Cold Exposure After Exercise Impedes the Neuroprotective Effects of Exercise on Thermoregulation and UCP4 Expression in an MPTP-Induced Parkinsonian Mouse Model
Moderate exercise and mild hypothermia have protective effects against brain injury and neurodegeneration. Running in a cold environment alters exercise-induced hyperthermia and outcomes; however, evaluations of post-exercise cold exposure related to exercise benefits for the brain are relatively ra...
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnins.2020.573509/full |
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| author | Yi-Ju Tsai Yue-Cih Jhong Shih-Hong Ching Yu-Ching Liao Cheng-Hsin Ching Jih-Ing Chuang Jih-Ing Chuang |
| author_facet | Yi-Ju Tsai Yue-Cih Jhong Shih-Hong Ching Yu-Ching Liao Cheng-Hsin Ching Jih-Ing Chuang Jih-Ing Chuang |
| author_sort | Yi-Ju Tsai |
| collection | DOAJ |
| description | Moderate exercise and mild hypothermia have protective effects against brain injury and neurodegeneration. Running in a cold environment alters exercise-induced hyperthermia and outcomes; however, evaluations of post-exercise cold exposure related to exercise benefits for the brain are relatively rare. We investigated the effects of 4°C cold exposure after exercise on exercise-induced thermal responses and neuroprotection in an MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced Parkinsonian mouse model. Male C57BL/6J mice were pretreated with MPTP for five consecutive days and follow-up treadmill exercise for 4 weeks. After 1-h running at a 22°C temperature, the mice were exposed to a 4°C environment for 2 h. An MPTP injection induced a transient drop in body and brain temperatures, while mild brain hypothermia was found to last for 4 weeks after MPTP treatment. Preventing brain hypothermia by exercise or 4°C exposure was associated with an improvement in MPTP-induced striatal uncoupling protein 4 (UCP4) downregulation and nigrostriatal dopaminergic neurodegeneration. However, 4°C exposure after exercise abrogated the exercise-induced beneficial effects and thermal responses in MPTP-treated mice, including a low amplitude of exercise-induced brain hyperthermia and body temperature while at rest after exercise. Our findings elucidate that post-exercise thermoregulation and UCP4 expression are important in the neuroprotective effects of exercise against MPTP toxicity. |
| first_indexed | 2024-12-21T13:41:13Z |
| format | Article |
| id | doaj.art-4ce4dc74e38143dc8a7a6ca4e8827fdb |
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| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-21T13:41:13Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-4ce4dc74e38143dc8a7a6ca4e8827fdb2022-12-21T19:02:01ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2020-09-011410.3389/fnins.2020.573509573509Cold Exposure After Exercise Impedes the Neuroprotective Effects of Exercise on Thermoregulation and UCP4 Expression in an MPTP-Induced Parkinsonian Mouse ModelYi-Ju Tsai0Yue-Cih Jhong1Shih-Hong Ching2Yu-Ching Liao3Cheng-Hsin Ching4Jih-Ing Chuang5Jih-Ing Chuang6Department of Physical Therapy, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Physiology, College of Medicine, National Cheng Kung University, Tainan, TaiwanThe Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Physiology, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Physiology, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Physiology, College of Medicine, National Cheng Kung University, Tainan, TaiwanThe Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan, TaiwanModerate exercise and mild hypothermia have protective effects against brain injury and neurodegeneration. Running in a cold environment alters exercise-induced hyperthermia and outcomes; however, evaluations of post-exercise cold exposure related to exercise benefits for the brain are relatively rare. We investigated the effects of 4°C cold exposure after exercise on exercise-induced thermal responses and neuroprotection in an MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced Parkinsonian mouse model. Male C57BL/6J mice were pretreated with MPTP for five consecutive days and follow-up treadmill exercise for 4 weeks. After 1-h running at a 22°C temperature, the mice were exposed to a 4°C environment for 2 h. An MPTP injection induced a transient drop in body and brain temperatures, while mild brain hypothermia was found to last for 4 weeks after MPTP treatment. Preventing brain hypothermia by exercise or 4°C exposure was associated with an improvement in MPTP-induced striatal uncoupling protein 4 (UCP4) downregulation and nigrostriatal dopaminergic neurodegeneration. However, 4°C exposure after exercise abrogated the exercise-induced beneficial effects and thermal responses in MPTP-treated mice, including a low amplitude of exercise-induced brain hyperthermia and body temperature while at rest after exercise. Our findings elucidate that post-exercise thermoregulation and UCP4 expression are important in the neuroprotective effects of exercise against MPTP toxicity.https://www.frontiersin.org/article/10.3389/fnins.2020.573509/fulltreadmill exercisecold exposurebrain temperatureuncoupling protein 4Parkinson’s diseaseMPTP |
| spellingShingle | Yi-Ju Tsai Yue-Cih Jhong Shih-Hong Ching Yu-Ching Liao Cheng-Hsin Ching Jih-Ing Chuang Jih-Ing Chuang Cold Exposure After Exercise Impedes the Neuroprotective Effects of Exercise on Thermoregulation and UCP4 Expression in an MPTP-Induced Parkinsonian Mouse Model Frontiers in Neuroscience treadmill exercise cold exposure brain temperature uncoupling protein 4 Parkinson’s disease MPTP |
| title | Cold Exposure After Exercise Impedes the Neuroprotective Effects of Exercise on Thermoregulation and UCP4 Expression in an MPTP-Induced Parkinsonian Mouse Model |
| title_full | Cold Exposure After Exercise Impedes the Neuroprotective Effects of Exercise on Thermoregulation and UCP4 Expression in an MPTP-Induced Parkinsonian Mouse Model |
| title_fullStr | Cold Exposure After Exercise Impedes the Neuroprotective Effects of Exercise on Thermoregulation and UCP4 Expression in an MPTP-Induced Parkinsonian Mouse Model |
| title_full_unstemmed | Cold Exposure After Exercise Impedes the Neuroprotective Effects of Exercise on Thermoregulation and UCP4 Expression in an MPTP-Induced Parkinsonian Mouse Model |
| title_short | Cold Exposure After Exercise Impedes the Neuroprotective Effects of Exercise on Thermoregulation and UCP4 Expression in an MPTP-Induced Parkinsonian Mouse Model |
| title_sort | cold exposure after exercise impedes the neuroprotective effects of exercise on thermoregulation and ucp4 expression in an mptp induced parkinsonian mouse model |
| topic | treadmill exercise cold exposure brain temperature uncoupling protein 4 Parkinson’s disease MPTP |
| url | https://www.frontiersin.org/article/10.3389/fnins.2020.573509/full |
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