Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer
Objective To investigate the effect and mechanism of tumor-associated macrophages (TAMs) on the invasion and metastasis of cervical cancer. Methods Immunohistochemical method was used to detect the infiltration of TAMs in cervical lesions. The human mononuclear THP1 cell line was induced to transfor...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Magazine House of Cancer Research on Prevention and Treatment
2019-03-01
|
| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.0769.htm |
| _version_ | 1818543819437637632 |
|---|---|
| author | CHEN Xiaojing WANG Wei ZHOU Chenfei WEI Wenfei WU Xiangguang YAN Ruiming ZHANG Yanmei LIANG Luojiao WU Sha LIANG Li ZHONG Mei YU Yanhong |
| author_facet | CHEN Xiaojing WANG Wei ZHOU Chenfei WEI Wenfei WU Xiangguang YAN Ruiming ZHANG Yanmei LIANG Luojiao WU Sha LIANG Li ZHONG Mei YU Yanhong |
| author_sort | CHEN Xiaojing |
| collection | DOAJ |
| description | Objective To investigate the effect and mechanism of tumor-associated macrophages (TAMs) on the invasion and metastasis of cervical cancer. Methods Immunohistochemical method was used to detect the infiltration of TAMs in cervical lesions. The human mononuclear THP1 cell line was induced to transform into M2 type TAMs in vitro. TAMs were used to stimulate cervical cancer cells (SiHa and C33a). Scratch and Transwell assays were used to detect the effect of TAMs on the migration and invasion of cervical cancer cell lines. Western blot was used to detect the epithelial-mesenchymal transition and the expression of MMP-9 in cervical cancer cells. Results The number of TAM infiltration was positively correlated with the progression of cervical lesions. After stimulated by TAMs-CM, SiHa and C33a cells appeared interstitial-like changes, and the migration and invasion abilities were significantly enhanced (both P < 0.5). TAMs could down-regulate the expression of E-Cadherin, and up-regulate the expression of N-Cadherin, Vimentin and MMP-9. Conclusion The invasion of TAMs is closely related to the malignant transformation and progression of cervical epithelium. TAMs may promote the invasion and metastasis of cervical cancer cells by up-regulating the expression of MMP-9. |
| first_indexed | 2024-12-11T22:40:23Z |
| format | Article |
| id | doaj.art-4ce51db375df43a3ae53dfb49efaa6d5 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-11T22:40:23Z |
| publishDate | 2019-03-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-4ce51db375df43a3ae53dfb49efaa6d52022-12-22T00:47:49ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782019-03-0146321221710.3971/j.issn.1000-8578.2019.18.07698578.2019.18.0769Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical CancerCHEN Xiaojing0WANG Wei1ZHOU Chenfei2WEI Wenfei3WU Xiangguang4YAN Ruiming5ZHANG Yanmei6LIANG Luojiao7WU Sha8LIANG Li9ZHONG Mei10YU Yanhong11Department of Obstetrics and Gynecology, Nanfang Hospital/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, ChinaDepartment of Obstetrics and Gynecology, Nanfang Hospital/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, ChinaDepartment of Obstetrics and Gynecology, Nanfang Hospital/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, ChinaDepartment of Obstetrics and Gynecology, Nanfang Hospital/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, ChinaDepartment of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, ChinaDepartment of Obstetrics and Gynecology, Nanfang Hospital/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, ChinaDepartment of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathology, Nanfang Hospital/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, ChinaDepartment of Obstetrics and Gynecology, Nanfang Hospital/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, ChinaDepartment of Obstetrics and Gynecology, Nanfang Hospital/The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, ChinaObjective To investigate the effect and mechanism of tumor-associated macrophages (TAMs) on the invasion and metastasis of cervical cancer. Methods Immunohistochemical method was used to detect the infiltration of TAMs in cervical lesions. The human mononuclear THP1 cell line was induced to transform into M2 type TAMs in vitro. TAMs were used to stimulate cervical cancer cells (SiHa and C33a). Scratch and Transwell assays were used to detect the effect of TAMs on the migration and invasion of cervical cancer cell lines. Western blot was used to detect the epithelial-mesenchymal transition and the expression of MMP-9 in cervical cancer cells. Results The number of TAM infiltration was positively correlated with the progression of cervical lesions. After stimulated by TAMs-CM, SiHa and C33a cells appeared interstitial-like changes, and the migration and invasion abilities were significantly enhanced (both P < 0.5). TAMs could down-regulate the expression of E-Cadherin, and up-regulate the expression of N-Cadherin, Vimentin and MMP-9. Conclusion The invasion of TAMs is closely related to the malignant transformation and progression of cervical epithelium. TAMs may promote the invasion and metastasis of cervical cancer cells by up-regulating the expression of MMP-9.http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.0769.htmcervical cancertumor microenvironmenttumor-associated macrophagesinvasionmetastasis |
| spellingShingle | CHEN Xiaojing WANG Wei ZHOU Chenfei WEI Wenfei WU Xiangguang YAN Ruiming ZHANG Yanmei LIANG Luojiao WU Sha LIANG Li ZHONG Mei YU Yanhong Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer Zhongliu Fangzhi Yanjiu cervical cancer tumor microenvironment tumor-associated macrophages invasion metastasis |
| title | Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer |
| title_full | Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer |
| title_fullStr | Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer |
| title_full_unstemmed | Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer |
| title_short | Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer |
| title_sort | tumor associated macrophages promote invasion and metastasis of cervical cancer |
| topic | cervical cancer tumor microenvironment tumor-associated macrophages invasion metastasis |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.0769.htm |
| work_keys_str_mv | AT chenxiaojing tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT wangwei tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT zhouchenfei tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT weiwenfei tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT wuxiangguang tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT yanruiming tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT zhangyanmei tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT liangluojiao tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT wusha tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT liangli tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT zhongmei tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer AT yuyanhong tumorassociatedmacrophagespromoteinvasionandmetastasisofcervicalcancer |