur Methicillin Resistance, Vancomycin Intermediate and Vancomycin Resistance Staphylococcus aureus Prevalence in a Tertiary Care Hospital of Punjab, India National Journal of Laboratory Medicine antimicrobial susceptibility infection minimum inhibitory concentration multidrug resistance |
| title | Methicillin Resistance, Vancomycin Intermediate and Vancomycin Resistance Staphylococcus aureus Prevalence in a Tertiary Care Hospital of Punjab, India |
| title_full | Methicillin Resistance, Vancomycin Intermediate and Vancomycin Resistance Staphylococcus aureus Prevalence in a Tertiary Care Hospital of Punjab, India |
| title_fullStr | Methicillin Resistance, Vancomycin Intermediate and Vancomycin Resistance Staphylococcus aureus Prevalence in a Tertiary Care Hospital of Punjab, India |
| title_full_unstemmed | Methicillin Resistance, Vancomycin Intermediate and Vancomycin Resistance Staphylococcus aureus Prevalence in a Tertiary Care Hospital of Punjab, India |
| title_short | Methicillin Resistance, Vancomycin Intermediate and Vancomycin Resistance Staphylococcus aureus Prevalence in a Tertiary Care Hospital of Punjab, India |
| title_sort | methicillin resistance vancomycin intermediate and vancomycin resistance staphylococcus aureus prevalence in a tertiary care hospital of punjab india |
| topic | antimicrobial susceptibility infection minimum inhibitory concentration multidrug resistance |
| url | http://njlm.net/article_fulltext.asp?issn=0973-709x&year=2019&month=July&volume=8&issue=3&page=MO01-MO03&id=2352 |
| work_keys_str_mv | AT kiranjeetkaur methicillinresistancevancomycinintermediateandvancomycinresistancestaphylococcusaureusprevalenceinatertiarycarehospitalofpunjabindia AT amarjitkaurgill methicillinresistancevancomycinintermediateandvancomycinresistancestaphylococcusaureusprevalenceinatertiarycarehospitalofpunjabindia AT mandeepkaur methicillinresistancevancomycinintermediateandvancomycinresistancestaphylococcusaureusprevalenceinatertiarycarehospitalofpunjabindia |