Innovative Biotechnology for Generation of Cardiac Tissue
Heart transplantation remains the only curative treatment for end-stage heart failure. This life-saving option continues to be limited by the low number of organ donors, graft rejection and adverse effects of immunosuppressants. Engineering bioartificial hearts from acellular native-derived scaffold...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/11/12/5603 |
| _version_ | 1797529829273239552 |
|---|---|
| author | Greta Ionela Barbulescu Florina Maria Bojin Valentin Laurentiu Ordodi Iacob Daniel Goje Taddeus Paul Buica Oana Isabella Gavriliuc Flavia Baderca Teodora Hoinoiu Virgil Paunescu |
| author_facet | Greta Ionela Barbulescu Florina Maria Bojin Valentin Laurentiu Ordodi Iacob Daniel Goje Taddeus Paul Buica Oana Isabella Gavriliuc Flavia Baderca Teodora Hoinoiu Virgil Paunescu |
| author_sort | Greta Ionela Barbulescu |
| collection | DOAJ |
| description | Heart transplantation remains the only curative treatment for end-stage heart failure. This life-saving option continues to be limited by the low number of organ donors, graft rejection and adverse effects of immunosuppressants. Engineering bioartificial hearts from acellular native-derived scaffolds and stem cells has gained attention because of its potential to overcome these limitations. In this study, rat hearts (<i>n</i> = 20) were decellularized by means of coronary perfusion with 1% sodium dodecyl sulfate (SDS) in a modified Langendorff device. The electrical field behavior of the SDS molecule was studied and it was assumed that when applying an alternating current, the exposure time of the tissue to the detergent might decrease. To repopulate the decellularized extracellular matrix (ECM), human mesenchymal stem cells (hMSCs) were used, induced to differentiate into cardiomyocytes (CMs) with 5-azacytidine (5-aza). The results showed no cellular debris and an intact ECM following decellularization. Decellularization in the presence of an electric field proved to be faster, decreasing the potential risk of ECM damage due to the detergent. After cell seeding and culturing of eight scaffolds with hMSCs, the recellularization process was analyzed using optic microscopy (OM), which showed cells suggestive for CMs. This study presents a novel and efficient decellularization protocol using an electric field and suggests that hMSCs can be useful in the generation of a bioartificial heart. |
| first_indexed | 2024-03-10T10:19:25Z |
| format | Article |
| id | doaj.art-4ce3a9a39b904c8597ef179bc2f938f7 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T10:19:25Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4ce3a9a39b904c8597ef179bc2f938f72023-11-22T00:31:52ZengMDPI AGApplied Sciences2076-34172021-06-011112560310.3390/app11125603Innovative Biotechnology for Generation of Cardiac TissueGreta Ionela Barbulescu0Florina Maria Bojin1Valentin Laurentiu Ordodi2Iacob Daniel Goje3Taddeus Paul Buica4Oana Isabella Gavriliuc5Flavia Baderca6Teodora Hoinoiu7Virgil Paunescu8Immuno-Physiology and Biotechnologies Center, Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy, No. 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaImmuno-Physiology and Biotechnologies Center, Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy, No. 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaClinical Emergency County Hospital “Pius Brinzeu” Timisoara, Center for Gene and Cellular Therapies in the Treatment of Cancer Timisoara-OncoGen, No. 156 Liviu Rebreanu, 300723 Timisoara, RomaniaDepartment of Internal Medicine I, Medical Semiology I, “Victor Babes” University of Medicine and Pharmacy, No. 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaClinical Emergency County Hospital “Pius Brinzeu” Timisoara, Center for Gene and Cellular Therapies in the Treatment of Cancer Timisoara-OncoGen, No. 156 Liviu Rebreanu, 300723 Timisoara, RomaniaImmuno-Physiology and Biotechnologies Center, Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy, No. 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaDepartment of Microscopic Morphology, Histology, “Victor Babes” University of Medicine and Pharmacy, No. 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaDepartment of Clinical Practical Skills, “Victor Babes” University of Medicine and Pharmacy, No. 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaImmuno-Physiology and Biotechnologies Center, Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy, No. 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaHeart transplantation remains the only curative treatment for end-stage heart failure. This life-saving option continues to be limited by the low number of organ donors, graft rejection and adverse effects of immunosuppressants. Engineering bioartificial hearts from acellular native-derived scaffolds and stem cells has gained attention because of its potential to overcome these limitations. In this study, rat hearts (<i>n</i> = 20) were decellularized by means of coronary perfusion with 1% sodium dodecyl sulfate (SDS) in a modified Langendorff device. The electrical field behavior of the SDS molecule was studied and it was assumed that when applying an alternating current, the exposure time of the tissue to the detergent might decrease. To repopulate the decellularized extracellular matrix (ECM), human mesenchymal stem cells (hMSCs) were used, induced to differentiate into cardiomyocytes (CMs) with 5-azacytidine (5-aza). The results showed no cellular debris and an intact ECM following decellularization. Decellularization in the presence of an electric field proved to be faster, decreasing the potential risk of ECM damage due to the detergent. After cell seeding and culturing of eight scaffolds with hMSCs, the recellularization process was analyzed using optic microscopy (OM), which showed cells suggestive for CMs. This study presents a novel and efficient decellularization protocol using an electric field and suggests that hMSCs can be useful in the generation of a bioartificial heart.https://www.mdpi.com/2076-3417/11/12/5603cardiac decellularizationdecellularized extracellular matrixmesenchymal stem cellsregenerative medicinerecellularizationengineering bioartificial heart |
| spellingShingle | Greta Ionela Barbulescu Florina Maria Bojin Valentin Laurentiu Ordodi Iacob Daniel Goje Taddeus Paul Buica Oana Isabella Gavriliuc Flavia Baderca Teodora Hoinoiu Virgil Paunescu Innovative Biotechnology for Generation of Cardiac Tissue Applied Sciences cardiac decellularization decellularized extracellular matrix mesenchymal stem cells regenerative medicine recellularization engineering bioartificial heart |
| title | Innovative Biotechnology for Generation of Cardiac Tissue |
| title_full | Innovative Biotechnology for Generation of Cardiac Tissue |
| title_fullStr | Innovative Biotechnology for Generation of Cardiac Tissue |
| title_full_unstemmed | Innovative Biotechnology for Generation of Cardiac Tissue |
| title_short | Innovative Biotechnology for Generation of Cardiac Tissue |
| title_sort | innovative biotechnology for generation of cardiac tissue |
| topic | cardiac decellularization decellularized extracellular matrix mesenchymal stem cells regenerative medicine recellularization engineering bioartificial heart |
| url | https://www.mdpi.com/2076-3417/11/12/5603 |
| work_keys_str_mv | AT gretaionelabarbulescu innovativebiotechnologyforgenerationofcardiactissue AT florinamariabojin innovativebiotechnologyforgenerationofcardiactissue AT valentinlaurentiuordodi innovativebiotechnologyforgenerationofcardiactissue AT iacobdanielgoje innovativebiotechnologyforgenerationofcardiactissue AT taddeuspaulbuica innovativebiotechnologyforgenerationofcardiactissue AT oanaisabellagavriliuc innovativebiotechnologyforgenerationofcardiactissue AT flaviabaderca innovativebiotechnologyforgenerationofcardiactissue AT teodorahoinoiu innovativebiotechnologyforgenerationofcardiactissue AT virgilpaunescu innovativebiotechnologyforgenerationofcardiactissue |